1. In vivo and in vitro anti-inflammatory activity of neorogioltriol, a new diterpene extracted from the red algae Laurencia glandulifera.
- Author
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Chatter R, Othman RB, Rabhi S, Kladi M, Tarhouni S, Vagias C, Roussis V, Guizani-Tabbane L, and Kharrat R
- Subjects
- Animals, Anti-Inflammatory Agents isolation & purification, Anti-Inflammatory Agents metabolism, Anti-Inflammatory Agents toxicity, Anti-Inflammatory Agents, Non-Steroidal isolation & purification, Anti-Inflammatory Agents, Non-Steroidal metabolism, Anti-Inflammatory Agents, Non-Steroidal toxicity, Arthritis drug therapy, Aspirin pharmacology, Cell Survival drug effects, Control Groups, Dexamethasone pharmacology, Disease Models, Animal, Diterpenes isolation & purification, Diterpenes metabolism, Diterpenes toxicity, Dose-Response Relationship, Drug, Edema chemically induced, Inflammation Mediators antagonists & inhibitors, Inflammation Mediators physiology, Lipopolysaccharides metabolism, Lipopolysaccharides physiology, Macrophages drug effects, Macrophages metabolism, Mice, Phytotherapy, Plant Extracts isolation & purification, Plant Extracts metabolism, Plant Extracts toxicity, Rats, Time Factors, Anti-Inflammatory Agents pharmacology, Anti-Inflammatory Agents, Non-Steroidal pharmacology, Diterpenes pharmacology, Edema drug therapy, Laurencia chemistry, Plant Extracts pharmacology
- Abstract
Neorogioltriol is a tricyclic brominated diterpenoid isolated from the organic extract of the red algae Laurencia glandulifera. In the present study, the anti-inflammatory effects of neorogioltriol were evaluated both in vivo using carrageenan-induced paw edema and in vitro on lipopolysaccharide (LPS)-treated Raw264.7 macrophages. The in vivo study demonstrated that the administration of 1 mg/kg of neorogioltriol resulted in the significant reduction of carregeenan-induced rat edema. In vitro, our results show that neorogioltriol treatment decreased the luciferase activity in LPS-stimulated Raw264.7 cells, stably transfected with the NF-κB-dependent luciferase reporter. This effect on NF-κB activation is not mediated through MAPK pathways. The inhibition of NF-κB activity correlates with decreased levels of LPS-induced tumor necrosis factor-alpha (TNFα) present in neorogioltriol treated supernatant cell culture. Further analyses indicated that this product also significantly inhibited the release of nitric oxide and the expression of cyclooxygenase-2 (COX-2) in LPS-stimulated Raw264.7 cells. These latter effects could only be observed for neorogioltriol concentrations below 62.5 μM. To our knowledge, this is the first report describing a molecule derived from Laurencia glandulifera with anti-inflammatory activity both in vivo and in vitro. The effect demonstrated in vitro may be explained by the inhibition of the LPS-induced NF-κB activation and TNFα production. NO release and COX-2 expression may reinforce this effect.
- Published
- 2011
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