1. Assessment of the outer membrane protein profile of Vibrio parahaemolyticus using the sodium dodecyl sulfate-polyacrylamide gel electrophoresis method.
- Author
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Pattipeilohy, Mery, Amin, Moh., Lestari, Umie, and Suarsini, Endang
- Subjects
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POLYACRYLAMIDE gel electrophoresis , *MEMBRANE proteins , *VIBRIO parahaemolyticus , *GEL electrophoresis , *MOLECULAR weights , *SODIUM dodecyl sulfate - Abstract
Aims: The bacterium Vibrio parahaemolyticus causes gastroenteritis in humans after the consumption of raw seafood. Therefore, a rapid detection system that can identify the presence of V. parahaemolyticus accurately is necessary. One potential detection system under development is based on antibodies. This study aimed to map the outer membrane protein profile of V. parahaemolyticus. Methods and Results: These outer membrane proteins (OMP) may be antigenic and can be used to produce antibodies. The sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) method was used to map the outer membrane protein profile of V. parahaemolyticus. The results of the analysis using SDS-PAGE to assess the OMP of V. parahaemolyticus show that the molecular weight (MW) of the major polypeptide band was in the range of 10-100 kDa. Overall, there were 31 bands observed, of which 11 were major bands with MW of 72, 67, 58, 44, 42, 40, 38, 37, and 10 kDa. Conclusion, Significance, and Impact of Study: The identified proteins have antigenic properties and can be used to produce antibodies for a rapid detection system (rapid test) to detect the presence of V. parahaemolyticus. [ABSTRACT FROM AUTHOR]
- Published
- 2019