1. Role of intestinal brush border peptidases in the simulated digestion of milk proteins
- Author
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Laura Sánchez-Rivera, Francesco Addeo, Isidra Recio, Gianluca Picariello, Pasquale Ferranti, Beatriz Miralles, Gianfranco Mamone, Picariello, G., Miralles, B., Mamone, G., Sanchez Rivera, L., Recio, I., Addeo, Francesco, Ferranti, Pasquale, and European Commission
- Subjects
Proteases ,food.ingredient ,Brush border ,Swine ,Biology ,Brush border membrane hydrolases ,Whey protein isolate ,Degree of hydrolysi ,food ,Degree of hydrolysis ,In vivo ,Skimmed milk ,medicine ,Animals ,Food science ,Brush border membrane hydrolase ,Chromatography, High Pressure Liquid ,Chromatography, Reverse-Phase ,Microvilli ,In vitro digestion model ,Gastrointestinal digestion ,Milk proteins ,Small intestine ,Bioavailability ,medicine.anatomical_structure ,Jejunum ,Biochemistry ,biology.protein ,In vitro digestion models ,Digestion ,Food Science ,Biotechnology ,Peptide Hydrolases - Abstract
[Scope]: This study aimed to assess the impact of the 'often neglected' intestinal brush border membranes (BBMs) hydrolases on dietary peptides, exploring the possibility that the disintegration of proteins progressed in the small intestine up to a >core> of intrinsically stable oligopeptides, persisting independently on the up-stream breakdown. [Methods and results]: Samples of sodium caseinate, skim milk powder, and whey protein isolate were submitted to in vitro simulated gastropancreatic digestion using two different procedures: (i) a simplified model involving the main compartmental specific proteases; (ii) a static digestion method based on a frameset of parameters inferred from in vivo. The gastroduodenal digesta were further hydrolyzed with peptidases from porcine jejunal BBM. The peptidomes arising from the two digestion models, characterized by combined HPLC and MS techniques, differed to some extent. However, only specific protein domains survived digestion, among which are potential bioactive or immunogenic (food allergy) peptides. The degree of hydrolysis (DH) after BBM digestion (70-77%) practically did not differ between the digestion models and significantly increased the DH after duodenal steps. [Conclusion]: Any in vitro digestion model should be supplemented with a jejunal phase to realistically determine the bioaccessibility and bioavailability of dietary peptides., The research was in part supported by the EU Infogest COST (European Cooperation in Science and Technology) Action FA 1005 “Improving health properties of food by sharing our knowledge on the digestive process” by funding a short scientific mission of G. P. in the laboratories at CIAL-CSIC (Madrid, Spain).
- Published
- 2015