1. Proteomics show antigen presentation processes in human immune cells after AS03-H5N1 vaccination.
- Author
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Galassie AC, Goll JB, Samir P, Jensen TL, Hoek KL, Howard LM, Allos TM, Niu X, Gordy LE, Creech CB, Hill H, Joyce S, Edwards KM, and Link AJ
- Subjects
- Adjuvants, Immunologic, B-Lymphocytes cytology, B-Lymphocytes immunology, B-Lymphocytes metabolism, Cells, Cultured, Humans, Influenza, Human immunology, Influenza, Human prevention & control, Killer Cells, Natural cytology, Killer Cells, Natural immunology, Killer Cells, Natural metabolism, Monocytes cytology, Monocytes immunology, Monocytes metabolism, Neutrophils cytology, Neutrophils immunology, Neutrophils metabolism, Protein Interaction Maps, Proteomics, T-Lymphocytes cytology, T-Lymphocytes immunology, T-Lymphocytes metabolism, Antigen Presentation, Influenza A Virus, H5N1 Subtype immunology, Influenza Vaccines therapeutic use, Proteome metabolism
- Abstract
Adjuvants enhance immunity elicited by vaccines through mechanisms that are poorly understood. Using a systems biology approach, we investigated temporal protein expression changes in five primary human immune cell populations: neutrophils, monocytes, natural killer cells, T cells, and B cells after administration of either an Adjuvant System 03 adjuvanted or unadjuvanted split-virus H5N1 influenza vaccine. Monocytes demonstrated the strongest differential signal between vaccine groups. On day 3 post-vaccination, several antigen presentation-related pathways, including MHC class I-mediated antigen processing and presentation, were enriched in monocytes and neutrophils and expression of HLA class I proteins was increased in the Adjuvant System 03 group. We identified several protein families whose proteomic responses predicted seroprotective antibody responses (>1:40 hemagglutination inhibition titer), including inflammation and oxidative stress proteins at day 1 as well as immunoproteasome subunit (PSME1 and PSME2) and HLA class I proteins at day 3 in monocytes. While comparison between temporal proteomic and transcriptomic results showed little overlap overall, enrichment of the MHC class I antigen processing and presentation pathway in monocytes and neutrophils was confirmed by both approaches., (© 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
- Published
- 2017
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