1. The 4H84 monoclonal antibody detecting beta2m free nonclassical HLA-G molecules also binds to free heavy chains of classical HLA class I antigens present on activated lymphocytes.
- Author
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Poláková K, Kuba D, and Russ G
- Subjects
- Antibodies, Monoclonal metabolism, Antigens, CD analysis, Antigens, CD immunology, Antigens, Differentiation, B-Lymphocyte analysis, Antigens, Differentiation, B-Lymphocyte immunology, Cell Line, Tumor, Cross Reactions immunology, Flow Cytometry, Gene Expression drug effects, HLA Antigens genetics, HLA Antigens immunology, HLA-G Antigens, Histocompatibility Antigens Class I genetics, Histocompatibility Antigens Class I metabolism, Humans, Hydrogen-Ion Concentration, Leukocyte Common Antigens analysis, Leukocyte Common Antigens immunology, Leukocytes, Mononuclear drug effects, Leukocytes, Mononuclear immunology, Leukocytes, Mononuclear metabolism, Lymphocyte Activation immunology, Lymphocytes drug effects, Lymphocytes metabolism, Phytohemagglutinins pharmacology, Receptors, Transferrin, Reverse Transcriptase Polymerase Chain Reaction, beta 2-Microglobulin chemistry, Antibodies, Monoclonal immunology, HLA Antigens analysis, Histocompatibility Antigens Class I analysis, Histocompatibility Antigens Class I immunology, Lymphocytes immunology, beta 2-Microglobulin immunology
- Abstract
We have found that 4H84 monoclonal antibody (mAb) used for detection of beta2m free HLA-G molecules also binds to free heavy chains of classical HLA class I antigens generated on the cell surface by mild acid treatment. Here we demonstrate that beta2m free classical HLA class I molecules induced on the surface of activated lymphocytes not expressing HLA-G also bind 4H84 mAb. These results demonstrate that 4H84 mAb should be used for detection of HLA-G in cells and tissues with backing by other HLA-G specific mAbs.
- Published
- 2004
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