1. Characterization of a microbial polysaccharide-based bioflocculant and its anti-inflammatory and pro-coagulant activity.
- Author
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Zhong, Chunying, Cao, Gang, Rong, Kuan, Xia, Zhengwu, Peng, Ting, Chen, Honggao, and Zhou, Jiangang
- Subjects
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POLYSACCHARIDES , *FOURIER transform infrared spectroscopy , *X-ray photoelectron spectroscopy , *BIOCOMPATIBILITY , *NITRIC-oxide synthases , *INTERLEUKIN-6 , *TUMOR necrosis factors - Abstract
We describe a novel bioflocculant, MBF-15, which is an exopolysaccharide extracted from the alkaliphilic bacterium Paenibacillus jamilae. The biophysical characteristics of MBF-15 were determined using Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy. MBF-15 was also evaluated for its biocompatibility by examining its inflammatory, coagulant, and hemostatic properties in vitro and in vivo. Pretreatment of peripheral blood mononuclear cells with MBF-15 inhibited lipopolysaccharide-stimulated expression of inducible nitric oxide synthase, production of nitric oxide, and secretion of pro-inflammatory cytokines, including tumor necrosis factor-α and interleukin-6. In addition, MBF-15 increased both mRNA and protein levels of the anti-inflammatory cytokines transforming growth factor-β and IL-10. The hemocompatibility of MBF-15 was investigated by measuring the hemolysis ratio and clotting times. MBF-15 had high pro-thrombogenic activity but was not hemolytic. In a rat model, MBF-15 showed superior hemostatic properties compared with chitosan. Thus, MBF-15 offers a promising combination of anti-inflammatory and pro-coagulant properties that may be useful for hemostasis in a variety of clinical settings. [ABSTRACT FROM AUTHOR]
- Published
- 2018
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