1. Use of poxvirus display to select antibodies specific for complex membrane antigens.
- Author
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Smith ES, Balch LA, Scrivens M, Shi S, Wang W, Harvey CD, Cornelison AA, Gil-Moore M, Kirk RA, Mueller LL, Hall RL, Howell AP, Reilly CA, Mayer JM, Murante FG, Viggiani KA, Gersz EM, Bussler H, Keefe MR, Evans EE, Paris MJ, and Zauderer M
- Subjects
- Antibodies, Membrane Proteins, Cell Membrane, Peptide Library, Antigens
- Abstract
Antibody discovery against complex antigens is limited by the availability of a reproducible pure source of concentrated properly folded antigen. We have developed a technology to enable direct incorporation of membrane proteins such as GPCRs and into the membrane of poxvirus. The protein of interest is correctly folded and expressed in the cell-derived viral membrane and does not require any detergents or refolding before downstream use. The poxvirus is selective in which proteins are incorporated into the viral membrane, making the antigen poxvirus an antigenically cleaner target for in vitro panning. Antigen-expressing virus can be readily purified at scale and used for antibody selection using any in vitro display platform.
- Published
- 2023
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