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Your search keyword '"Khrapova Np"' showing total 5 results
Start Over Author "Khrapova Np" Topic antibodies, monoclonal
5 results on '"Khrapova Np"'

1. [The isolation of monoclonal antibodies to Cryptococcus neoformans antigens].

Author

Khrapova NP, Lesovoĭ VS, Prokhvatilova EV, Stupenko LN, and Zharkova SF

Subjects
Animals, Antibody Specificity, Antigens, Surface immunology, Cryptococcus neoformans growth & development, Culture Media, Epitopes immunology, Hybridomas immunology, Immunization methods, Mice, Mice, Inbred BALB C, Tumor Cells, Cultured, Antibodies, Fungal isolation & purification, Antibodies, Monoclonal isolation & purification, Antigens, Fungal immunology, Cryptococcus neoformans immunology
Abstract

In this work conditions for the reproduction of hybridoma technology, specially adapted to C. neoformans, for obtaining monoclonal hybridomas (McAb) to diagnostically significant antigens of C. neoformans, the infective agent of cryptococcosis, are presented. The advantages of using the short-time cycle of stimulation of mouse B lymphocytes with low doses of C. neoformans capsular polysaccharide and the effectiveness of the hybridization of mouse spleen cells with myeloma cells, line Sp2/0, are shown. Four lines of stable hybridomas, producing McAb to different epitopes of C. neoformans surface antigens, have been obtained. The specific activity of McAb has been studied in the indirect immunofluorescence assay, the cytochemical and solid-phase enzyme immunoassays (EIA). McAb 3E2, Cr2 and 2G9 have been shown to be suitable for use in diagnostic EIA systems.

Published
1999

2. [The enzyme-antienzyme interactions of a number of Burkholderia pseudomallei hydrolases with monoclonal immunoglobulins].

Author

Tikhonov NG, Narbutovich NI, Khrapova NP, and Prokhvatilova EV

Subjects
Animals, Burkholderia pseudomallei immunology, Burkholderia pseudomallei pathogenicity, Drug Interactions, Hybridomas immunology, Hydrolases antagonists & inhibitors, Hydrolases drug effects, Mice, Mice, Inbred BALB C, Antibodies, Monoclonal pharmacology, Burkholderia pseudomallei enzymology, Enzyme Inhibitors pharmacology, Hydrolases pharmacology, Immunoglobulins pharmacology
Abstract

Phosphatase, phospholipase C and a proteolytic complex, including casein- and hemoglobin-hydrolases, have been isolated from cell-free extracts of B. pseudomallei cultivation medium. A set of monoclonal antibodies (McAb) to the antigenic complexes of this infective agent has been obtained. Conditions for the study of the interaction of the enzymes and McAb have been worked out, and most of the 14 immunoglobulins under study have been shown to neutralize 2-3 hydrolases each. Three types of McAb, selectively inhibiting only the proteolytic complex, only casein-hydrolase and only phospholipase C, have been identified.

Published
1999

3. [Monoclonal antibodies in the differentiation of Pseudomonas mallei and Pseudomonas pseudomallei].

Author

Iakovleva IV, Sviridov VV, Titova NG, Khrapova NP, Lipnitskiĭ AV, Farber SM, and Barkov AM

Subjects
Antibody Specificity, Antigens, Bacterial immunology, Bacteriological Techniques, Burkholderia pseudomallei pathogenicity, Immunologic Techniques, Pseudomonas pathogenicity, Virulence immunology, Antibodies, Bacterial analysis, Antibodies, Monoclonal analysis, Burkholderia pseudomallei immunology, Pseudomonas immunology
Published
1995

4. [The production of hybridomas producing monoclonal antibodies to the causative agent of melioidosis by using antigen-stimulated lymphocytes in an in-vitro system].

Author

Titova NG, Razina IV, Sviridov VV, Iakovleva IV, Khrapova NP, and Kulakov MIa

Subjects
Animals, Antibodies, Monoclonal analysis, Antibody Specificity, Immunization methods, Melioidosis immunology, Mice, Mice, Inbred BALB C, Antibodies, Monoclonal biosynthesis, Antigens, Bacterial immunology, Burkholderia pseudomallei immunology, Hybridomas immunology, Lymphocyte Activation immunology
Published
1995

5. [The outlook for improving the immunoglobulin preparations for the detection and identification of the causative agents of glanders and melioidosis].

Author

Khrapova NP, Tikhonov NG, Prokhvatilova EV, and Kulakov MIa

Subjects
Agglutination Tests methods, Agglutination Tests standards, Agglutination Tests statistics & numerical data, Animals, Burkholderia pseudomallei immunology, Hybridomas immunology, Immunodiffusion methods, Immunodiffusion standards, Immunodiffusion statistics & numerical data, Indicators and Reagents standards, Mice, Mice, Inbred BALB C, Pseudomonas immunology, Antibodies, Monoclonal isolation & purification, Burkholderia pseudomallei isolation & purification, Immunoglobulins isolation & purification, Pseudomonas isolation & purification
Abstract

The paper summarizes the data concerning the production and study of monoclonal antibodies (MAb) to the diagnostically significant glanders and melioidosis bacillus antigens. It evaluates the efficiency of using MAb in the gel immunodiffusion and agglutination tests as a basis of new-generation preparations for fluorescent antibody assay, indirect hemagglutination test which are used while detecting and identifying pathogenic pseudomonads. The paper defines the quality indices for monoclonal luminescent immunoglobulins and provides evidence for the benefits of monoclonal diagnostic agents over polyclonal analogues.

Published
1995

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