1. Organization of the mouse GP42/Basigin gene: a member of the Ig superfamily.
- Author
-
Cheng Y, Li X, Kamholz J, and Burns FR
- Subjects
- Amino Acid Sequence, Animals, Base Sequence, Basigin, DNA, Complementary isolation & purification, Exons, Mice, Mice, Inbred BALB C, Molecular Sequence Data, Polymorphism, Genetic, Sequence Alignment, Antigens, CD, Antigens, Neoplasm, Antigens, Surface genetics, Avian Proteins, Blood Proteins, Membrane Glycoproteins genetics
- Abstract
We have mapped and sequenced the GP42/Basigin gene isolated from a Balb/C mouse genomic library. The genomic organization and upstream, putative regulatory, regions of this gene have not been previously reported. Our data show that exon 5 of the GP42/Basigin gene encodes the carboxy proximal half of the second Ig-like domain, the highly conserved transmembrane region and a portion of the cytoplasmic tail. This inclusion of Ig-like and other functional domains in a single exon is unusual. Splice junction analysis indicates that two reported alternate GP42/Basigin cDNA isoforms are likely due to cloning artifacts. In addition, we find that GP42/Basigin is polymorphic in mice. Our data also support the proposal that the transmembrane domain and portions of the cytoplasmic region of GP42/Basigin have been evolutionarily conserved.
- Published
- 1994
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