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Echis ocellatus is one of the commonest snakes responsible for envenomation in Nigeria. Antivenom is the only effective treatment, but the country suffers from a limited supply of effective antivenom. This study therefore aimed to explore the feasibility of effective, mono-specific antibodies production through immunization in rabbits using the venom of Echis ocellatus from Nigeria. The World Health Organization guide on antivenom production was employed in the immunization and the resultant antibodies were purified using protein A agarose column chromatography. Antibody titer reached a high plateau by 2-month immunization, and SDS PAGE of the sera suggests the presence of intact immunoglobulins accompanied with the heavy (50 kDa) and light (25 kDa) chains. The venom has an intravenous LD 50 of 0.35 mg/kg in mice, and the venom lethality at a challenge dose of 2 LD 50 was effectively neutralized by the antibodies with a potency value of 0.83 mg venom per g antibodies. The antibodies also neutralized the procoagulant activity of the venom with an effective dose (ED) of 13 ± 0.66 μl, supporting its use for hemotoxic envenomation. The study establishes the feasibility of developing effective, mono-specific antibodies against the Nigerian Carpet viper. [Display omitted] • This study investigated the feasibility of producing antibodies against Echis ocellatus venom in rabbits. • The antibodies effectively neutralized the venom's lethality, with a potency value of 0.83 mg venom per gram. • The procoagulant activity was also neutralized with an effective dose (ED) of 13 ± 0.66 μL. [ABSTRACT FROM AUTHOR]