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The purple tubers of Dioscorea alata L. have been found to contain a variety of bioactive chemical components, including anthocyanins, which make it significant to investigate the pre-protective effects of Dioscorea alata L. and its crude extracts on cells prior to oxidative stress. To establish a suitable oxidative damage model, an injured model of IPEC-J2 cells was created using H2O2 as the oxidant. Specifically, when the concentration of H2O2 was 120 μmol/L and the injured time was 8 h, the survival rate of cells decreased to approximately 70%, and the cells exhibited a noticeable oxidative stress reaction. Moreover, the crude extracts of Dioscorea alata L. demonstrated beneficial pre-protective effects on IPEC-J2 cells by increasing the total antioxidant capacity (T-AOC) and catalase (CAT) activities, augmenting the expression of total superoxide dismutase (T-SOD) and its genes, reducing the content of malondialdehyde (MDA) and the activity of glutathione peroxidase (GSH-PX) and its expression of genes, and promoting the expression of glucose transporter SGLT1 gene while reducing that of GULT2 gene, thereby facilitating the entry of anthocyanins into cells. In addition, the 50 μg/mL crude extracts effectively inhibited the phosphorylation of IκB and the p65 protein, thus reducing cellular oxidative stress. Given these findings, Dioscorea alata L. can be considered a natural antioxidant for practical breeding and production purposes, with an optimal concentration of crude extracts in this experiment being 50 μg/mL.

Dioscorea alata L. anthocyanins (DAC) are natural compounds found in plants and have shown potential health benefits. The objective of this investigation was to assess the impact of anthocyanins sourced from Dioscorea alata L. on the growth, carcass traits, antioxidant potential, and immune response of Hainan black goats. In this study, 30 three-month-old Hainan black goats (with a weight of 11.30 ± 0.82 kg) were selected and randomly divided into two groups, with 15 goats in each group. During the 60-day experiment, the control group (CON) and the treatment group (DAC) were, respectively, supplemented with 0 and 40 mg/kg BW of DAC in the basal diet. The results showed that DAC had no significant impact on the growth performance and body characteristics of Hainan black goats (p > 0.05). However, in terms of meat quality, the addition of DAC significantly increased the pH value and cooking yield 24 h post-slaughter (p < 0.05), while reducing the shear force of the meat (p < 0.05). Compared to the control group, adding DAC to the feed resulted in a significant increase in the total antioxidant capacity (T-AOC) and superoxide dismutase (T-SOD) concentrations in plasma after 30 days of feeding (p < 0.05). After 60 days of feeding, the concentrations of T-AOC, T-SOD, glutathione peroxidase (GSH-Px), and catalase (CAT) in the plasma of the DAC group was higher than that of the control group (p < 0.05), while the concentration of malondialdehyde (MDA) was lower than that of the control group (p < 0.05). In addition, supplementing DAC significantly increased the content of interleukin-10 (IL-10) and immunoglobulin M (IgM) in the plasma of Hainan black goats after 30 days of feeding (p < 0.05), while reducing the content of interleukin-6 (IL-6) (p < 0.05). After 60 days of feeding, the immunoglobulin G (IgG) and IL-10 content in the plasma of the DAC group was significantly increased (p < 0.05), while the concentrations of IL-1ß, IL-6, and tumor necrosis factor-a (TNF-a) were suppressed (p < 0.05). In summary, these results indicate that supplementing DAC can improve the meat quality, enhance the antioxidant capacity, and immune function of Hainan black goats. [ABSTRACT FROM AUTHOR]