1. Miniaturization of the structure elucidation of novel natural products--two trace antibacterial acylated caprylic alcohol glycosides from Arctostaphylos pumila.
- Author
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Hu JF, Yoo HD, Williams CT, Garo E, Cremin PA, Zeng L, Vervoort HC, Lee CM, Hart SM, Goering MG, O'Neil-Johnson M, and Eldridge GR
- Subjects
- Anti-Bacterial Agents administration & dosage, Anti-Bacterial Agents chemistry, Anti-Bacterial Agents therapeutic use, Glycosides administration & dosage, Glycosides chemistry, Glycosides pharmacology, Glycosides therapeutic use, Humans, Methicillin Resistance, Microbial Sensitivity Tests, Plant Extracts administration & dosage, Plant Extracts chemistry, Plant Extracts therapeutic use, Anti-Bacterial Agents pharmacology, Arctostaphylos, Phytotherapy, Plant Extracts pharmacology, Staphylococcus aureus drug effects
- Abstract
High-throughput isolation, purification and analysis methods applied to natural products libraries from plants gave rise to the discovery of two novel acylated caprylic alcohol glycosides (1, 2) produced by Arctostaphylos pumila. The NMR spectra were acquired using the CapNMR probe and performed on mass-limited samples, which enabled us to elucidate the structures of 2,6-diacetyl-3,4-diisobutyl-1- O-octylglucopyranoside (1, 200 microg) and 2,6-diacetyl-3,4-dimethylbutyl-1- O-octylglucopyranosid (2, 70 microg). Compounds 1 and 2 exhibited antibacterial activity against Gram-positive methicillin-resistant Staphylococcus aureus with an MIC of 128 microg/mL and 64 microg/mL, respectively.
- Published
- 2005
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