1. Exploring the FL-160-CRP gene family through sequence variability of the complement regulatory protein (CRP) expressed by the trypomastigote stage of Trypanosoma cruzi
- Author
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Olivia Touzet, Ali Ouaissi, Favio Parrado, Marie-France Bosseno, Salima Fatha, Joël Lelièvre, Simone Frédérique Brenière, Ana M. Rojas, Françoise Mathieu-Daudé, Bénédicte Lafay, UR008 Pathogénie des Trypanosomatidés (IRD), Institut de Recherche pour le Développement (IRD [France-Sud]), Génétique et évolution des maladies infectieuses (GEMI), Université Montpellier 1 (UM1)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud]), Muscle et pathologies, Université Montpellier 1 (UM1)-IFR3, Université Montpellier 1 (UM1)-Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire de chimie de coordination (LCC), Institut de Chimie de Toulouse (ICT-FR 2599), Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut de Recherche pour le Développement (IRD)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS), UMA de Pneumologie, Centre Hospitalier du Val d?Ariège, Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Institut de Recherche pour le Développement (France), Institut National de la Santé et de la Recherche Médicale (France), Centre National de la Recherche Scientifique (CNRS)-Université Montpellier 1 (UM1)-Institut de Recherche pour le Développement (IRD [France-Sud]), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie de Toulouse (ICT-FR 2599), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Spanish National Cancer Research Center (CNIO), Dynamique des interactions membranaires normales et pathologiques (DIMNP), and Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
Amino Acid Motifs ,Genes, Protozoan ,MESH: Membrane Glycoproteins ,Protozoan Proteins ,MESH: Amino Acid Sequence ,MESH: Amino Acid Motifs ,0302 clinical medicine ,trans sialidase like proteins ,Cluster Analysis ,MESH: Animals ,MESH: Genetic Variation ,MESH: Phylogeny ,MESH: Protozoan Proteins ,Peptide sequence ,Phylogeny ,chemistry.chemical_classification ,Genetics ,0303 health sciences ,Membrane Glycoproteins ,Multiple sequence alignment ,MESH: Neuraminidase ,Genome project ,3. Good health ,Infectious Diseases ,MESH: Genes, Protozoan ,Multigene Family ,MESH: Trypanosoma cruzi ,Microbiology (medical) ,Trypanosoma cruzi ,Molecular Sequence Data ,030231 tropical medicine ,MESH: Glycoproteins ,Neuraminidase ,Biology ,Microbiology ,03 medical and health sciences ,FL 160 ,Phylogenetics ,Genetic variation ,Animals ,Gene family ,[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology ,Amino Acid Sequence ,MESH: Life Cycle Stages ,Molecular Biology ,Ecology, Evolution, Behavior and Systematics ,Glycoproteins ,030304 developmental biology ,Life Cycle Stages ,MESH: Molecular Sequence Data ,FL-160 ,Trans-sialidase-like proteins ,Genetic Variation ,[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology ,biology.organism_classification ,MESH: Cluster Analysis ,chemistry ,Complement regulatory protein ,MESH: Multigene Family ,[SDE.BE]Environmental Sciences/Biodiversity and Ecology ,Glycoprotein - Abstract
The complement regulatory protein (CRP) of Trypanosoma cruzi is a surface glycoprotein which confers to the infectious trypomastigote forms a protection against the lytic activity of the host complement. CRP belongs to the large family of the trans-sialidase-like proteins and its sequence is highly similar to those of the flagellar FL-160 and chronic exoantigen proteins, encoded by a multigene family. To further define the gene family encoding the CRP, we investigated the protein diversity among several strains of T. cruzi through the sequencing of trypomastigote transcripts, and used a phylogenetic analysis based on the multiple alignment of these proteins with the top scoring sequences detected by a database sequence homology search. Intrastrain variations in CRP sequences revealed the existence of several copies per strain. The interstrain variability of CRP was consistent with the genetic subdivisions of T. cruzi into lineages and discrete typing units. The phylogenetic analysis based on a 227 amino acid alignment of CRP sequences with the 200 putative proteins retrieved from the protein databases (including the sequences from the T. cruzi genome project) revealed that the CRP sequences clustered with the FL-160 proteins into a monophyletic group characterized by the presence of the 12 amino acid mimicry epitope that mimics nervous tissues. The phylogeny did not differentiate between the CRP and the FL-160 proteins. The identification of this group of CRP-like proteins and the high sequence similarity observed within it open up new prospects for the exploration of the localization, structure and function of these proteins and a better understanding of their involvement in key aspects of host–parasite interactions, such as the resistance to the complement. This work provides also information for the T. cruzi genome annotation of the trans-sialidase-like putative proteins., These investigations received financial support from the “Institut de Recherche pour le Développement” (IRD), the “Institut National de la Santé et de la Recherche Médicale” (INSERM), and the French National Programme “ACI Microbiologie” Contract No. MIC 03 29.
- Published
- 2008
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