1. Biosynthesis of Polyhydroalkanoates Doped with Silver Nanoparticles Using Pseudomonas putida and Pseudomonas aeruginosa for Antibacterial Polymer Applications.
- Author
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Cruz-Romero CL, Chávez-Ramírez AU, Flores-Juárez CR, Arjona N, Álvarez-López A, Del Bosque Plata L, Vallejo-Becerra V, and Galindo-de-la-Rosa JD
- Subjects
- Microbial Sensitivity Tests, Pseudomonas aeruginosa drug effects, Pseudomonas aeruginosa metabolism, Pseudomonas putida metabolism, Silver chemistry, Silver pharmacology, Polyhydroxyalkanoates biosynthesis, Polyhydroxyalkanoates chemistry, Metal Nanoparticles chemistry, Anti-Bacterial Agents pharmacology, Anti-Bacterial Agents chemistry, Anti-Bacterial Agents biosynthesis, Glycerol chemistry, Glycerol metabolism
- Abstract
In this study, the biosynthesis of polyhydroxyalkanoates (PHAs) was carried out using Pseudomonas putida and Pseudomonas aeruginosa . These PHAs were produced using reagent-grade glycerol and crude glycerol as the carbon sources. The objective was to compare the production of PHAs and to functionalize these polymers with silver nanoparticles to provide antibacterial properties for potential biomedical applications. The findings from the physical and chemical analyses confirmed the successful synthesis and extraction of PHAs, achieving comparable yields using both crude glycerol and reagent-grade glycerol as carbon sources across both strains. Approximately 16% higher PHAs production was obtained using Pseudomonas putida compared to Pseudomonas aeruginosa , and no significant difference was observed in the production rate of PHAs between the two carbon sources used, which means that crude glycerol could be utilized even though it has more impurities. Notably, PHAs functionalized with silver nanoparticles showed improved antibacterial effectiveness, especially those derived from reagent-grade glycerol and the Pseudomonas aeruginosa strain.
- Published
- 2024
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