1. Nanoencapsulated Melaleuca alternifolia essential oil exerts anesthetic effects in the brachyuran crab using Neohelice granulate
- Author
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Fábio Everton Maciel, Matheus D. Baldissera, Tábata Martins de Lima, Roberto Christ Vianna Santos, Renata Platcheck Raffin, Márcio Alberto Geihs, Berta Maria Heinzmann, Luiz Eduardo Maia Nery, Braulio Otomar Caron, Carine F. Souza, and Bernardo Baldisserotto
- Subjects
0301 basic medicine ,Male ,Brachyura ,invertebrate ,anesthesia ,law.invention ,03 medical and health sciences ,chemistry.chemical_compound ,Nanocapsules ,law ,medicine ,Oils, Volatile ,Animals ,lcsh:Science ,Neohelice ,Essential oil ,Anesthetics ,terpinen-4-ol ,Multidisciplinary ,Traditional medicine ,biology ,nanotechnology ,Plant Extracts ,Terpinen-4-ol ,Tea tree oil ,Melaleuca alternifolia ,04 agricultural and veterinary sciences ,biology.organism_classification ,Melaleuca ,humanities ,Eugenol ,030104 developmental biology ,chemistry ,Anesthetic ,040102 fisheries ,0401 agriculture, forestry, and fisheries ,lcsh:Q ,eugenol ,tea-tree-oil ,Lippia alba ,medicine.drug - Abstract
The aim of this study was to evaluate the efficacy and safety of several anesthetics in the brachyuran crab Neohelice granulata, an emergent experimental model. The essential oils (EOs) of Lippia alba, Aloysia tryphilla, and Melaleuca alternifolia (tea tree oil; TTO), the isolated compounds eugenol, menthol, terpinen-4-ol, and the nanoencapsulated form of TTO, were administered in one or more of the following ways: added to the water (immersion), through an arthrodial membrane (injected), or by oral gavage. Unexpectedly, most EOs did not produce an anesthetic effect after immersion. Only TTO and eugenol induced anesthesia by immersion, with very long induction and recovery times compared to anesthesia of other crustaceans. However, a good anesthetic effect was observed with the injection of terpinen-4-ol and nanoencapsulated TTO in N. granulata; both demonstrated ideal induction and recovery times. These substances appear to be promising anesthetic alternatives for crustaceans.
- Published
- 2018