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Your search keyword '"Vuotto, M. L."' showing total 3 results
Start Over Author "Vuotto, M. L." Topic anti-bacterial agents
3 results on '"Vuotto, M. L."'

1. Antibacterial and allelopathic activity of extract from Castanea sativa leaves.

Author

Basile A, Sorbo S, Giordano S, Ricciardi L, Ferrara S, Montesano D, Castaldo Cobianchi R, Vuotto ML, and Ferrara L

Subjects
Anti-Bacterial Agents chemistry, Anti-Bacterial Agents therapeutic use, Chromatography, High Pressure Liquid, Chromatography, Thin Layer, Flavonoids chemistry, Humans, Microbial Sensitivity Tests, Plant Extracts chemistry, Plant Extracts therapeutic use, Plant Leaves, Plant Roots, Raphanus, Anti-Bacterial Agents pharmacology, Fagaceae, Gram-Negative Bacteria drug effects, Gram-Positive Bacteria drug effects, Phytotherapy, Plant Extracts pharmacology
Abstract

Following the extraction of Castanea sativa with an aqueous solution of sulfuric acid (pH 3.0), the ethyl acetate soluble fraction was tested for its antibacterial and allelopathic activity. The extract was shown to have pronounced antibacterial effects against seven of the eight strains of Gram-positive and Gram-negative bacteria used (MIC in the range of 64-256 microg/ml and MBC in the range of 256-512 microg/ml). The active fraction was analyzed by TLC and HPLC showing the presence of rutin, hesperidin, quercetin, apigenin, morin, naringin, galangin and kaempferol. Standards of the identified flavonoids were tested against the same bacterial strains. The highest activity was shown by quercetin, rutin and apigenin. The allelopathic effect was tested against Raphanus sativus seed germination. The extract, quercetin, rutin and apigenin caused a decrease in the percentage of seed germination and root and epicotyl growth.

Published
2000
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2. Antimicrobial and antioxidant activities of Feijoa sellowiana fruit.

Author

Vuotto ML, Basile A, Moscatiello V, De Sole P, Castaldo-Cobianchi R, Laghi E, and Ielpo MT

Subjects
Anti-Bacterial Agents chemistry, Antioxidants chemistry, Enterobacter drug effects, Humans, In Vitro Techniques, Indicators and Reagents, Luminescent Measurements, Luminol, Microbial Sensitivity Tests, Neutrophils drug effects, Plant Extracts chemistry, Plant Extracts pharmacology, Pseudomonas drug effects, Staphylococcus drug effects, Streptococcus drug effects, Anti-Bacterial Agents pharmacology, Antioxidants pharmacology, Fruit chemistry, Plants, Medicinal chemistry
Abstract

The present study was designed to evaluate the antibacterial and antioxidant activities of an aqueous extract from the tropical Feijoa sellowiana Berg. fruit which is widely used for human food. The extract was tested against gram-positive and gram-negative bacteria by a broth dilution test and on human whole blood leukocytes, as well as isolated neutrophils using a chemiluminescence (CL) assay. The extract inhibited bacterial growth; Pseudomonas aeruginosa, Enterobacter aerogenes and Enterobacter cloacae were the most sensitive. The fruit extract significantly decreased CL emission from human whole blood phagocytes and isolated polymorphonuclear leukocytes whether they were activated or not by soluble or phagocytic stimuli. F. sellowiana showed both antibacterial and antioxidant properties and therefore its extract might be used as a new multifaceted drug.

Published
2000
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3. Antibacterial and allelopathic activity of extract from Castanea sativa leaves

Author

Domenico Montesano, Lydia Ferrara, L Ricciardi, Adriana Basile, Simonetta Giordano, Maria Luisa Vuotto, S. Ferrara, R. Castaldo Cobianchi, Sergio Sorbo, Basile, A., Sorbo, S., Giordano, S., Ricciardi, L., Ferrara, S., Montesano, D., CASTALDO COBIANCHI, R., Vuotto, M. L., and Ferrara, Lydia

Subjects
Microbial Sensitivity Tests, Fagaceae, Gram-Positive Bacteria, Plant Roots, Raphanus, Rutin, chemistry.chemical_compound, Drug Discovery, Botany, Gram-Negative Bacteria, Humans, Food science, Naringin, Chromatography, High Pressure Liquid, Pharmacology, Flavonoids, Plant Extracts, food and beverages, General Medicine, Anti-Bacterial Agents, Galangin, Plant Leaves, chemistry, Apigenin, Epicotyl, Chromatography, Thin Layer, Kaempferol, Antibacterial activity, Quercetin, Phytotherapy
Abstract

Following the extraction of Castanea sativa with an aqueous solution of sulfuric acid (pH 3.0), the ethyl acetate soluble fraction was tested for its antibacterial and allelopathic activity. The extract was shown to have pronounced antibacterial effects against seven of the eight strains of Gram-positive and Gram-negative bacteria used (MIC in the range of 64-256 microg/ml and MBC in the range of 256-512 microg/ml). The active fraction was analyzed by TLC and HPLC showing the presence of rutin, hesperidin, quercetin, apigenin, morin, naringin, galangin and kaempferol. Standards of the identified flavonoids were tested against the same bacterial strains. The highest activity was shown by quercetin, rutin and apigenin. The allelopathic effect was tested against Raphanus sativus seed germination. The extract, quercetin, rutin and apigenin caused a decrease in the percentage of seed germination and root and epicotyl growth.

Published
2000

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