15 results on '"Tao, Cong"'
Search Results
2. SCN11A gene deletion causes sensorineural hearing loss by impairing the ribbon synapses and auditory nerves
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Shi Li Zhang, Wei Guo Shi, Fei Ji, Yue Zhang, David Z.Z. He, Wei Sun, Shi Ming Yang, Tao Cong, Xin Song, Wei Wei Guo, and Mian Zu
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Hearing Loss, Sensorineural ,Stereocilia (inner ear) ,Expression ,Biology ,Ribbon synapse ,lcsh:RC321-571 ,Lesion ,Mice ,03 medical and health sciences ,Cellular and Molecular Neuroscience ,0302 clinical medicine ,medicine ,otorhinolaryngologic diseases ,Animals ,Auditory system ,Cochlear Nerve ,NAV1.9 Voltage-Gated Sodium Channel ,lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry ,Cochlea ,030304 developmental biology ,Mice, Knockout ,Mice, Inbred ICR ,0303 health sciences ,Progressive hearing loss ,Hair Cells, Auditory, Inner ,General Neuroscience ,lcsh:QP351-495 ,medicine.disease ,Nav1.9 knockout ,CTBP2 ,Cell biology ,medicine.anatomical_structure ,lcsh:Neurophysiology and neuropsychology ,Synaptopathy ,SGN ,Synapses ,Sensorineural hearing loss ,sense organs ,medicine.symptom ,Gene Deletion ,030217 neurology & neurosurgery ,Research Article ,TTX resistant sodium channels - Abstract
Background The SCN11A gene, encoded Nav1.9 TTX resistant sodium channels, is a main effector in peripheral inflammation related pain in nociceptive neurons. The role of SCN11A gene in the auditory system has not been well characterized. We therefore examined the expression of SCN11A in the murine cochlea, the morphological and physiological features of Nav1.9 knockout (KO) ICR mice. Results Nav1.9 expression was found in the primary afferent endings beneath the inner hair cells (IHCs). The relative quantitative expression of Nav1.9 mRNA in modiolus of wild-type (WT) mice remains unchanged from P0 to P60. The number of presynaptic CtBP2 puncta in Nav1.9 KO mice was significantly lower than WT. In addition, the number of SGNs in Nav1.9 KO mice was also less than WT in the basal turn, but not in the apical and middle turns. There was no lesion in the somas and stereocilia of hair cells in Nav1.9 KO mice. Furthermore, Nav1.9 KO mice showed higher and progressive elevated ABR threshold at 16 kHz, and a significant increase in CAP thresholds. Conclusions These data suggest a role of Nav1.9 in regulating the function of ribbon synapses and the auditory nerves. The impairment induced by Nav1.9 gene deletion mimics the characters of cochlear synaptopathy.
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- 2021
3. Blockage of UCHL1 activity attenuates cardiac remodeling in spontaneously hypertensive rats
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Ting-ting Fu, Hui-Hua Li, Yun-Long Zhang, Xiao Han, Pang-Bo Li, and Tao Cong
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medicine.medical_specialty ,Indoles ,Physiology ,Drug Evaluation, Preclinical ,Inflammation ,Blood Pressure ,Cardiomegaly ,030204 cardiovascular system & hematology ,medicine.disease_cause ,Rats, Inbred WKY ,Muscle hypertrophy ,03 medical and health sciences ,0302 clinical medicine ,Fibrosis ,Internal medicine ,Rats, Inbred SHR ,Oximes ,Internal Medicine ,medicine ,Animals ,030212 general & internal medicine ,STAT3 ,Protein kinase B ,biology ,business.industry ,Myocardium ,PTEN Phosphohydrolase ,medicine.disease ,Oxidative Stress ,Blood pressure ,Endocrinology ,Heart failure ,Hypertension ,biology.protein ,medicine.symptom ,Cardiology and Cardiovascular Medicine ,business ,Ubiquitin Thiolesterase ,Oxidative stress ,Signal Transduction - Abstract
Cardiac remodeling is an important pathological process ultimately leading to heart failure. Ubiquitin carboxy-terminal hydrolase 1 (UCHL1) is a deubiquitinase that plays a critical role in neurodegenerative diseases and cancer. However, its role in cardiac remodeling in spontaneously hypertensive rats remains unclear. Wistar-Kyoto (WKY) rats and spontaneously hypertensive rats (SHRs) were administered the UCHL1 inhibitor LDN-57444 (20 μg/kg/day) from 2 months of age for 4 months. Blood pressure, cardiac hypertrophy, fibrosis, inflammation, and oxidative stress were evaluated by the tail-cuff system, echocardiography, and histological analysis. Gene and protein expression levels were examined by real-time PCR and immunoblotting analysis. At 6 months of age, the expression of UCHL at the mRNA and protein levels was significantly upregulated in SHRs compared with WKYs. Moreover, systolic blood pressure, cardiac performance, left ventricular (LV) hypertrophy, fibrosis, inflammation, and superoxide production were significantly increased in SHRs compared with WKYs, and these effects were markedly attenuated by LDN-57444 after 4 months of administration. These beneficial actions were possibly associated with a reduction in blood pressure and inactivation of multiple signaling pathways, including AKT, ERK1/2, STAT3, calcineurin A, TGF-β/Smad2/3, and NF-κB. In conclusion, the results indicate that UCHL1 is involved in hypertensive cardiac remodeling in SHRs, and targeting UCHL1 activity may be a novel potential therapeutic approach for the treatment of hypertensive heart diseases.
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- 2019
4. A naphthalene derivative as 'turn-on' fluorescent chemosensor for the highly selective and sensitive detection of Al
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Zhong-Xin, Zhu, Dong-Dong, Yu, Zhi-Guo, Liu, Ye-Wei, Huang, A-Yi, Zhou, Zhi-Wei, Chen, Jia, Sun, Xu, Wang, Li-Tai, Jin, and Wei-Tao, Cong
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Spectrometry, Mass, Electrospray Ionization ,Magnetic Resonance Spectroscopy ,Molecular Structure ,Hydrogen-Ion Concentration ,Naphthalenes ,PC12 Cells ,Sensitivity and Specificity ,Molecular Imaging ,Rats ,Spectrometry, Fluorescence ,Limit of Detection ,Spectroscopy, Fourier Transform Infrared ,Animals ,Schiff Bases ,Aluminum ,Fluorescent Dyes - Abstract
A Schiff base compound derived from naphthalene has been synthesized and characterized as an Al
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- 2016
5. [Effects of zinc deficiency on the relevant immune function in rats with sepsis induced by endotoxin/lipopolysaccharide]
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Feng, Li, Tao, Cong, Zhen, Li, and Lin, Zhao
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Lipopolysaccharides ,Interferon-gamma ,Zinc ,Tumor Necrosis Factor-alpha ,Sepsis ,Animals ,Cytokines ,Interleukin-4 ,Interleukin-10 ,Rats - Abstract
To investigate the effects of zinc deficiency on the relevant immune function in rats with LPS-induced sepsis.Sixty rats were divided into low zinc group (LZ), normal zinc pair-fed group (NP), and normal zinc control group (NC) according to the random number table, with 20 rats in each group. The rats in group LZ were fed with low zinc diet, and the rats in group NP were fed with normal zinc diet, with the same intake as that of group LZ by manual control, and the rats in group NC were fed with normal zinc diet freely. After being fed for 7 d, the rats all fasted and were further divide into the below subgroups named LZ-LPS, LZ-normal saline (NS), NP-LPS, NP-NS, NC-LPS, and NC-NS according to the random number table, with 10 rats in each subgroup. Rats in the LPS subgroups were intraperitoneally injected with 1 mg/mL LPS solution with the dosage of 5 mg/kg, rats in the corresponding NS subgroups were intraperitoneally injected with equivalent NS. The rats were sacrificed at post injection hour 6 to collect blood, spleen, and thymus. The serum level of zinc was detected by inductively coupled plasma mass spectrometry, and the serum alkaline phosphatase (ALP) activity was detected by automatic blood biochemical analyzer. The body weight and weight of spleen and thymus of rats were weighed, and the indices of spleen and thymus were calculated. Six routine blood indices were examined by automatic blood cell analyzer. The serum levels of interferon gamma (IFN-γ), TNF-α, IL-4, and IL-10 were determined with ELISA, and the ratio of IFN-γ to IL-4 was calculated. Data were processed with one-way analysis of variance and SNK test.(1) Serum levels of zinc and ALP activity in the LPS subgroups were significantly lower than those in the corresponding NS subgroups (with P values below 0.05). The two former indices in subgroups NP-NS and NC-NS were significantly higher than those in subgroup LZ-NS (with P values below 0.05). The two former indices in subgroups NP-LPS and NC-LPS were significantly higher than those in subgroup LZ-LPS (with P values below 0.05). (2) Body weight, spleen and thymus weight, indices of spleen and thymus in the LPS subgroups were similar with those in the corresponding NS subgroups (with P values above 0.05). The 4 former indices, except for body weight, in subgroups NP-NS and NC-NS were significantly higher than those in subgroup LZ-NS (with P values below 0.05). The 4 former indices, except for body weight, in subgroups NP-LPS and NC-LPS were significantly higher than those in subgroup LZ-LPS (with P values below 0.05). (3) Levels of leucocyte count in subgroups LZ-LPS and NP-LPS were significantly higher than those in the corresponding NS subgroups (with P values below 0.05). Level of leucocyte count in subgroup NC-NS was significantly higher than that in subgroup LZ-NS (P0.05). Level of leucocyte count in subgroup NC-LPS was significantly lower than that in subgroup LZ-LPS (P0.05). Levels of neutrophilic granulocyte count (NGC) and NG in the LPS subgroups were significantly higher than those in the corresponding NS subgroups (with P values below 0.05). The two former indices in subgroup NC-LPS were significantly lower than those in subgroup LZ-LPS (with P values below 0.05). Level of NG in subgroup NC-NS was significantly lower than that in subgroup LZ-NS (P0.05). Levels of lymphocyte count and lymphocyte in subgroups LZ-NS, LZ-LPS, NP-NS, NP-LPS, NC-NS, and NC-LPS were respectively (1.8 ± 0.4) × 10⁹/L, (1.0 ± 0.3)× 10⁹/L, (2.6 ± 0.7) × 10⁹/L, (1.4 ± 0.4) × 10⁹/L, (3.3 ± 0.6) × 10⁹/L, (1.5 ± 0.5) × 10⁹/L, and 0.39 ± 0.10, 0.11 ± 0.03, 0.47 ± 0.12, 0.14 ± 0.04, 0.50 ± 0.09, 0.24 ± 0.07. The two former indices in the LPS subgroups were significantly lower than those in the corresponding NS subgroups (with P values below 0.05). The two former indices in subgroup NC-NS were significantly higher than those in subgroup LZ-NS (with P values below 0.05). The two former indices in subgroups NP-LPS and NC-LPS were significantly higher than those in subgroup LZ-LPS (with P values below 0.05). Level of lymphocyte count in subgroup NP-NS was significantly higher than that in subgroup LZ-NS (P0.05). Levels of platelet count (PC) in subgroups NP-LPS and NC-LPS were significantly lower than those in the corresponding NS subgroups (with P values below 0.05). Levels of PC in subgroups NP-NS and NC-NS were significantly higher than those in subgroup LZ-NS (with P values below 0.05). Level of PC in subgroup NC-LPS was significantly higher than that in subgroup LZ-LPS (P0.05). (4) Serum levels of TNF-α, IL-4, and IL-10 in each subgroup showed no significant differences (with P values above 0.05). Serum levels of IFN-γ and ratios of IFN-γ to IL-4 in subgroups LZ-NS, LZ-LPS, NP-NS, NP-LPS, NC-NS, and NC-LPS were respectively (75 ± 21), (233 ± 40), (80 ± 14), (345 ± 74), (66 ± 7), (821 ± 189) pg/mL, and 3.1 ± 1.0, 6.6 ± 1.7, 3.9 ± 1.7, 20.2 ± 8.3, 3.4 ± 1.5, 45.7 ± 7.6. The two former indices in the LPS subgroups were significantly higher than those in the corresponding NS subgroups (with P values below 0.05). The two former indices in subgroups NP-NS and NC-NS were similar with those in subgroup LZ-NS (with P values above 0.05). The two former indices in subgroups NP-LPS and NC-LPS were significantly higher than those in subgroup LZ-LPS (with P values below 0.05).Zinc deficiency can induce the atrophy of spleen and thymus, and reduction of peripheral blood lymphocyte. In sepsis, zinc deficiency can further decrease the production of IFN-γ, thus making the cytokines of Th1/Th2 shift to Th2 and the immune imbalance worse.
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- 2015
6. GSK-3 Phosphorylates δ-Catenin and Negatively Regulates Its Stability via Ubiquitination/Proteosome-mediated Proteolysis
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Jinhyung No, Hyunkyoung Ki, Ja-Hye Park, Ilhwan Yang, Qun Lu, Inho Kwon, Minsoo Oh, Jung-Kap Choi, Moon-Chang Baek, Kwonseop Kim, Wei-Tao Cong, Hangun Kim, and Sonja K. Bareiss
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Proteasome Endopeptidase Complex ,Delta Catenin ,Proteolysis ,macromolecular substances ,Biology ,Biochemistry ,Rats, Sprague-Dawley ,Glycogen Synthase Kinase 3 ,Mice ,GSK-3 ,medicine ,Animals ,Humans ,Enzyme Inhibitors ,Phosphorylation ,Molecular Biology ,GSK3B ,Neurons ,Glycogen Synthase Kinase 3 beta ,medicine.diagnostic_test ,Ubiquitin ,Kinase ,Protein Synthesis, Post-Translational Modification, and Degradation ,Wild type ,Catenins ,Cell Biology ,Fibroblasts ,Phosphoproteins ,Molecular biology ,Rats ,Cell biology ,Proteasome ,Culture Media, Conditioned ,Catenin ,Cell Adhesion Molecules - Abstract
Delta-catenin was first identified because of its interaction with presenilin-1, and its aberrant expression has been reported in various human tumors and in patients with Cri-du-Chat syndrome, a form of mental retardation. However, the mechanism whereby delta-catenin is regulated in cells has not been fully elucidated. We investigated the possibility that glycogen-synthase kinase-3 (GSK-3) phosphorylates delta-catenin and thus affects its stability. Initially, we found that the level of delta-catenin was greater and the half-life of delta-catenin was longer in GSK-3beta(-/-) fibroblasts than those in GSK-3beta(+/+) fibroblasts. Furthermore, four different approaches designed to specifically inhibit GSK-3 activity, i.e. GSK-3-specific chemical inhibitors, Wnt-3a conditioned media, small interfering RNAs, and GSK-3alpha and -3beta kinase dead constructs, consistently showed that the levels of endogenous delta-catenin in CWR22Rv-1 prostate carcinoma cells and primary cortical neurons were increased by inhibiting GSK-3 activity. In addition, it was found that both GSK-3alpha and -3beta interact with and phosphorylate delta-catenin. The phosphorylation of DeltaC207-delta-catenin (lacking 207 C-terminal residues) and T1078A delta-catenin by GSK-3 was noticeably reduced compared with that of wild type delta-catenin, and the data from liquid chromatography-tandem mass spectrometry analyses suggest that the Thr(1078) residue of delta-catenin is one of the GSK-3 phosphorylation sites. Treatment with MG132 or ALLN, specific inhibitors of proteosome-dependent proteolysis, increased delta-catenin levels and caused an accumulation of ubiquitinated delta-catenin. It was also found that GSK-3 triggers the ubiquitination of delta-catenin. These results suggest that GSK-3 interacts with and phosphorylates delta-catenin and thereby negatively affects its stability by enabling its ubiquitination/proteosome-mediated proteolysis.
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- 2009
7. Proteome response to ochratoxin A-induced apoptotic cell death in mouse hippocampal HT22 cells
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Yeojin Bang, Seung Jun Kwack, Jung-Kap Choi, Somy Yoon, Hyun Jin Choi, Chul Su Yoon, Wei-Tao Cong, Tae Seok Kang, Sang No Lee, and Kwang Youl Lee
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Programmed cell death ,Proteome ,DNA damage ,Tetrazolium Salts ,Apoptosis ,Biology ,Toxicology ,medicine.disease_cause ,Hippocampus ,Mice ,Neuroblastoma ,Cell Line, Tumor ,medicine ,Animals ,Humans ,Electrophoresis, Gel, Two-Dimensional ,Viability assay ,Prohibitin ,Cell Line, Transformed ,Analysis of Variance ,Dose-Response Relationship, Drug ,L-Lactate Dehydrogenase ,General Neuroscience ,Neurodegeneration ,Environmental exposure ,Calcium Channel Blockers ,medicine.disease ,Ochratoxins ,Mitochondria ,Thiazoles ,Gene Expression Regulation ,Biochemistry ,Cell culture ,Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ,Reactive Oxygen Species ,Nucleophosmin ,Oxidative stress - Abstract
Mycotoxins are commonly encountered natural products, and are capable of poisoning animals or humans that inhale mold particles from mycotoxin-contaminated foods. Ochratoxin A (OTA) is produced by Aspergillu ochracus and Penicillium verrucosum, and is often found in cereals and agricultural products. Although previous studies have focused on the potent nephrotoxicity and renal carcinogenicity of OTA, more recent studies suggest that it accumulates in the brain and causes oxidative stress and DNA damage in various brain regions and neuronal populations. In the present study, we undertook to investigate the potential harm caused by environmental exposure to OTA in terms of its effects on neuronal cell viability and proteome profiles. OTA was found to significantly reduce the viabilities of human neuroblastoma SH-SY5Y and mouse hippocampal HT22 cells, as assessed by lactic dehydrogenase release into culture media. Generation of reactive oxygen species was detected in OTA-treated SH-SY5Y and HT22 cells, however, caspase activation and increase in p53 phosphorylation were only detected in HT22 cells, and the expressions of several proteins were found to be significantly altered after treating HT22 cells with OTA. Valosin containing protein, prolyl 4-hydroxylase, Atp5b protein, nucleophosmin 1, eukaryotic translation elongation factor 1 delta isoform, ornithine aminotransferase, prohibitin, and peroxiredoxin 6, which have been suggested to be implicated in the pathogenesis of neurodegenerative disorders, were up-regulated. Our findings suggest that coordinated regulations of molecular networks are involved in the OTA-induced cytotoxicity and that proteome response can be an indicative for neurodegeneration.
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- 2009
8. Anti-fibrotic effects of the Masson pine pollen aqueous extract on hepatic fibrosis rat model
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Tao, Cong, Xue-Yuan, Jin, Lin, Zhao, Long, Ma, Rui-Sheng, Li, Ping, Zhao, and Chang-Jiang, Guo
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Male ,Plants, Medicinal ,Plant Extracts ,Liver Cirrhosis, Experimental ,Pinus ,Antioxidants ,Matrix Metalloproteinases ,Rats, Sprague-Dawley ,Oxidative Stress ,Gene Expression Regulation ,Liver ,Hepatic Stellate Cells ,Animals ,Cytokines ,Pollen ,Original Article ,Collagen ,Chemical and Drug Induced Liver Injury ,Powders ,Carbon Tetrachloride ,Biomarkers ,Cell Proliferation ,Phytotherapy ,Signal Transduction - Abstract
Aim: To observe the antifibrotic effects of Masson Pine Pollen aqueous extract. Methods: Adult Sprague-Dawley rats were randomly divided into control (CG), hepatic fibrosis model (MG), MPPAE low dose (LG), MPPAE high dose (HG), and MPP original powder (MPPOP; OG) groups. Each group was treated with specific protocols and sacrificed 8 weeks later. Multiple indicators such as serum transaminase, HE staining of the liver tissue, and relevant indexes to fibrosis were determined. Results: Severe hyperplasia of fibrous connective tissues was observed in livers of the MG group rats, while aspartate transaminase and alanine transaminase levels and collagen content obviously increased, superoxide dismutase and glutathione peroxidase activities and MMPs expression decreased, malondialdehyde (MDA) and 8-hydroxy-2’-deoxyguanosine concentrations increased, while mRNA expressions of hepatic stellate cell (HSC)-related cytokines such as transforming growth factor-β1 and platelet-derived growth factor, transcription factors such as nuclear factor-κB p65, and signaling protein α-smooth muscle actin were all increased significantly. Conclusions: MPPAE effectively inhibited the fibrotic process in this CCl4-induced hepatic fibrosis rat model. It may be associated with synergic functions of antioxidant activity, inhibitory activity on HSC proliferation, collagen synthesis, and MMPs expression induction.
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- 2015
9. Effects of Different Proteins on the Metabolism of Zn, Cu, Fe, and Mn in Rats
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Shan-fen Bao, Gui-tang Chen, Lin Zhao, and Tao Cong
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Male ,Apparent absorption ,Chemistry ,Endocrinology, Diabetes and Metabolism ,Biochemistry (medical) ,Clinical Biochemistry ,Fe content ,General Medicine ,Urine ,Metabolism ,Biochemistry ,Diet ,Rats ,Trace Elements ,Rats, Sprague-Dawley ,Inorganic Chemistry ,Zinc ,Dietary protein ,Casein ,Animals ,Soybean protein ,Dietary Proteins ,Food science ,Copper - Abstract
Many factors are known to influence trace element metabolism and one of them is dietary protein. The present study examines the effects of casein, soybean protein, and peanut protein on the metabolism of the Zn, Cu, Fe, and Mn in growing rats. The results showed that Zn, Fe, and Mn excretions in the feces of peanut protein-fed rats (PPFRs) were similar to that of casein-fed rats (CPFRs) (p > 0.05), whereas all of the Zn, Cu, Fe, and Mn excretions in the urine of PPFRs were significantly higher than that of CPFRs (p < 0.05), but its apparent absorption rate (AAR) of Cu, Fe and its apparent retention rate (ARR) of Cu were all higher than that of CPFRs (p < 0.05). Hepatic Zn content of soybean protein-fed rats (SPFRs) was higher than that of CPFRs and PPFRs (p < 0.05 respectively) and serum, renal, and femoral Cu contents of SPFRs were significantly lower; however, hepatic Cu, and renal Mn contents were significantly higher than that of CPFRs (p < 0.05, respectively); The hepatic Fe content of SPFRs was significantly higher than that of CPFRs and PPFRs (p < 0.01, respectively). To sum up, compared to casein, soybean protein might be a good dietary source to make up for Zn and Fe deficiency, and also peanut protein to make up for Cu and Fe deficiency.
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- 2006
10. Fluorescent staining of protein in sodium dodecyl sulfate polyacrylamide gels by salicylaldehyde azine
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Mao-Wei, Ni, Wei-Jian, Ye, Wei-Tao, Cong, Guo-Ying, Hong, Zhong-Xin, Zhu, Yuan-Meng, Duan, Xuan, Zhou, and Li-Tai, Jin
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Brain Chemistry ,Molecular Docking Simulation ,Aldehydes ,Mice ,Acrylic Resins ,Animals ,Proteins ,Sodium Dodecyl Sulfate ,Cattle ,Electrophoresis, Polyacrylamide Gel ,Serum Albumin, Bovine ,Fluorescent Dyes - Abstract
As a non-covalent fluorescence probe, in this study, salicylaldehyde azine (SA) was introduced as a sensitive fluorescence-based dye for detecting proteins both in 1-D and 2-D polyacrylamide electrophoresis gels. Down to 0.2 ng of single protein band could be detected within 1 h, which similars to that of glutaraldehyde (GA)-silver stain, but approximately four times higher than that of SYPRO Ruby fluorescent stain. Furthermore, comparative analysis of the MS compatibility of SA stain with SYPRO Ruby stain indicated that SA stain is compatible with the downstream of protein identification by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Additionally, the probable mechanism of the SA stain was investigated by molecular docking. The results demonstrated that the interaction between SA and protein was mainly contributed by hydrogen bonding and hydrophobic forces.
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- 2013
11. Improved staining of phosphoproteins with high sensitivity in polyacrylamide gels using Stains-All
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Wei-Tao, Cong, Wei-Jian, Ye, Mao, Chen, Ting, Zhao, Zhong-Xin, Zhu, Chao, Niu, Dan-Dan, Ruan, Mao-Wei, Ni, Xuan, Zhou, and Li-Tai, Jin
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Staining and Labeling ,Molecular Sequence Data ,Animals ,Caseins ,Electrophoresis, Polyacrylamide Gel ,Amino Acid Sequence ,Carbocyanines ,Coloring Agents ,Phosphoproteins ,Sensitivity and Specificity - Abstract
An improved Stains-All (ISA) staining method for phosphoproteins in SDS-PAGE was described. Down to 0.5-1 ng phosphoproteins (α-casein, β-casein, or phosvitin) can be successfully selectively detected by ISA stain, which is approximately 120-fold higher than that of original Stains-All stain, but is similar to that of commonly used Pro-Q Diamond stain. Furthermore, unlike the original Stains-All protocol that was time consuming and light unstable, ISA stain could be completed within 60 min without resorting to protect the gels from light during the whole staining procedure. According to the results, it is concluded that ISA stain is a rapid, sensitive, specific, and economic staining method for a broad application to the research of phosphoproteins.
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- 2013
12. Previsible silver staining of protein in electrophoresis gels with mass spectrometry compatibility
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Wei-Tao Cong, Xiaokun Li, Sun-Young Hwang, Litai Jin, and Jung-Kap Choi
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Proteomics ,Silver Staining ,Time Factors ,Biophysics ,Sodium thiosulfate ,Mass spectrometry ,Biochemistry ,Sensitivity and Specificity ,Mass Spectrometry ,Silver stain ,chemistry.chemical_compound ,Rosaniline Dyes ,Animals ,Coloring Agents ,Molecular Biology ,Gel electrophoresis ,Detection limit ,Chromatography ,Silver Staining Method ,Analytic Sample Preparation Methods ,Proteins ,Cell Biology ,Staining ,Electrophoresis ,Zinc ,chemistry ,Costs and Cost Analysis ,Linear Models ,Cattle ,Electrophoresis, Polyacrylamide Gel - Abstract
A convenient silver staining method for protein in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gels is described. The method is previsible, sensitive, and mass spectrometry (MS) compatible. Two visible counter ion dyes, ethyl violet (EV) and zincon (ZC), were used in the first staining solution with a detection limit of 2 to 8 ng/band in approximately 1h. The dye-stained gel can be further stained by silver staining, which is based on acidic silver staining employing ZC with sodium thiosulfate as silver ion sensitizers. Especially, ZC has silver ion reducing power by cleavage of the diazo bond of the dye during silver reduction. The second silver staining can be completed in approximately 1h with a detection limit of 0.2 ng/band.
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- 2008
13. [Effects of zinc supplementation on zinc and calcium levels in serum and tissue in burned rats]
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Li-gen, Li, Zhen-rong, Guo, Lin, Zhao, Shan-fen, Bao, Jia-ke, Chai, Tao, Cong, Zhen, Li, Wen-li, Han, Guang-ming, Lu, and Zhi-yong, Sheng
- Subjects
Male ,Rats, Sprague-Dawley ,Disease Models, Animal ,Random Allocation ,Zinc ,Dietary Supplements ,Animals ,Calcium ,Burns ,Rats - Abstract
To explore the effects of zinc supplementation on zinc and calcium levels in serum and tissue in burned rats.Eighty SD rats were randomly divided into C group (control group without scald, n = 8), and N, W, H groups (each consisting of 24 rats), in which the rats were exposed to scalding resulting in partial thickness burns covering 15% of the total body surface area on the back, and then they were fed with diets containing zinc 40 microg/g in N and W groups, and 80 microg/g in H group. A cream containing zinc 761.1 microg/g was applied on the wound in W group at the same time. Eight rats of each group were sacrificed on day 1, 3 and 7 after scald respectively. Venous blood and samples of liver, femur and scald skin were harvested. Zinc and calcium contents in serum and tissues were determined with atomic absorption spectrophotometer.The serum Zn(2+) levels in N, W groups were lower than C group, however, it was obviously higher in H group (up to 16.2 micromol/L) on day 1 after scald. The liver Zn(2+) showed an increasing tendency in all groups, while Ca(2+) level declined in H group, but increased in N, W group. The bone Zn(2+) and Ca(2+) levels showed a progressive declination in all groups from day 1 to 7 after scald. The changes were more obviously in N group than H group (P0.05). The Zn(2+) content of the scalded skin increased obviously in H group on first day after scald and in W group on 7th day after scald. The Ca(2+) contents of scalded skin showed marked increases in all groups, especially in N group, but least in W group.There are obvious changes in Zn(2+) and Ca(2+) contents of serum and tissues after scald injury and zinc supplementation. The effects of zinc supplementation on calcium level in the tissue need to be further studied.
- Published
- 2006
14. High-Glucose Inhibits Human Fibroblast Cell Migration in Wound Healing via Repression of bFGF-Regulating JNK Phosphorylation
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Wei Tao Cong, Wan Hui Cai, Xi Wang, Hong Bo Ye, Hai Shan Tian, Li Tai Jin, Tie Min Wei, Bin Bin Huang, Yuan Hu Xuan, Yu Ting Zhu, Li Sha Chi, Zhong Xin Zhu, and Yuan Meng Duan
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Proteomics ,medicine.medical_specialty ,MAP Kinase Kinase 4 ,lcsh:Medicine ,Apoptosis ,RAC1 ,Global Health ,Biochemistry ,Diabetes Mellitus, Experimental ,Endocrinology ,Cell Movement ,Internal medicine ,Medicine and Health Sciences ,Animals ,Humans ,Medicine ,Public and Occupational Health ,Phosphorylation ,lcsh:Science ,Fibroblast ,Cells, Cultured ,Diabetic Endocrinology ,Skin repair ,Wound Healing ,Multidisciplinary ,business.industry ,Cell growth ,Fibroblast growth factor receptor 1 ,lcsh:R ,Biology and Life Sciences ,Cell migration ,Fibroblasts ,Rats ,Cell biology ,Glucose ,medicine.anatomical_structure ,lcsh:Q ,Fibroblast Growth Factor 2 ,business ,Wound healing ,Annexin A2 ,Research Article - Abstract
One of the major symptoms of diabetes mellitus (DM) is delayed wound healing, which affects large populations of patients worldwide. However, the underlying mechanism behind this illness remains elusive. Skin wound healing requires a series of coordinated processes, including fibroblast cell proliferation and migration. Here, we simulate DM by application of high glucose (HG) in human foreskin primary fibroblast cells to analyze the molecular mechanism of DM effects on wound healing. The results indicate that HG, at a concentration of 30 mM, delay cell migration, but not cell proliferation. bFGF is known to promote cell migration that partially rescues HG effects on cell migration. Molecular and cell biology studies demonstrated that HG enhanced ROS production and repressed JNK phosphorylation, but did not affect Rac1 activity. JNK and Rac1 activation were known to be important for bFGF regulated cell migration. To further confirm DM effects on skin repair, a type 1 diabetic rat model was established, and we observed the efficacy of bFGF on both normal and diabetic rat skin repair. Furthermore, proteomic studies identified an increase of Annexin A2 protein nitration in HG-stressed fibroblasts and the nitration was protected by activation of bFGF signaling. Treatment with FGFR1 and JNK inhibitors delayed cell migration and increased Annexin A2 nitration levels, indicating that Annexin A2 nitration is modulated by bFGF signaling via activation of JNK. Together with these results, our data suggests that the HG-mediated delay of cell migration is linked to the inhibition of bFGF signaling, specifically through JNK suppression.
- Published
- 2014
15. A de novo silencer causes elimination of MITF-M expression and profound hearing loss in pigs
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Liangpeng Ge, Liming Ren, Shiming Yang, Jianan Li, Mingyao Yang, Yiqing Hu, Yiqiang Zhao, Dan Zhu, Fengming Zhang, Lili Ren, Xi Long, Lidong Zhao, Shihua Zhang, Nan Wu, Haijin Yi, Lei Chen, Siqing Chen, Wei Sun, Zuohua Liu, Xiaoxiang Hu, Yue Zhang, Weiwei Guo, Zongyi Guo, Jingyong Wang, Lan Jing, Zhengquan Yu, Yaofeng Zhao, Lei Zhang, Qingyong Meng, Guoqing Tang, Boyuan Sun, Hui Zhao, Xiangang Zou, Tao Wang, Tao Cong, Zhaohui Hou, Mingzhou Li, Ning Yu, Ke Liu, Suoqiang Zhai, Ying Guo, Tinghuan Zhang, Qianzi Tang, Lijuan Zhang, Ning Li, Jiugang Zhao, Ran Zhang, Jinxiu Li, Tiandong Che, Shilin Tian, and Xiaoqing Bai
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0301 basic medicine ,Transcription, Genetic ,Physiology ,Sus scrofa ,Plant Science ,medicine.disease_cause ,Structural Biology ,Protein Isoforms ,Promoter Regions, Genetic ,Genetics ,Regulation of gene expression ,Mutation ,Agricultural and Biological Sciences(all) ,Waardenburg syndrome ,Chromosome Mapping ,cis-regulatory element ,Microphthalmia-associated transcription factor ,Phenotype ,Cochlea ,medicine.symptom ,General Agricultural and Biological Sciences ,Biotechnology ,Research Article ,Gene isoform ,Hearing loss ,De novo silencer ,Biology ,General Biochemistry, Genetics and Molecular Biology ,03 medical and health sciences ,medicine ,Silencer Elements, Transcriptional ,Animals ,Genetic Testing ,Gene ,Ecology, Evolution, Behavior and Systematics ,Pig ,Microphthalmia-Associated Transcription Factor ,Base Sequence ,Biochemistry, Genetics and Molecular Biology(all) ,Cell Biology ,medicine.disease ,Electrophysiological Phenomena ,Disease Models, Animal ,030104 developmental biology ,Gene Expression Regulation ,Developmental Biology ,MITF-M ,Genome-Wide Association Study - Abstract
Background Genesis of novel gene regulatory modules is largely responsible for morphological and functional evolution. De novo generation of novel cis-regulatory elements (CREs) is much rarer than genomic events that alter existing CREs such as transposition, promoter switching or co-option. Only one case of de novo generation has been reported to date, in fish and without involvement of phenotype alteration. Yet, this event likely occurs in other animals and helps drive genetic/phenotypic variation. Results Using a porcine model of spontaneous hearing loss not previously characterized we performed gene mapping and mutation screening to determine the genetic foundation of the phenotype. We identified a mutation in the non-regulatory region of the melanocyte-specific promoter of microphthalmia-associated transcription factor (MITF) gene that generated a novel silencer. The consequent elimination of expression of the MITF-M isoform led to early degeneration of the intermediate cells of the cochlear stria vascularis and profound hearing loss, as well as depigmentation, all of which resemble the typical phenotype of Waardenburg syndrome in humans. The mutation exclusively affected MITF-M and no other isoforms. The essential function of Mitf-m in hearing development was further validated using a knock-out mouse model. Conclusions Elimination of the MITF-M isoform alone is sufficient to cause deafness and depigmentation. To our knowledge, this study provides the first evidence of a de novo CRE in mammals that produces a systemic functional effect. Electronic supplementary material The online version of this article (doi:10.1186/s12915-016-0273-2) contains supplementary material, which is available to authorized users.
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