1. Entamoeba histolytica antigenic protein detected in pus aspirates from patients with amoebic liver abscess.
- Author
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Othman N, Mohamed Z, Yahya MM, Leow VM, Lim BH, and Noordin R
- Subjects
- Animals, Antibodies, Protozoan blood, Antibodies, Protozoan immunology, Blotting, Western, Cricetinae, DNA, Protozoan analysis, Electrophoresis, Polyacrylamide Gel, Entamoeba histolytica genetics, Enzyme-Linked Immunosorbent Assay, Gas Chromatography-Mass Spectrometry, Humans, Immunoglobulin G blood, Isoelectric Focusing, Liver Abscess, Amebic parasitology, Mesocricetus, Polymerase Chain Reaction, Proteomics, Protozoan Proteins genetics, Protozoan Proteins immunology, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Suppuration immunology, Suppuration parasitology, Tandem Mass Spectrometry, Antigens, Protozoan isolation & purification, Entamoeba histolytica immunology, Liver Abscess, Amebic immunology, Protozoan Proteins isolation & purification
- Abstract
Entamoeba histolytica is a causative agent of amoebic liver abscess (ALA) and is endemic in many underdeveloped countries. We investigated antigenic E. histolytica proteins in liver abscess aspirates using proteomics approach. Pus samples were first tested by real-time PCR to confirm the presence of E. histolytica DNA and the corresponding serum samples tested for E. histolytica-specific IgG by a commercial ELISA. Proteins were extracted from three and one pool(s) of pus samples from ALA and PLA (pyogenic liver abscess) patients respectively, followed by analysis using isoelectric focussing, SDS-PAGE and Western blot. Unpurified pooled serum samples from infected hamsters and pooled human amoebic-specific IgG were used as primary antibodies. The antigenic protein band was excised from the gel, digested and analysed by MALDI-TOF/TOF and LC-MS/MS. The results using both primary antibodies showed an antigenic protein band of ∼14kDa. Based on the mass spectrum analysis, putative tyrosine kinase is the most probable identification of the antigenic band., (Copyright © 2013 Elsevier Inc. All rights reserved.)
- Published
- 2013
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