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Start Over Author "Vuotto ML" Topic anti-bacterial agents
5 results on '"Vuotto ML"'

1. Antibacterial and antioxidant activities in Sideritis italica (Miller) Greuter et Burdet essential oils.

Author

Basile A, Senatore F, Gargano R, Sorbo S, Del Pezzo M, Lavitola A, Ritieni A, Bruno M, Spatuzzi D, Rigano D, and Vuotto ML

Subjects
Anti-Bacterial Agents isolation & purification, Antioxidants isolation & purification, Cells, Cultured, Dose-Response Relationship, Drug, Helicobacter pylori drug effects, Humans, Luminescent Measurements, Microbial Sensitivity Tests, Neutrophils drug effects, Oils, Volatile isolation & purification, Oils, Volatile pharmacology, Plant Components, Aerial chemistry, Plant Oils isolation & purification, Reactive Oxygen Species metabolism, Anti-Bacterial Agents pharmacology, Antioxidants pharmacology, Gram-Negative Bacteria drug effects, Gram-Positive Bacteria drug effects, Leukocytes drug effects, Plant Oils pharmacology, Sideritis chemistry
Abstract

Sideritis italica (Miller) Greuter et Burdet is a widespread Lamiacea in the Mediterranean region used in traditional medicine. Essential oils were antibacterial against nine ATCC and as many clinically isolated Gram-positive and Gram-negative bacterial strains. Antibacterial activity was also found against Helicobacter pylori: a dose-dependant inhibition was shown between 5 and 25 microg/ml. The antibacterial activity of the oils was expressed as MICs (minimum inhibitory concentrations) and MBCs (minimum bactericidal concentrations). At a concentration between 3.9 and 250 microg/ml the oils showed a significant antibacterial effect against both Gram-negative and Gram-positive bacteria. In particular the ATCC strains Pseudomonas aeruginosa (MIC=3.9 microg/ml and 7.8 for flowerheads and leaves, respectively), Proteus mirabilis (MIC=15.6 and 7.8 microg/ml), Salmonella typhi (MIC=7.8 microg/ml) and Proteus vulgaris (MIC=15.6 microg/ml) were the most inhibited. Only Pseudomonas aeruginosa showed MBC at a concentration between 62.6 and 125 microg/ml. The antioxidant activity of the essential oils was evaluated by two cell free colorimetric methods: ABTS and DMPD; leaf oil is more active (4.29 +/- 0.02 trolox equivalents and 4.53 +/- 0.67 ascorbic acid equivalents by ABTS and DMPD, respectively). Finally the antioxidant activity of the essential oils was also evaluated by their effects on human whole blood leukocytes (WB) and on isolated polymorphonucleate (PMN) chemiluminescence. Comparing the effects of the oils from leaves and flowerheads on both PMN and WB chemiluminescence emission, we found no significant differences. Essential oils showed a dose-dependent and linear inhibitory activity on isolated PMN as well as on WB CL emission when PMA-stimulated. On the contrary, the inhibitory activity on resting cells was nonlinear. Our data represent an answer to the continual demand for new antibiotics and antioxidants for the continuous emergence of antibiotic-resistant strains and the growing interest in the substitution of synthetic antioxidants with natural ones. Furthermore, our finding of antibacterial and antioxidant activities in the essential oils from Sideritis italica flowerheads and leaves validated the use of the plant for medical purposes.

Published
2006
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2. Antibacterial and allelopathic activity of extract from Castanea sativa leaves.

Author

Basile A, Sorbo S, Giordano S, Ricciardi L, Ferrara S, Montesano D, Castaldo Cobianchi R, Vuotto ML, and Ferrara L

Subjects
Anti-Bacterial Agents chemistry, Anti-Bacterial Agents therapeutic use, Chromatography, High Pressure Liquid, Chromatography, Thin Layer, Flavonoids chemistry, Humans, Microbial Sensitivity Tests, Plant Extracts chemistry, Plant Extracts therapeutic use, Plant Leaves, Plant Roots, Raphanus, Anti-Bacterial Agents pharmacology, Fagaceae, Gram-Negative Bacteria drug effects, Gram-Positive Bacteria drug effects, Phytotherapy, Plant Extracts pharmacology
Abstract

Following the extraction of Castanea sativa with an aqueous solution of sulfuric acid (pH 3.0), the ethyl acetate soluble fraction was tested for its antibacterial and allelopathic activity. The extract was shown to have pronounced antibacterial effects against seven of the eight strains of Gram-positive and Gram-negative bacteria used (MIC in the range of 64-256 microg/ml and MBC in the range of 256-512 microg/ml). The active fraction was analyzed by TLC and HPLC showing the presence of rutin, hesperidin, quercetin, apigenin, morin, naringin, galangin and kaempferol. Standards of the identified flavonoids were tested against the same bacterial strains. The highest activity was shown by quercetin, rutin and apigenin. The allelopathic effect was tested against Raphanus sativus seed germination. The extract, quercetin, rutin and apigenin caused a decrease in the percentage of seed germination and root and epicotyl growth.

Published
2000
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3. Antimicrobial and antioxidant activities of Feijoa sellowiana fruit.

Author

Vuotto ML, Basile A, Moscatiello V, De Sole P, Castaldo-Cobianchi R, Laghi E, and Ielpo MT

Subjects
Anti-Bacterial Agents chemistry, Antioxidants chemistry, Enterobacter drug effects, Humans, In Vitro Techniques, Indicators and Reagents, Luminescent Measurements, Luminol, Microbial Sensitivity Tests, Neutrophils drug effects, Plant Extracts chemistry, Plant Extracts pharmacology, Pseudomonas drug effects, Staphylococcus drug effects, Streptococcus drug effects, Anti-Bacterial Agents pharmacology, Antioxidants pharmacology, Fruit chemistry, Plants, Medicinal chemistry
Abstract

The present study was designed to evaluate the antibacterial and antioxidant activities of an aqueous extract from the tropical Feijoa sellowiana Berg. fruit which is widely used for human food. The extract was tested against gram-positive and gram-negative bacteria by a broth dilution test and on human whole blood leukocytes, as well as isolated neutrophils using a chemiluminescence (CL) assay. The extract inhibited bacterial growth; Pseudomonas aeruginosa, Enterobacter aerogenes and Enterobacter cloacae were the most sensitive. The fruit extract significantly decreased CL emission from human whole blood phagocytes and isolated polymorphonuclear leukocytes whether they were activated or not by soluble or phagocytic stimuli. F. sellowiana showed both antibacterial and antioxidant properties and therefore its extract might be used as a new multifaceted drug.

Published
2000
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4. Antibacterial and antioxidant activities in Sideritis italica (Miller) Greuter et Burdet essential oils

Author

Alfredo Lavitola, Maurizio Bruno, Sergio Sorbo, Rosalba Gargano, Marisa Del Pezzo, Adriana Basile, Alberto Ritieni, Daniela Spatuzzi, Felice Senatore, Daniela Rigano, Maria Luisa Vuotto, BASILE, A, SENATORE, F, GARGANO, R, SORBO, S, DEL PEZZO, M, LAVITOLA, A, RITIENI, A, BRUNO, M, SPATUZZI, D, RIGANO, D, VUOTTO, ML, Basile, Adriana, Senatore, Felice, R., Gargano, Sorbo, Sergio, M., Del Pezzo, Lavitola, Alfredo, Ritieni, Alberto, M., Bruno, D., Spatuzzi, Rigano, Daniela, and M. L., Vuotto

Subjects
Neutrophils, Proteus vulgaris, antioxidant activity, Microbial Sensitivity Tests, Gram-Positive Bacteria, Antioxidants, essential oil, Microbiology, law.invention, chemistry.chemical_compound, antibacterial activity, law, Drug Discovery, Gram-Negative Bacteria, Leukocytes, Oils, Volatile, Sideritis italica, Humans, Plant Oils, Food science, Essential oil, Cells, Cultured, Antibacterial agent, Pharmacology, ABTS, biology, Dose-Response Relationship, Drug, Helicobacter pylori, Plant Components, Aerial, biology.organism_classification, Ascorbic acid, Anti-Bacterial Agents, chemistry, Luminescent Measurements, Sideritis, Trolox, Antibacterial activity, Reactive Oxygen Species
Abstract

Sideritis italica (Miller) Greuter et Burdet is a widespread Lamiacea in the Mediterranean region used in traditional medicine. Essential oils were antibacterial against nine ATCC and as many clinically isolated Gram-positive and Gram-negative bacterial strains. Antibacterial activity was also found against Helicobacter pylori: a dose-dependant inhibition was shown between 5 and 25 microg/ml. The antibacterial activity of the oils was expressed as MICs (minimum inhibitory concentrations) and MBCs (minimum bactericidal concentrations). At a concentration between 3.9 and 250 microg/ml the oils showed a significant antibacterial effect against both Gram-negative and Gram-positive bacteria. In particular the ATCC strains Pseudomonas aeruginosa (MIC=3.9 microg/ml and 7.8 for flowerheads and leaves, respectively), Proteus mirabilis (MIC=15.6 and 7.8 microg/ml), Salmonella typhi (MIC=7.8 microg/ml) and Proteus vulgaris (MIC=15.6 microg/ml) were the most inhibited. Only Pseudomonas aeruginosa showed MBC at a concentration between 62.6 and 125 microg/ml. The antioxidant activity of the essential oils was evaluated by two cell free colorimetric methods: ABTS and DMPD; leaf oil is more active (4.29 +/- 0.02 trolox equivalents and 4.53 +/- 0.67 ascorbic acid equivalents by ABTS and DMPD, respectively). Finally the antioxidant activity of the essential oils was also evaluated by their effects on human whole blood leukocytes (WB) and on isolated polymorphonucleate (PMN) chemiluminescence. Comparing the effects of the oils from leaves and flowerheads on both PMN and WB chemiluminescence emission, we found no significant differences. Essential oils showed a dose-dependent and linear inhibitory activity on isolated PMN as well as on WB CL emission when PMA-stimulated. On the contrary, the inhibitory activity on resting cells was nonlinear. Our data represent an answer to the continual demand for new antibiotics and antioxidants for the continuous emergence of antibiotic-resistant strains and the growing interest in the substitution of synthetic antioxidants with natural ones. Furthermore, our finding of antibacterial and antioxidant activities in the essential oils from Sideritis italica flowerheads and leaves validated the use of the plant for medical purposes.

Published
2006

5. ANTIMICROBIAL AND ANTIOXIDANT ACTIVITIES OF FEIJOA SELLOWIANA FRUIT

Author

Maria Teresa Lucia Ielpo, V. Moscatiello, Emanuela Laghi, R. Castaldo-Cobianchi, Adriana Basile, Pasquale De Sole, Maria Luisa Vuotto, Vuotto, Ml, Basile, Adriana, Moscatiello, V, DE SOLE, P, CASTALDO COBIANCHI, R, Laghi, E, and Ielpo, Mtl

Subjects
Microbiology (medical), Antioxidant, Neutrophils, Staphylococcus, Gram-positive bacteria, medicine.medical_treatment, Enterobacter, Microbial Sensitivity Tests, In Vitro Techniques, Pharmacognosy, Enterobacter aerogenes, Antioxidants, Microbiology, Pseudomonas, medicine, Humans, Pharmacology (medical), Food science, Antibacterial agent, Plants, Medicinal, biology, Plant Extracts, Streptococcus, General Medicine, biology.organism_classification, Antimicrobial, Anti-Bacterial Agents, Infectious Diseases, Fruit, Luminescent Measurements, Indicators and Reagents, Luminol, Antibacterial activity, Enterobacter cloacae
Abstract

The present study was designed to evaluate the antibacterial and antioxidant activities of an aqueous extract from the tropical Feijoa sellowiana Berg. fruit which is widely used for human food. The extract was tested against gram-positive and gram-negative bacteria by a broth dilution test and on human whole blood leukocytes, as well as isolated neutrophils using a chemiluminescence (CL) assay. The extract inhibited bacterial growth; Pseudomonas aeruginosa, Enterobacter aerogenes and Enterobacter cloacae were the most sensitive. The fruit extract significantly decreased CL emission from human whole blood phagocytes and isolated polymorphonuclear leukocytes whether they were activated or not by soluble or phagocytic stimuli. F. sellowiana showed both antibacterial and antioxidant properties and therefore its extract might be used as a new multifaceted drug.

Published
1999

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