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Your search keyword '"Peruccio D"' showing total 3 results
Start Over Author "Peruccio D" Topic antigens, neoplasm
3 results on '"Peruccio D"'

1. Quantitative expression of HLA class I molecules in acute non-lymphoblastic leukaemia cells.

Author

D'Alfonso S, Savoia P, Pitti G, Peruccio D, Pozzi C, Crepaldi T, Falda M, Ficara F, Resegotti L, and Momigliano Richiardi P

Subjects
Antigens, Neoplasm genetics, HLA-A Antigens genetics, HLA-B Antigens genetics, Humans, Leukemia, Myeloid, Acute genetics, Neoplasm Proteins biosynthesis, Neoplasm Proteins genetics, Neoplastic Stem Cells metabolism, RNA, Messenger genetics, RNA, Neoplasm genetics, beta 2-Microglobulin biosynthesis, beta 2-Microglobulin genetics, Antigens, Neoplasm biosynthesis, Gene Expression Regulation, Leukemic, HLA-A Antigens biosynthesis, HLA-B Antigens biosynthesis, Leukemia, Myeloid, Acute immunology, Neoplastic Stem Cells immunology
Abstract

The present study concerns a panel of 33 acute non lymphoblastic leukaemia (ANLL) patients, previously typed for HLA-A,B serological specificities and including samples with a normal HLA-A,B phenotype (3,4 detected specificities) as well as samples with missing and extra specificities. Samples were analysed at the protein and/or RNA level in order to verify whether the observed typing anomalies were due to a modified quantitative expression of class I molecules. The number of HLA-A,B assigned specificities correlated significantly with the cell surface class I expression detected by indirect immunofluorescence using the monomorphic anti-class I MoAb W6/32 (Spearman rank correlation test, P < 0.01) and with the amount of class I Heavy Chain (HC, P < 0.05) and beta-2-microglobulin (beta 2m, P < 0.05) evaluated by Western blot in whole cell extracts. The RNA analysis suggested a HC-beta 2m coordinated down regulation at the mRNA level in a patient with no assigned HLA-A,B specificities. Another patient with no detectable HLA-A,B specificities showed a low expression selectively of the beta 2m protein. The results reported here demonstrate a heterogenous quantitative HLA class I expression in ANLL blasts, analogous to results reported for solid tumours.

Published
1993
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2. Loss of surface HLA class I molecules in leukemic myeloblasts is correlated with an increased leukocyte concentration at onset.

Author

Savoia P, D'Alfonso S, Peruccio D, Isabella N, Falda M, Rasegotti L, and Richiardi P

Subjects
Adolescent, Adult, Aged, Female, Gene Expression Regulation immunology, HLA-A Antigens genetics, Humans, Leukemia, Myeloid, Acute blood, Leukocyte Count, Male, Middle Aged, Antigens, Neoplasm immunology, HLA-A Antigens analysis, Leukemia, Myeloid, Acute immunology
Abstract

Background: Reduced expression of HLA class I molecules has been demonstrated for many human neoplasias and is correlated with increased malignancy., Methods: We investigated the correlation between HLA expression on blasts and the number of peripheral blood leukocytes (WBC) at onset in 34 patients with acute non lymphoblastic (ANLL) leukemia. Leukemic blasts at onset were serologically typed for HLA-A,B specificities, and the patients were classified in three groups according to the number of detectable surface HLA antigens., Results: The amount of WBC at onset in patients lacking HLA antigens was significantly higher than in patients with no antigenic loss., Conclusions: A decrease in HLA class I molecules on the cell surface is correlated with a greater expansion of the leukemic clone.

Published
1992

3. Loss of surface HLA class I molecules in leukemic myeloblasts is correlated with an increased leukocyte concentration at onset

Author

Paola Savoia, Alfonso, Sandra D., Peruccio, D., Isabella, N., Falda, M., Rasegotti, L., and Richiardi, P.

Subjects
Adult, Male, Leukemia, Myeloid, Acute, Leukocyte Count, Adolescent, Gene Expression Regulation, HLA-A Antigens, Antigens, Neoplasm, Humans, Female, Middle Aged, Aged
Abstract

Reduced expression of HLA class I molecules has been demonstrated for many human neoplasias and is correlated with increased malignancy.We investigated the correlation between HLA expression on blasts and the number of peripheral blood leukocytes (WBC) at onset in 34 patients with acute non lymphoblastic (ANLL) leukemia. Leukemic blasts at onset were serologically typed for HLA-A,B specificities, and the patients were classified in three groups according to the number of detectable surface HLA antigens.The amount of WBC at onset in patients lacking HLA antigens was significantly higher than in patients with no antigenic loss.A decrease in HLA class I molecules on the cell surface is correlated with a greater expansion of the leukemic clone.

Published
1992

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