1. High level antibody response to retrovirus-associated but not to melanocyte lineage-specific antigens in mice protected against B16 melanoma.
- Author
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Sfondrini L, Morelli D, Bodini A, Colnaghi MI, Ménard S, and Balsari A
- Subjects
- 3T3 Cells, Animals, CHO Cells, Cancer Vaccines immunology, Cell Lineage, Cloning, Molecular, Cricetinae, DNA, Complementary metabolism, Dose-Response Relationship, Drug, Female, Flow Cytometry, Fluorescent Antibody Technique, Humans, Immunoglobulin G immunology, Interferon Type I genetics, Intramolecular Oxidoreductases genetics, Leukemia Virus, Murine immunology, Mice, Mice, Inbred BALB C, Mycobacterium tuberculosis immunology, Precipitin Tests, Pregnancy Proteins genetics, Transduction, Genetic, Antibody Formation, Antigens, Viral immunology, Immunity, Melanocytes immunology, Melanoma, Experimental immunology, Melanoma, Experimental prevention & control, Retroviridae immunology
- Abstract
Mice vaccinated with Mycobacterium tuberculosis Ag38 gene-transduced B16 melanoma cells showed significant protection from intravenous challenge with parental B16 melanoma cells. No cytotoxic T-cell activity was found against melanoma cells, although the endogenous presence of the mycobacterial gene induced a preferential Th1 response. After immunization, a low serological response against melanoma cells was detected, while a high titer of antibodies directed to parental B16 cells, mainly of IgG2(a) isotype, was found in protected mice after challenge. These antibodies exhibited complement-dependent cytotoxicity against melanoma cells in vitro, while in vivo, used in passive immunization, they induced a decrease in a number of experimental B16 lung metastases. Most of the antibodies were directed against endogenous murine leukemia viruses. No reactivity against melanocyte lineage-specific antigens was observed. In particular, no reactivity was found in sera from protected mice against tyrosinase-related protein 2 (TRP-2), either stably expressed in a non-melanoma cell line or obtained by in vitro transcription-translation, or against tyrosinase, TRP-1 and gp100 antigens immunoprecipitated from B16 cells. Thus, in the B16 murine model, the presence of dominant viral antigens induces a very strong humoral response that might be protective and may inhibit or mask the presence of minor clonotypes., (Copyright 1999 Wiley-Liss, Inc.)
- Published
- 1999
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