1. [Effect of
- Author
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Qiao, Zhou, Hong-Wu, Yu, Yan, Zhu, Yu-Zhen, Pan, Jia, Yang, Bing-Kun, Wu, Yun-Yan, Cao, and Xue, Hu
- Subjects
Cartilage, Articular ,Male ,Hyperplasia ,Tumor Necrosis Factor-alpha ,Acupuncture Therapy ,Arthritis, Experimental ,Chromatin ,Interleukin-10 ,Rats ,Matrix Metalloproteinase 9 ,Animals ,Matrix Metalloproteinase 3 ,Interleukin-4 ,Rats, Wistar - Abstract
To observe the effect ofA total of 60 clean male Wistar rats were randomized into a normal group, a model group, a tripterygium wilfordii polyglycoside tablet (TWP) group and an acupuncture group, 15 rats in each group. Rats in the model group, the TWP group and the acupuncture group received intradermal injection of Freund's complete adjuvant (FCA) at right hind foot pad to induce the AA model. TWP suspension of 8 mg/kg was given by gavage in the TWP group. Acupuncture was applied at "Shenshu" (BL 23), "Pishu" (BL 20) and right "Housanli" (ST 36), "Sanyinjiao" (SP 6), "Yanglingquan" (GB 34) in the acupuncture group, 15 min a time, once a day. The intervention was given 15 days in both TWP group and acupuncture group. The foot-pad swelling degree before modeling, before and after intervention and the arthritis index (AI) score before and after intervention were calculated; the serum levels of interleukin (IL)-1β, IL-4, IL-10 and tumor necrosis factor-α (TNF-α) were detected by ELISA method; the ultrastructure and histomorphological changes of synovium issue were observed by transmission electron microscope and HE staining; the positive expression of matrix metalloproteinase (MMP)-3 and MMP-9 in synovium issue was detected by immunohistochemistry method.Before intervention, foot-pad swelling degree of the model group, the TWP group and the acupuncture group was increased compared with the normal group (
- Published
- 2022