1. Clinical Evaluation of the Microscopic Observation Drug Susceptibility Assay for Detection of Mycobacterium tuberculosis Resistance to Isoniazid or Rifampin▿
- Author
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Mayra S. Arias, Carlos Leonardo Carvalho Pessôa, Richard E. Chaisson, Leila de Souza Fonseca, Mônica Kramer de Noronha Andrade, Afranio Lineu Kritski, Senia Rosales, Anna Grazia Marsico, Ada Pavón, Carlos Alvarado-Gálvez, Michael E. Kimerling, Fernanda Carvalho de Queiroz Mello, Susan E. Dorman, and Melly Pérez
- Subjects
Microbiology (medical) ,medicine.medical_specialty ,medicine.drug_class ,Antibiotics ,Antitubercular Agents ,Drug resistance ,Microbial Sensitivity Tests ,Biology ,Pharmacology ,Gastroenterology ,Mycobacterium tuberculosis ,Internal medicine ,Drug Resistance, Bacterial ,medicine ,Isoniazid ,Humans ,Prospective Studies ,Antibacterial agent ,Mycobacteriology and Aerobic Actinomycetes ,Assay sensitivity ,biology.organism_classification ,Confidence interval ,Rifampin ,Rifampicin ,medicine.drug - Abstract
This prospective study evaluated the performance of the m icroscopic o bservation d rug s usceptibility (MODS) assay for the direct detection of Mycobacterium tuberculosis drug resistance. MODS assay sensitivity, specificity, and positive and negative predictive values were 96.7% (95% confidence interval [95% CI], 92.1 to 98.8%), 78.4% (95% CI, 73.5 to 80.6%), 82.4% (95% CI, 78.4 to 84.2%), and 95.8% (95% CI, 89.9 to 98.5%), respectively, for isoniazid resistance and 96.0% (95% CI, 90.3 to 98.6%), 82.9% (95% CI, 78.8 to 84.7%), 80.0% (95% CI, 75.2 to 82.1%), and 96.7% (95% CI, 91.9 to 98.8%), respectively, for rifampin resistance. For both rifampin and isoniazid testing, the likelihood ratio for a negative test was ≤0.05, indicating that the MODS assay may be useful for ruling out drug resistance.
- Published
- 2007