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Neutral (Emmons modification) Sabouraud medium, Dermatophyte Test Medium and Littman oxgall agar, with substitutions in type of antibacterial antibiotic, were quantitatively evaluated by colony counts for ability to support growth when suspensions of trypsintreated guinea pig skin scales, infected with Trichophyton mentagrophytes, were used as inocula. Both liquid and powder form of gentamicin sulfate were used separately in place of other routinely used antibacterial antibiotics. Also evaluated were three isolation temperatures. No differences in ability to support growth were noted among media. No difference was noted between the two forms of gentamicin. No data were available on the antibacterial activities of the antibiotics because no bacterial growth was produced on any of the media. Room temperature (24 degrees-26 degrees C), 30 degrees C and 37 degrees C were found to support growth similarly as primary isolation temperatures for this fungus.