1. Prevalence of β-lactamase genes in domestic washing machines and dishwashers and the impact of laundering processes on antibiotic-resistant bacteria.
- Author
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Rehberg L, Frontzek A, Melhus Å, and Bockmühl DP
- Subjects
- Bacteria drug effects, Bacteria genetics, Bacteria isolation & purification, Bacterial Proteins genetics, Bacterial Proteins metabolism, Drug Resistance, Bacterial, Household Articles statistics & numerical data, Microbial Sensitivity Tests, Prevalence, beta-Lactamases metabolism, Anti-Bacterial Agents pharmacology, Bacteria enzymology, Equipment Contamination statistics & numerical data, Laundering instrumentation, beta-Lactamases genetics, beta-Lactams pharmacology
- Abstract
Aims: To investigate the prevalence of β-lactamase genes in domestic washing machines and dishwashers, and the decontamination efficacy of laundering., Methods and Results: For the first investigation, swab samples from washing machines (n = 29) and dishwashers (n = 24) were analysed by real-time quantitative PCR to detect genes encoding β-lactamases. To test the impact of laundering on resistant bacteria, cotton test swatches were artificially contaminated with susceptible and resistant strains of Pseudomonas aeruginosa, Klebsiella pneumoniae and Staphylococcus aureus within a second investigation. They were washed in a domestic washing machine with or without activated oxygen bleach (AOB)-containing detergent at 20-50°C. β-Lactamase genes (most commonly of the AmpC- and OXA-type) were detected in 79% of the washing machines and in 96% of the dishwashers and Pseudomonadaceae dominated the microbiota. The level of bacterial reduction after laundering was ≥80% for all Ps. aeruginosa and Kl. pneumoniae strains, while it was only 37-61% for the methicillin-resistant Staph. aureus outbreak strain. In general, the reduction was tendentially higher for susceptible bacteria than for the resistant outbreak strains, especially for Staph. aureus., Conclusions: β-Lactamase genes seem to be frequently present in domestic appliances and may pose a potential risk for cross-contamination and horizontal transfer of genes encoding resistance against clinically important β-lactams. In general, higher temperatures and the use of AOB can improve the reduction of antibiotic-resistant bacteria, including Staph. aureus which appears to be less susceptible to the decontamination effect of laundering., Significance and Impact of This Study: Data on the presence of antibiotic-resistant bacteria in the domestic environment are limited. This study suggests that β-lactamase genes in washing machines and dishwashers are frequent, and that antibiotic-resistant strains are generally more resistant to the used washing conditions., (© 2017 The Society for Applied Microbiology.)
- Published
- 2017
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