1. Fish oil administration mediates apoptosis of Walker 256 tumor cells by modulation of p53, Bcl-2, caspase-7 and caspase-3 protein expression.
- Author
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Borghetti G, Yamaguchi AA, Aikawa J, Yamazaki RK, de Brito GA, and Fernandes LC
- Subjects
- Animals, Apoptosis drug effects, Carcinoma 256, Walker genetics, Carcinoma 256, Walker metabolism, Carcinoma 256, Walker pathology, Caspase 3 genetics, Caspase 3 metabolism, Caspase 7 genetics, Caspase 7 metabolism, Coconut Oil, Injections, Subcutaneous, Male, Plant Oils pharmacology, Proto-Oncogene Proteins c-bcl-2 genetics, Proto-Oncogene Proteins c-bcl-2 metabolism, Rats, Rats, Wistar, Signal Transduction, Tumor Burden drug effects, Tumor Suppressor Protein p53 genetics, Tumor Suppressor Protein p53 metabolism, Anticarcinogenic Agents pharmacology, Carcinoma 256, Walker diet therapy, Dietary Supplements, Fish Oils pharmacology, Gene Expression Regulation, Neoplastic drug effects
- Abstract
Background: Several studies have been shown pro-apoptotic effects of fish oil (FO), rich in n-3 polyunsaturated fatty acids (n-3 PUFA) on cancer cells. Nevertheless, few in vivo experiments have provided data of its ability on apoptosis protein expression in tumor tissue. Thus, in this study we investigate the effect of FO supplementation on apoptosis protein expression in Walker 256 tumor bearing rats. Male Wistar rats were randomly assigned to three groups: fed with regular chow (W); fed regular chow supplemented with FO (WFO) or coconut fat (WCO) (1 g/kg body weight/daily). After thirty days, all animals were inoculated subcutaneously with Walker 256 tumor cells., Findings: Protein expression was done by western blotting in Walker 256 tumor tissue samples. FO decreased the Bcl-2/Bax ratio (p < 0.05) and increased the p53 (p < 0.05), cleaved caspase-7 (p < 0.05) and cleaved caspase-3 (p < 0.05) in Walker 256 tumor tissue., Conclusions: Our data suggest that the pro-apoptotic effect of FO in Walker 256 tumor is related with specifics cleaved caspases.
- Published
- 2015
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