1. Serological Characterization of Human Monocytes for HLA, B-Lymphocyte, Granulocyte, and Monocyte-Associated Antigens by Cytotoxicity Testing
- Author
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P. Zamfirescu, B. M. Carter, David S. Nathanson, Paul I. Terasaki, S. Ian Drew, Ronald Billing, and Faramarz Naiem
- Subjects
Isoantigens ,T-Lymphocytes ,Lymphocyte ,Transplantation, Heterologous ,Immunology ,Population ,Histocompatibility Testing ,Human leukocyte antigen ,Granulocyte ,Biology ,Biochemistry ,Monocytes ,Antigen ,HLA Antigens ,Genetics ,medicine ,Humans ,Transplantation, Homologous ,Immunology and Allergy ,Blood Transfusion ,education ,Pan-T antigens ,B-Lymphocytes ,education.field_of_study ,Immune Sera ,Monocyte ,Complement System Proteins ,General Medicine ,Cytotoxicity Tests, Immunologic ,medicine.anatomical_structure ,Granulocytes - Abstract
Highly enriched preparations of monocytes, B and T lymphocytes, and granulocytes from 18 normal donors were serotyped in parallel in a complement-dependent cytotoxicity assay using allogeneic and heterologous antisera defining three independent tissue antigen systems. HLA and B-lymphocyte tissue antigens were detected on human monocytes although granulocyte antigens were absent. By cytotoxicity testing the presence of Ia-like antigens on monocytes was significantly diminished compared to the autologous B-lymphocyte population and has important implications in B-lymphocyte serology. The study indentified a number of human antisera obtained from multitransfused subjects and pre- and post-transplant organ recipients that were non-HLA and appeared to define monocyte-associated antigens. The serological implications of surface antigen expression on human monocytes compared with other peripheral blood cells are discussed.
- Published
- 1978
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