1. Development and evaluation of indirect enzyme-linked immunosorbent assay for a screening test to detect antibodies against classical swine fever virus.
- Author
-
Sakoda Y, Wakamoto H, Tamura T, Nomura T, Naito M, Aoki H, Morita H, Kida H, and Fukusho A
- Subjects
- Animals, Border Disease blood, Border Disease diagnosis, Border Disease immunology, Border disease virus immunology, Bovine Virus Diarrhea-Mucosal Disease blood, Bovine Virus Diarrhea-Mucosal Disease diagnosis, Bovine Virus Diarrhea-Mucosal Disease immunology, Cattle, Cell Line, Classical Swine Fever blood, Classical Swine Fever immunology, Classical Swine Fever Virus genetics, Classical Swine Fever Virus immunology, Diarrhea Viruses, Bovine Viral immunology, Enzyme-Linked Immunosorbent Assay veterinary, Neutralization Tests veterinary, Sensitivity and Specificity, Swine, Viral Envelope Proteins analysis, Viral Envelope Proteins genetics, Viral Envelope Proteins immunology, Antibodies, Viral blood, Classical Swine Fever diagnosis, Classical Swine Fever Virus isolation & purification, Enzyme-Linked Immunosorbent Assay methods
- Abstract
An indirect enzyme-linked immunosorbent assay (ELISA) was developed for a screening test to detect antibodies against classical swine fever virus (CSFV). Viral glycoproteins, which were purified from swine kidney cells infected with CSFV ALD/A76 strain by the immunoaffinity purification using monoclonal antibody against E2 protein, were adsorbed on a microtiter plate as the antigen for the antibody detection. Each antibody titer of serum sample was expressed as a sample per positive value calculated with optical absorbance of each sample and that of a positive control. The advantage of this ELISA is its higher sensitivity: most sera containing more than 4 neutralization titers were determined to be positive. This ELISA is unable to discriminate between antibodies against CSFV and those against other ruminant pestiviruses, therefore positive sera in this ELISA should be evaluated by a cross-neutralization test using CSFV, bovine viral diarrhea virus, and border disease virus. Taken together, the indirect ELISA developed in this study is useful screening tool to detect antibodies against CSFV for the large-scale monitoring of classical swine fever.
- Published
- 2012