1. CARMA3 Mediates Allergic Lung Inflammation in Response to Alternaria alternata
- Author
-
Benjamin Causton, Benjamin D. Medoff, Jayaraj Rajagopal, Ramnik J. Xavier, Katherine Discipio, Josalyn L. Cho, Ana Pardo-Saganta, Tristan Kooistra, and Jacob Gillis
- Subjects
0301 basic medicine ,Pulmonary and Respiratory Medicine ,medicine.medical_treatment ,Clinical Biochemistry ,Inflammation ,Alternaria alternata ,Alternariosis ,Allergic inflammation ,03 medical and health sciences ,Mice ,0302 clinical medicine ,Immune system ,medicine ,Animals ,Humans ,Receptor ,Molecular Biology ,Cells, Cultured ,biology ,Innate lymphoid cell ,Alternaria ,Cell Biology ,Pneumonia ,respiratory system ,Allergens ,biology.organism_classification ,Asthma ,respiratory tract diseases ,CARD Signaling Adaptor Proteins ,Disease Models, Animal ,030104 developmental biology ,Cytokine ,Immunology ,Respiratory epithelium ,medicine.symptom ,030215 immunology - Abstract
The airway epithelial cell (AEC) response to allergens helps initiate and propagate allergic inflammation in asthma. CARMA3 is a scaffold protein that mediates G protein-coupled receptor-induced NF-κB activation in airway epithelium. In this study, we demonstrate that mice with CARMA3-deficient AECs have reduced airway inflammation, as well as reduced type 2 cytokine levels in response to Alternaria alternata. These mice also have reduced production of IL-33 and IL-25, and reduced numbers of innate lymphoid cells in the lung. We also show that CARMA3-deficient human AECs have decreased production of proasthmatic mediators in response to A. alternata. Finally, we show that CARMA3 interacts with inositol 1,4,5-trisphosphate receptors in AECs, and that inhibition of CARMA3 signaling reduces A. alternata-induced intracellular calcium release. In conclusion, we show that CARMA3 signaling in AECs helps mediate A. alternata-induced allergic airway inflammation, and that CARMA3 is an important signaling molecule for type 2 immune responses in the lung.
- Published
- 2018