1. Immunological reactivity of blood from healthy humans to the rAls3p-N vaccine protein.
- Author
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Baquir B, Lin L, Ibrahim AS, Fu Y, Avanesian V, Tu A, Edwards J Jr, and Spellberg B
- Subjects
- Candidiasis immunology, Cytokines metabolism, Enzyme-Linked Immunosorbent Assay, Humans, Immunodominant Epitopes, Immunoglobulin A blood, Immunoglobulin E blood, Immunoglobulin G blood, Antibodies, Fungal blood, Candida immunology, Candidiasis prevention & control, Fungal Proteins immunology, Fungal Vaccines immunology
- Abstract
We determined reactivity of human blood to a vaccine based on the recombinant N-terminus of candidal Als3p (rAls3p-N) in preparation for future clinical trials. Healthy donor plasma had high immunoglobulin G titers (median, 1:51,200) and lower immunoglobulin A (median, 1:3,200) and immunoglobulin E (median, 1:128) titers to rAls3p-N by enzyme-linked immunosorbent assay. rAls3p-N stimulated interferon gamma (IFN-gamma) and interleukin (IL)-17, but not IL-4, from donor lymphocytes by enzyme-linked immunosorbent spot assay and IL-12 p70, IFN-gamma, IL-17, and IL-10 by cytometric bead array. Donors reacted to diverse immunodominant epitopes. Thus, facile humoral and cellular assays can monitor immune responses to the rAls3p-N vaccine in planned clinical trials. more...
- Published
- 2010
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