1. Antigen storage compartments in mature dendritic cells facilitate prolonged cytotoxic T lymphocyte cross-priming capacity.
- Author
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van Montfoort N, Camps MG, Khan S, Filippov DV, Weterings JJ, Griffith JM, Geuze HJ, van Hall T, Verbeek JS, Melief CJ, and Ossendorp F
- Subjects
- Animals, Antigen Presentation immunology, Cell Membrane metabolism, Dendritic Cells ultrastructure, Histocompatibility Antigens Class I immunology, Intracellular Space metabolism, Lysosomes metabolism, Lysosomes ultrastructure, Mice, Peptides immunology, Protein Stability, Receptors, Immunologic metabolism, Time Factors, Antigens immunology, Cell Compartmentation immunology, Cell Differentiation immunology, Cross-Priming immunology, Dendritic Cells cytology, Dendritic Cells immunology, T-Lymphocytes, Cytotoxic immunology
- Abstract
Dendritic cells (DCs) are crucial for priming of naive CD8(+) T lymphocytes to exogenous antigens, so-called "cross-priming." We report that exogenous protein antigen can be conserved for several days in mature DCs, coinciding with strong cytotoxic T lymphocyte cross-priming potency in vivo. After MHC class I peptide elution, protein antigen-derived peptide presentation is efficiently restored, indicating the presence of an intracellular antigen depot. We characterized this depot as a lysosome-like organelle, distinct from MHC class II compartments and recently described early endosomal compartments that allow acute antigen presentation in MHC class I. The storage compartments we report here facilitate continuous supply of MHC class I ligands. This mechanism ensures sustained cross-presentation by DCs, despite the short-lived expression of MHC class I-peptide complexes at the cell surface.
- Published
- 2009
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