1. Mineral trioxide aggregate-induced AMPK activation stimulates odontoblastic differentiation of human dental pulp cells
- Author
-
Sam-Young Park, Sunmi Yang, Sun-Woong Bae, Seon-Mi Kim, Ji-Yeon Jung, Won-Jae Kim, and Yoon-Jung Kim
- Subjects
0206 medical engineering ,Odontoblast differentiation ,02 engineering and technology ,03 medical and health sciences ,0302 clinical medicine ,stomatognathic system ,Western blot ,Lysosome ,medicine ,Animals ,Humans ,Aluminum Compounds ,General Dentistry ,PI3K/AKT/mTOR pathway ,Cells, Cultured ,Dental Pulp ,Extracellular Matrix Proteins ,medicine.diagnostic_test ,Odontoblasts ,Kinase ,Chemistry ,Silicates ,Autophagy ,AMPK ,Cell Differentiation ,Oxides ,030206 dentistry ,Calcium Compounds ,Alkaline Phosphatase ,Phosphoproteins ,020601 biomedical engineering ,Molecular biology ,DMP1 ,Rats ,Drug Combinations ,medicine.anatomical_structure - Abstract
AIM To investigate the role of autophagy in MTA-induced odontoblastic differentiation of human dental pulp cells (HDPCs). METHODOLOGY In MTA-treated HDPCs, odontoblastic differentiation was assessed based on expression levels of dentine sialophosphoprotein (DSPP) and dentine matrix protein 1 (DMP1), alkaline phosphatase activity (ALP) activity by ALP staining and the formation of mineralized nodule by Alizarin red S staining. Expression of microtubule-associated protein 1A/1B-light chain3 (LC3), adenosine monophosphate-activated protein kinase (AMPK)/mammalian target of rapamycin (mTOR) signalling molecules and autophagy-related genes was analysed by Western blot analysis and Acridine orange staining was used to detect autophagic lysosome. For in vivo experiments, tooth cavity preparation models on rat molars were established and the expression of proteins-related odontogenesis and autophagy markers was observed by Immunohistochemistry and Western blot analysis. Kruskal-Wallis with Dunn's multiple comparison was used for statistical analysis. RESULTS Mineral trioxide aggregate (MTA) promoted odontoblastic differentiation of HDPCs, accompanied by autophagy induction, including formation of autophagic lysosome and cleavage of LC3 to LC3II (P
- Published
- 2020