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1. Incorporation of disulfide containing protein modules into multivalent antigenic conjugates: generation of antibodies against the thrombin-sensitive region of murine protein S.

Author

Van de Vijver P, Schmitt M, Suylen D, Scheer L, Thomassen MC, Schurgers LJ, Griffin JH, Koenen RR, and Hackeng TM

Subjects
Animals, Antigens chemistry, Mice, Models, Molecular, Peptides chemical synthesis, Peptides chemistry, Protein Conformation, Antibodies immunology, Antigens immunology, Disulfides chemistry, Peptides immunology, Protein S chemistry, Protein S immunology, Thrombin metabolism
Abstract

Antigenic peptide conjugates can be used as vaccines and for the production of antibodies for clinical and research use. A method is presented here for the construction of conjugates incorporating oxidatively folded protein domains in their native conformation. This method was used to prepare multiple antigenic peptide constructs of the thrombin-sensitive loop region of murine anticoagulant protein S.

Published
2012
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