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In this industrial revolution era, university-level education emphasises higher-order thinking skills. This research aims to analyse the effect of implementing the PjB-HOTS learning model on cognitive learning, creative thinking skills, analytical thinking skills, and metacognitive skills of the students studying osmoregulation concepts in Animal Physiology courses. The sample consisted of 61 fifth-semester students divided into two classes. An open-ended test was used to measure students' cognitive learning levels, creative thinking skills, analytical thinking skills, creative thinking skills, and metacognitive skills. The research data were analysed using the Analysis of Covariance (ANCOVA). The results of this research show that the learning model had a significant effect on the cognitive learning gains (0.000< [alpha]= 0.05), creative thinking skill (0.001< [alpha]= 0.05), analytical thinking skill (0.000< [alpha]= 0.05), and metacognitive skill (0.000< [alpha]= 0.05). These results indicate that the combined syntax of PjBL and HOTS can foster students' higher-order thinking skills and improve their cognitive learning gains. We recommend the PjB-HOTS learning model be used for other biological concepts at the university-level.

The California Blackworm ("Lumbriculus variegatus") is a freshwater segmented worm species that has been used by biology instructors as a model system for inquiry-based student investigations. The blackworm dorsal blood vessel pulsation rate is easily quantified. Moreover, this species can facilitate the study of neuromuscular functioning via its photosensitive escape behavior which can be quantified as a segmental reflex rate. Both of these variables can be used to examine the physiological response of the "L. variegatus" circulatory and neuromuscular systems to environmental changes. Because knowledge about this species and its optimal environmental conditions is limited, we studied dorsal vessel pulsation and segmental reflexes of "L. variegatus" maintained at differing lighting, temperature and water cleaning frequency conditions. Our data strongly indicate that "L. variegatus" circulatory and motor functions are significantly affected by environmental conditions. We provide evidence-based recommendations for the careful control of environmental conditions that will allow instructors, students and researchers to collect robust data on "L. variegatus" and better utilize this model organism in their investigations.

Fine needle puncture (FNP) is a widespread technique used to collect cellular samples. Its efficiency can be enhanced by the use of ultrasonography to guide the procedure. Ultrasound-guided FNP is therefore an operator-dependent exam. For this reason, it demands the acquisition of psychomotor skill, ability to recognize structures, and dexterity during the needle puncture. This study describes the development of an artisanal simulator made with gelatin to replace the use of live animals during practical classes in veterinary or medical sciences education. The experimental set consisted of three phases in which the student performed different tasks such as recognition of the target structure with ultrasound and injection of ink (phase 1) or aspiration (phase 2) of its content and evaluation of a parenchymatous organ (liver) and puncture of its surface (phase 3). A survey on the acceptance of the model was carried out, and students filled out a questionnaire elaborated with the visual analog scale system. Participants considered the artisanal model a strong method to teach ultrasound-guided FNP. Other attractive advantages of this simulator are the low manufacturing costs (compared with expensive high-technology devices) and the possibility to replace the use of live animals in practical classes.

The Physiology Core Concept of flow down gradients is a major concept in physiology, as pressure gradients are the key driving force for the bulk flow of fluids in biology. However, students struggle to understand that this principle is foundational to the mechanisms governing bulk flow across diverse physiological systems (e.g., blood flow, phloem sap flow). Our objective was to investigate whether bulk flow items that differ in scenario context (i.e., taxa, amount of scientific terminology, living or nonliving system) or in which aspect of the pressure gradient is kept constant (i.e., starting pressure or pressure gradient) influence undergraduate students' reasoning. Item scenario context did not impact the type of reasoning students used. However, students were more likely to use the Physiology Core Concept of "flow down [pressure] gradients" when the pressure gradient was kept constant and less likely to use this concept when the starting pressure was kept constant. We also investigated whether item scenario context or which aspect of the pressure gradient is kept constant impacted how consistent students were in the type of reasoning they used across two bulk flow items on the same homework. Most students were consistent across item scenario contexts (76%) and aspects of the pressure gradient kept constant (70%). Students who reasoned using "flow down gradients" on the first item were the most consistent (86, 89%), whereas students using "pressures indicate (but don't cause) flow" were the least consistent (43, 34%). Students who are less consistent know that pressure is somehow involved or indicates fluid flow but do not have a firm grasp of the concept of a pressure gradient as the driving force for fluid flow. These findings are the first empirical evidence to support the claim that using Physiology Core Concept reasoning supports transfer of knowledge across different physiological systems.

Analogical reasoning is an important type of cognition often used by experts across domains. Little research, however, has investigated how generating analogies can support college students' self-regulated learning (SRL) of biology. This study therefore evaluated a contextualized cognitive learning strategy intervention designed to teach students to generate analogies as a learning strategy to aid learning within a university biology course. Participants (n = 179) were taught how to generate analogies as a learning strategy to learn about plant and animal physiology. We hypothesized the quality of students' generated analogies would increase over time, and their analogical reasoning, knowledge of cognition (KOC; a component of metacognitive awareness), and course performance would be higher after intervention, controlling for associated pre-intervention values. Regression analyses and repeated-measures analysis of variance indicated a positive relationship between generated-analogy quality and analogical reasoning, and increased analogy quality after intervention. No change in reported KOC was observed, and analogy quality did not predict course performance. Findings extend understanding of strategies that can support college students' biology learning. Researchers and practitioners can leverage our approach to teaching analogies in their own research and classrooms to support students' SRL, analogical reasoning, and learning.

This study assessed the impact of an "active learning" strategy employed alone or in combination with traditional lectures on the learning of mammalian physiology by undergraduate students. The study investigated the impact of three teaching strategies, namely (1) traditional lecture, (2) group discussion alone, and (3) combination of lecture and group discussion. For all strategies, students were given homework in a textbook and they completed written assignments before each session. Every student led the discussion of at least one assigned theme during each group session. The students had no access to the textbook or notes during group discussions. Four examinations (3 in-semester and a final) assessed the students' knowledge of fundamental concepts of physiology of specific organ systems. Part of the final examination reassessed knowledge of previously tested topics. The results show that the teaching modality employed to introduce physiology topics influenced students' learning. The average marginal effect of the lecture þ discussion modality (average improvement linked to lecture þ discussion strategy) on students' performance was 6.45% [95% confidence interval (CI[subscript 95]) (4.73, 8.16), P = 1.74 10[superscript -13]], and the average improvement associated with the discussion-only modality was 5.5% [CI[subscript 95] (3.84, 7.16), P = 7.84 10[superscript -11]]. On average, all class ranks performed better on materials covered under active learning settings than under lecture-only conditions. Moreover, students' performance under combined lecture and discussion conditions is predictive of their overall performance in the course. The results support the positive effect of student-centered learning and demonstrate the efficacy of a combination of lectures and group discussions on learning of physiology by nonmajor students.

Technological, Pedagogical, and Content Knowledge (TPACK) is a teaching framework to integrate technology in teaching. Integrating technology into the curriculum becomes an inseparable part of the best teaching practice. This study aims to produce learning tools on blended learning integrated with the PBLRQA strategy in the form of the lesson plan, evaluation sheets, and moodle-based e-learning media that are valid, practical, and effective in Animal Physiology courses. PBLRQA is an innovative learning strategy integrated between Problem-based Learning (PBL) with reading questioning answering (RQA). This study was Research and Development (R & D) using ADDIE development models (analyze, design, development, implementation, and evaluation). The validity of the product was measured using an expert validation sheet, the practicality of the product was measured using a lecturer and student response questionnaire, and the effectiveness of the product was measured using a student learning independence questionnaire. Based on the results of the study it could be concluded that the lesson plan, evaluation sheets, and moodle-based e-learning were valid, practical and effective in increasing the self-regulated learning of students. It proved that the lesson plan, evaluation sheets, and moodle-based e-learning media that integrate PBLRQA strategies are appropriate to use.

One of the overlapping curriculum designs indicates the mismatch of teaching materials with the cognitive level process and student development, impacting the unstructured qualification of mastery of the material. On the other hand, the breadth and depth of teaching materials in the curriculum must be following students' mental development and level of cognitive processes. This study aims to collect the opinions of science teachers in junior high schools and biology teachers in high schools about the learning continuum design on reproducing living things. The method used is a survey. The study population is a hypothetical population with a convenience sample. The sample involved in this study were 111 teachers in Bantul Regency and Yogyakarta City. The data collection method used a questionnaire distributed through four subject teachers' conference (MGMP). The data obtained were analyzed using descriptive analysis. The results showed several sub-aspects of reproduction of living things that cannot be taught in elementary schools. The material for propagating fungi, protists, bacteria, and viruses starts in grade VII with a cognitive level of C1 (remembering), after previously students received material about types of animals and plants in grade VI (elementary school). Furthermore, the material on reproductive anatomy and physiology can only be given to class IX with cognitive level C2 (understanding) and continued in class X and XI with cognitive level C4 (analyzing). Especially for reproductive anatomy and physiology, fungi were introduced to class VII with C1 level cognitive processes (remembering). This teacher opinion generates a learning continuum grid that can help policymakers improve educational curriculum.

Most undergraduate students do not have practical experience working with model organisms despite having theoretical knowledge of their importance. This practical was developed to offer students experience working with three species of nematode, including "Caenorhabditis elegans." In the first task, students prepare slides of three previously fixed nematode samples and are asked to identify their feeding preferences based on information given to them in a short pre-practical presentation. The second and third tasks allow students to deduce the existence of a functional nervous system in "Caenorhabditis elegans" and then to measure the response of two genotypes, N2 and "slo-1," to different concentrations of an ethanol solution. These tasks demonstrate the heterogeneity of different nematodes and enable students to gain experience in widely applicable skills including microscopy, slide preparation, assay design and data presentation. Students also gain experience in specialised skills such as worm picking, which is essential in "Caenorhabditis elegans" research. This practical can be completed as a standalone and would suit any early year biology course.

The analysis of spontaneous tail coiling (STC) in zebrafish embryos is a functional parameter that allows the study of motor development. It has recently gained relevance as a biomarker to assess the neurotoxicity of environmental substances. Its practicability in the laboratory makes it an excellent pedagogical tool for promoting students' inquiry skills. However, the time and cost of materials and facilities limit their usage in undergraduate laboratories. This study presents the design of a computer-based educational module called ZebraSTMe, which is based on a tail coiling assay and aims to improve science process skills in undergraduate students by connecting them to relevant and novel content. We evaluate students' perception of learning, the quality of materials used, and the knowledge gained. Our results show that students perceived an improvement in their statistical analysis, representation, and discussion of experimental data. Additionally, the students evaluated the quality and ease of use of the materials and provided feedback for revision. A thematic analysis of the opinions revealed that the module activities promoted students' reflection on their professional strengths and weaknesses.

Students in an animal physiology course are required to have completed prerequisite cell biology and genetics courses that include discussion of basic properties and functions of the cell membrane. However, while many students remember basic information about membrane structure, they often have difficulty relating that structure to membrane functions, such as vesicular transport, active transport, osmosis, and current flow across the membrane. To better understand what students recall about the cell membrane, students were given an open-ended prompt to draw what they know about the structure and function of the animal cell membrane. This activity was repeated 1-2 weeks after finishing discussion of the cell membrane in class, with an emphasis on the concepts of membrane transport and a related core concept, flow along gradients. Student responses were analyzed using the conceptual framework for the "cell membrane" core concept published by Michael and Modell (Michael J, Modell H. "Adv Physiol Educ" 43: 373-377, 2019). Before covering this content in class, the majority of submissions included a representation of the cell membrane as a phospholipid bilayer, and a high percentage also included membrane proteins or the fluid mosaic model. Similar percentages of students included these concepts in the postcoverage drawing. However, other components of the conceptual framework were included less frequently or not at all before covering the content in class but improved dramatically afterward. This activity provides information about what students recall from prior coursework and which concepts need to be revisited, and it can provide a complementary assessment of student understanding of the core concept of the cell membrane.

In biology education which is an inseparable discipline of medical and veterinary education, it is of great importance that enabling students to make practice in real conditions and gain knowledge and skills related to their professional fields during their education. It is necessary to adapt the new technologies rapidly to education rather than the use of cadavers or laboratory animals, especially when making practice directly related in human and animal anatomy. For this purpose, virtual and augmented reality applications, which have been developed rapidly in recent years, come to the fore as a highly effective technique with the possibility of interacting visually with the objects they give to the user. In this study, the techniques were examined that used in order to increase the learning performance in biology, anatomy, physiology and experimental animals and the process was examined to develop a specific mobile application on Unity3D application. It is envisaged that the difficulties in providing materials for students and ethical debates on the use of experimental animals can be overcome with the dissemination of augmented reality applications and virtual laboratories developed as a result of procedures such as the needs analysis, lesson or course content and scenario writing processes in addition to software development.

The empowerment of metacognitive skills has an important role in increasing the capacity of critical thinking and active control of students' cognitive processes in learning. Metacognitive skills can be enhanced through the implementation of learning strategies involving higher-order thinking. The RQA, ADI, RQA integrated with ADI are constructivistic learning strategies that can accommodate these skills. The researches specifically examining the correlation between metacognitive skills and critical thinking skills at the implementation of RQA, ADI, and RQA integrated with ADI learning strategies are still rarely found. This research is a correlational research, aiming at revealing the correlation between metacognitive skills and critical thinking skills. The data of metacognitive skills and critical thinking skills are collected by using a valid and reliable essay test. The samples of this research are the fourth semester (IV) biology education students programming Animal Physiology course in the 2016/2017 academic year consisting of 109 students. The results of this research show that (1) there was a significant correlation between metacognitive skills and critical thinking skills at the implementation of the four learning strategies, (2) the regression equations analyzed using Anova obtain regression lines (not coincide), and non parallel regression lines (intersected); meaning that there is a difference in the increase rate and magnitude of the critical thinking skills influenced by the four learning strategies. It is expected that the teachers and the lecturers always strive to improve the quality of the learning process through the implementation of active and constructive learning strategies.

Purpose: This article aims to describe and discuss what local weather landscapes mean to children and how weather implies exploring bodily sensations and capabilities. It does so by following the work of a community artist, working as a kindergarten teacher, over 1 year. Design/Approach/Methods: Through a narrative inquiry approach, which also includes studies of archival data and field notes, we analyze how local and personally experienced weather events imply what we call "cultures of exploration" in institutional practices. The epistemologies cross the specter of cultural--historical, pragmatic, and deep ecological philosophy. Findings: Through this study, we exemplify how experiencing weather is intertwined into pedagogical practices like habituating the body to cope with cold and wet weather, learning about danger in a wild natural landscape, and valuing species as a powerful practice. The descriptions exemplify "cultures of exploration" as a pedagogical approach. Originality/Value: In this time of an increasing climate crisis on our planet, the value of our findings is to foreground new insights, awareness, and knowledge relevant to children; to early-childhood education; and to life and societies at large. We can thus develop methods to better care for, protect, and educate children. This article has the potential to show how weather events are intertwined with everyday institutional practices--as well as how children, through exploration, learn to cope with seasonal weather landscapes and local cultural adaptations.

The effectiveness of animal physiology practical book based on guided inquiry model is the essential factor to achievement student understanding a about animal physiology concepts. The purpose of this study was to observe the effectiveness of animal physiology practical book based on guided inquiry model in Padang State University. The variables measured were affective, cognitive, psychomotor, and students' activities. The data of this pre-experimental research were analyzed using averaged, percentage and standardized with Institutional assessment criteria. The results showed that the mean values were: 90.56 for affective competency (Criteria A), 82 for knowledge (Criteria A), and 89.66 for skills (Criteria A). Meanwhile, the percentage of student activities was 90.74% with very active category. It can be concluded that the effectiveness of practical book based on guided inquiry model was very effective to optimized student competences.

Reflective narrative writing on unfolding ethics case vignettes has the potential to promote values like empathy among medical students, which will prove useful in their future. The objective of this study was to explore the scope of guided reflective narratives on unfolding ethics case vignettes integrated into physiology teaching to promote empathy among first-year medical students. First-year medical students (n = 150) were presented with unfolding case vignettes exploring ethical issues, as part of their physiology curriculum, with questions and prompts for a guided reflective narrative. Feedback about the program was obtained through a semistructured questionnaire. The contents of the students' reflective narratives and feedback were analyzed. The narratives revealed that students empathized with the various individuals involved in medical treatment and research, which was also reflected in student feedback (n = 116; 79%) and high Likert scale scores (74%). The program was considered effective by students in broadening their perspective and promoting critical thinking (n = 129; 88%). Through analysis of reflections, a rubric that would enable the evaluation of students' empathy on a scale of increasing ability to look at a situation from another's point of view emerged. This rubric could be used to quantitatively assess the impact of similar programs. Although divided in their individual opinions, the study population was able to relate to all individuals involved in medical practice and research, recognizing the need for fair and ethical practices. The content analysis led to the development of a rubric to grade empathy.

Problem-Based Learning (PBL) is a constructivist learning with the potential to enhance students' science process skill. The implementation of PBL has revealed many advantages, however, a free exploration from a highly complex environment may result in a heavier workload of memory. Accordingly, practicum method can be used to overcome the shortcomings of PBL with the support of authentic assessment. This research aimed to investigate the effect of PBL through practicum supported by authentic assessment, PBL, and conventional learning on science process skills of Dayak and Malay students in Animal Physiology lectures. The research used a quasi-experimental research design with a pretest-posttest nonequivalent control group design with 3 x 2 factorial design. Data collection involved a science process skill test. The data were analyzed descriptively and inferentially with ANCOVA. Significant differences between groups were assessed with LSD test. The result showed that the learning model influenced students' science process skill. The PBL model implemented through a practicum and supported with authentic assessment was the most influential being significantly different to both PBM model and conventional learning. Ethnicity did not significantly affect the students' science process skill. There was no interaction between learning model and ethnicity toward students' science process skill.

Storytelling can stimulate learning by delivering scientific content within a narrative that increases comprehension and engagement. In this article I describe the coevolutionary arms race between toxic newts and predatory garter snakes. This engaging story centers on the use of a deadly neurotoxin called tetrodotoxin (TTX) as an antipredator defense. Some species of newts contain TTX in their tissues, but resistance to TTX has developed through convergent evolution in garter snakes and other species. TTX resistance results from mutated voltage-gated sodium channels. These channels, whether TTX resistant or not, are found in all animals and are vital to the function of nervous and muscle tissues. Through reciprocal selection, coevolution has created phenotypic matching between toxic newts and TTX-resistant garter snakes across their range in the western United States. In other words, as newts became more poisonous, garter snakes became more resistant. These results and the scientific process behind them are discussed in detail. This story can be used by educators to provide a unifying and engaging backdrop as students learn multiple aspects of biology, such as protein structure, genetics, phylogenetics, electrical signaling, evolution, and the process of science.

This article describes an activity designed to hone students' lay communication skills. After carefully analyzing journal articles that highlight specific aspects of vertebrate physiology, students generate "press releases" to summarize experimental results and suggest potential applications of the research. By producing succinct, jargon-free summaries of current research, along with creative connections between the research and potential real-world applications, students develop skills that allow them to communicate more effectively with the general public.

Learning the physiology of the cardiovascular system can be difficult for students, but the use of active teaching methodologies can help and enhance learning. To facilitate the understanding of how the cardiovascular system works, we built a functional model for Veterinary Medicine students attending a cardiovascular physiology lecture and evaluated their self-reported perceptions of how well they learned. We used simple materials to construct the cardiovascular model, such as bottles of transparent polyethylene terephthalate (PET), transparent tubing, rubber balloons, and rubber pears taken from aneroid manometers. Twenty-four veterinary students attended a traditional cardiovascular physiology lecture before being introduced to the functional model. Each student then filled out a structured questionnaire that assessed their learning perception. The data was analyzed by chi-squared (X[superscript 2]) test. We observed that most students considered the use of the model to be a more efficient and satisfactory learning method (p < 0.05). The functional model proved to be a useful tool for learning cardiovascular system physiology. Through the model, the students were able to visualise the way that important structures function, an outcome that is often not possible in classes involving animal vivisections. Moreover, the model facilitates the teaching of the pathophysiology of the cardiovascular system.

As researchers, teachers, and practitioners we often encounter young professionals and lay adults who do not understand basics of mammalian body temperature regulation. Often their single solid piece of knowledge is that some vertebrates (mammals and birds) are warm-blooded and some (fish, amphibians, and reptile) are cold-blooded, which is incorrect. There are many thermal capabilities and regulatory strategies. We provide basics of body temperature regulation, including definitions, its evolution, examples of body temperature variability, unique examples of hibernation and torpor, and we explain how a better understanding benefits individuals in personal and professional lives. We suggest a simple replacement of the warm-blooded paradigm that can be used to inform even young students. As a starting point, students young and old are familiar with species of mammals (e.g., platypus and opossums) that do not maintain as constant or as warm of a body temperature as humans and dogs. Students also know that humans do not maintain a constant body temperature over time (they have a "temperature" when sick) or all part of their body (they have cold hands and feet outside in winter).

This research aims to analyse the progression of 13-14 year-old students' ideas regarding the concept of energy transfer by means of heat in three biological mechanisms of thermal regulation. The outputs of 270 students, grouped in 77 cooperative groups, are analysed by comparing their explanations before and after conducting an experimental laboratory workshop in which they investigate the topic of energy transfer by means of heat. Students' explanations are analysed by identifying them in a "conceptual landscape" This landscape shows the conceptual frontiers that students must overcome to move from initial ideas to more sophisticated ones. Thus, while at the beginning of the workshop many students explain the thermal regulation mechanisms using the concept of the flow of coldness and give the morphological and behavioural mechanism the ability to generate heat, most students end up conceptualising the studied mechanisms in terms of the heat transfer regulating function from the warm living beings to the cold environment. Although the latter conceptualisation corresponds to an idea that is not the most sophisticated way of describing the transfer of energy through heat, it constitutes a first step in students' concept development towards ideas closer to those that are scientifically accepted.

Cetacean watching is an increasingly popular economic activity in Ecuador for tourism operators. Despite government regulations, cetacean watching can have a negative impact on observed animal populations. To enhance good dolphin-watching practices, a course was carried out in Puerto El Morro, Ecuador about sensitisation activities promoting empathy towards and knowledge about bottlenose dolphins, local avifauna and mangrove ecosystems. The course provided tourism operator staff with theoretical and practical knowledge on dolphin physiology and ecology, with a focus on empathy towards the species and on regulations to be respected during the watching tours. The course included interactive workshops, didactic materials, advice, video screening and structured conversations. Two innovative questionnaires were implemented on the boat during 68 dolphin-watching tours to evaluate whether the training course had improved the regulation compliance and on-tour guiding quality of the tour guides and boat drivers. The results showed a statistically significant improvement in dolphin-watching practices in compliance with the "Regulations for the Whale and Dolphin Watching of Ecuador" (p = 0.0002) and in guidance quality (p = 0.0004) after the training course. Boat drivers were identified as influential actors in compliance with regulations and should also be included in the environmental awareness training courses. The study showed that empathy-based sensitisation and knowledge reinforcement positively affect policy compliance and can generate new sustainable approaches for future dolphin-watching activities.

Science essences are product, process and scientific attitude. As a process, science is a used procedure to study the object of study, to discover and to develop science products. It seems that Indonesian education is not focusing on developing students' science process skills. Therefore, the researchers employed a problem-based learning model through an authentic assessment based practicum to improve students' science process skills. A Quasi experimental design with pretest-posttest nonequivalent control group design was applied in this study. Biology educational students of STKIP Persada Khatulistiwa Sintang, West Borneo, Indonesia were taken as the population. The participants of the research were 60 students from two classes of animal physiology, each class consisted of 30 students. Essay test was applied to measure the science process skills. Essay test questions cover both science basic process skills and integrated science process skills. The research result showed that problem based learning through practical and authentic assessment affected the students' science process skills and there were significant differences between the problem-based learning model through authentic assessment based practicum with problem-based learning model.

Most studies of molecular mechanisms of synaptic plasticity have focused on the sequence of changes either at individual synapses or in the cell nucleus. However, studies of long-term facilitation at "Aplysia" sensory neuron--motor neuron synapses in isolated cell culture suggest two additional features of facilitation. First, that there is also regulation of the number of synaptic contacts between two neurons, which may occur at the level of cell pair-specific branch points in the neuronal arbor. Branch points contain many molecules that are involved in protein synthesis-dependent long-term facilitation including neurotrophins and the RNA binding protein CPEB. Second, the regulation involves homeostatic feedback and tends to keep the total number of contacts between two neurons at a fairly constant level both at rest and following facilitation. That raises the question of how facilitation and homeostasis can coexist. A possible answer is suggested by the findings that they both involve spontaneous transmission and postsynaptic Ca[superscript 2]+, which can have bidirectional effects similar to LTP and LTD in hippocampus. In addition, long-term facilitation can involve a change in the set point of homeostasis, which could be encoded by plasticity molecules such as CPEB and/or PKM. A computational model based on these ideas can qualitatively simulate the basic features of both facilitation and homeostasis of the number of contacts.

Purpose: The excised canine larynx provides an advantageous experimental framework in the study of voice physiology. In recent years, signal processing methods have been applied to analyze phonations in excised canine larynx experiments. However, phonations have a highly complex and nonstationary nature corresponding to different proportions of regular and chaotic signal elements. Current nonlinear dynamic methods that are used to assess the degree of irregularity in the voice fail to recognize the distribution of voice type components (VTCs). Method: Based on measures of intrinsic dimension, this article presents a method to analyze the VTC distribution of phonations in excised canine larynx experiments. Thirty-nine phonation samples from 13 excised canine larynges at three different subglottal pressures were analyzed. Results: Phonation produced with subglottal pressures above phonation instability pressure (PIP) and below phonation threshold pressure (PTP) resulted in high proportions of Voice Types 3 and 4, characterized by chaotic and noisy signals. Phonation produced with pressure between PTP and PIP contained mostly Type 1 voice, characterized by a regular and nearly periodic signal. Mean proportions of all VTCs varied significantly in comparisons of phonations produced with Sub-PTP and PTP as well as in comparisons of phonations produced with PTP and PIP. Conclusions: Across all VTCs, the VTC profiles of normal and abnormal phonation differ significantly. Normal phonation is strongly associated with VTC[subscript 1] (Voice Type Component 1), whereas abnormal phonation exhibits increased VTC[subscript 4] (Voice Type Component 4). The study further demonstrates the ability of intrinsic dimension to successfully detect multiple voice types in an acoustic signal and highlights the need for expanded use of intrinsic dimension in human voice.

The purpose of this study is to determine whether viewing narrated video clips improves student learning. The study was conducted with undergraduate, mostly Biology majors, in an Animal Physiology course held in successive semesters. When both classes were given the same face-to-face lectures and identical online resources their performance on an exam with the same multiple choice questions was not statistically different (two-tailed, unpaired t-test). However, when one group was also given unlimited online access to narrated video clips, these students performed statistically better on a second exam with identical multiple choice questions. An attitudinal survey showed that students used the video clips as an introduction to the interactive animations and simulations and as standalone mini-lectures, and they indicated that viewing the clips was the best and most efficient way to learn physiological concepts. While this study used narrated video clips to augment traditional face-to-face instruction, they could be used in a flipped-class, a blended class, and for distance learning.

This article describes the results of a study that investigated the use of the dissection of organs in anatomy and physiology classes in Slovenian lower and upper secondary schools. Based on a sample of 485 questionnaires collected from Slovenian lower and upper secondary school students, we can conclude that dissection of mammalian organs during the courses on Human Anatomy would be a preferred activity for the majority of them. Opinions on such practices are positive, and only a minority of students would prefer to opt out. However, the practice is performed only occasionally in regular classes, or even omitted, and a number of students never participate in it. According to the results, we can suggest the dissection of mammalian organs in combination with alternatives, such as 3D models and virtual laboratories, as a preferred strategy to increase knowledge of anatomy and to raise interest in science. However, students should know that the organs they are dissecting were dedicated to human consumption, or are waste products in these processes. Opt-out options should be provided for those who do not want to participate in such activities.

Certain physiology concepts can be difficult for students to understand, and new strategies need to be implemented to teach these concepts. Cell signaling is a core concept in physiology and is presented to undergraduate students starting with their first-year Principles of Biology course. Flipped teaching (FT) combined with dramatizations were used to teach steroid hormone and protein hormone cell signaling in an Animal Physiology course. Student knowledge level improved, as demonstrated by posttest scores compared to pretest scores. Their confidence level in the material improved after the dramatization activities were completed. In conclusion, the combination of FT with dramatizations enhances student learning and confidence level.

While metabolism is a fundamental process in physiology, the measurement of metabolism by living animals often requires specialized and expensive equipment. In 2016, I developed a novel laboratory exercise for animal physiology students where students were able to observe the effects of temperature and long-term temperature acclimation on the physiology of the Eastern grass shrimp, "Palaemonetes paludosus." In this lab, grass shrimp were housed for 1 week at one of three temperatures, 20°C, 24°C, and 28°C. While grass shrimp encounter temperatures considerably colder than this in the wild, we were limited by the temperatures we could achieve without access to aquarium chillers. The students then moved shrimp into water of different temperatures and measured metabolic rates indirectly using heart rate and oxygen consumption. The initial results showed noticeable shifts in baseline metabolic rate over a relatively short, 2 week long acclimation period. In both heart rate and oxygen consumption, metabolic rates climbed predictably with temperature, consistent with the literature on responses in ectotherms. Also, consistent with the literature, the shrimp that were acclimated to warmer temperatures downregulated their metabolism to compensate, showing the lower metabolic rate at all three temperatures.

As the academic and social-emotional needs of students in schools continue to increase, so too does the presence of dogs in educational spaces. This article aims to present an overview of past and present animal-assisted intervention practices in school settings. This comprehensive literature review examines the current state of research within this field of study. Data from 29 publications were selected according to strict inclusion and exclusion criteria. The results highlight three categories in which the presence of dogs in schools have an impact: social-emotional, cognitive, and physiological. Challenges to program implementation include health risks, cultural context, and negative effects on the animal. Due to the lack of school-based research, more study is needed, especially in order to understand the effect of dogs on the social-emotional learning of students. Finally, the welfare and training of the animals involved should be taken into consideration, and regulations regarding handler and animal training should be enforced.

Purpose: The Comprehensive School Physical Activity Program (CSPAP) model has recently been adopted as a national framework for physical activity (PA) promotion in schools. As a result, there is a need to develop an instrument to help leaders of PA measure policies and practices across the five component areas of a CSPAP. To address this gap, our team systematically developed and assessed the psychometric properties of the CSPAP-Q, an instrument designed to assess school policies and practices related to the five components of CSPAP. Methods: The instrument development process began by reviewing existing school health and PA surveys. The Delphi method was then used to evaluate face and content validity through expert feedback. Thirty-eight physical education teachers completed the CSPAP-Q twice over a 14-day period to further establish validity and to assess test-retest reliability. Results: In total, 117 items were identified for the initial review. Upon completion of expert feedback, 22 items were eliminated and 66 items were revised. After reliability testing, three additional items were eliminated due to low test-retest agreement levels (Kappa <0.40; non-significant X[superscript 2]). Ninety-two items were retained in the final version of the CSPAP-Q and kappa values indicated moderate to substantial agreement among items. Conclusions: After validity and reliability testing, results suggest that the CSPAP-Q is a valid and reliable tool for assessing policies and practices related to CSPAP.

Purpose: This study aimed to evaluate whether supplementation with L-arginine alone or in combination with physical exercise training can modulate rats' lipid and inflammatory profiles after a single intense exercise session. Methods: Male Wistar rats were divided into four different groups: control (C), trained (T), supplemented with L-arginine (C + A) and trained and supplemented (T + A). Animals from supplemented groups (C + A and T + A groups) received 300 mg/kg animal body weight L-arginine diluted in 30 mL of drinking water for 8 weeks. Exercise training protocol (moderate intensity--70% achieved in the maximum effort test) was held 5 days/week for 8 weeks. Results: Exercise training induced a decrease in the amount of plasma, cholesterol and triglyceride totals, and skeletal muscle VEGF and CINC. Supplementation alone showed a benefit by reducing LDL levels. Conclusion: Training combined with supplementation showed a pronounced reduction in skeletal muscle VEGF and CINC amount. L-arginine supplementation, especially when associated with the regular aerobic physical exercise at moderate intensity was able to improve not only plasma lipid profile but also the inflammatory response of skeletal muscle immediately after an exhaustive physical exercise session.

Dopamine plays a critical role in behavioral tasks requiring interval timing (time perception in a seconds-to-minutes range). Although some studies demonstrate the role of dopamine receptors as a controller of the speed of the internal clock, other studies demonstrate their role as a controller of motivation. Both D1 dopamine receptors (D1DRs) and D2 dopamine receptors (D2DRs) within the dorsal striatum may play a role in interval timing because the dorsal striatum contains rich D1DRs and D2DRs. However, relative to D2DRs, the precise role of D1DRs within the dorsal striatum in interval timing is unclear. To address this issue, rats were trained on the peak-interval 20-sec procedure, and D1DR antagonist SCH23390 was infused into the bilateral dorsocentral striatum before behavioral sessions. Our results showed that the D1DR blockade drastically reduced the maximum response rate and increased the time to start responses with no effects on the time to terminate responses. These findings suggest that the D1DRs within the dorsal striatum are required for motivation to respond, but not for modulation of the internal clock speed.

The main purpose of the study was to investigate pre-service teachers' perceptions of the criteria used to classify animals. In this regard, it was also aimed at exploring conceptual structures and reasoning patterns shaping the pre-service teachers' perceptions. The participants of the present study were 324 pre-service teachers attending science education and biology education departments in Turkey. With the aim of gaining insight into pre-service teachers' perceptions of animal classification, qualitative research method was followed in which the participants were interviewed. In addition to qualitative data, quantitative data were also collected by classification forms and in this way it was aimed to bring variety to analysis results and reinforce them. As a result of the study, the conceptual structures shaping the pre-service teachers' perceptions leading to their evaluations are subsumed under the categories of "morphological, anatomic and physiologic characteristics", "behaviour", "habitat" and "systematic". In light of the findings of the current study, it can be argued that some characteristics of animals were given greater priority while classifying them and this leads to development of some erroneous information and alternative conceptions. More holistic evaluation of the characteristics of animals may allow the development of a better perception of animal classification.

There has been an increasing movement towards the introduction of inquiry based learning in undergraduate physiology laboratories. Students can however find this challenging when there is a sudden transition from traditional didactic practicals to full inquiry based activities. One reason for this could be the students' perceptions about the introduction of inquiry based learning. The aim of this preliminary scoping investigation was to judge students' attitudes towards inquiry based learning following a practical containing an element of inquiry. Students were supplied with approximately 2.5cm gastrointestinal ileal loops from guinea-pigs which they connected via a force transducer to recording equipment. Students were also provided with 4 agonists and 4 antagonists for which they had to determine which antagonist paired with which agonist and which order to add the drugs. After the practical students completed a questionnaire and survey. Twenty nine percent of students strongly agreed and 54% agreed that detailed instructions should be given about how to carry out each practical. In answer to the statement I'd value the opportunity to design and carry out my own experiments, 12% strongly agreed, whilst 27% agreed and 48% were neutral. The commonest anxieties expressed by the students were that they would not understand the practical and not getting the right results. These findings suggest that considerable thought needs to be given as to how to best introduce physiology students to inquiry based practicals and that these student cohorts may benefit from some more scoping activities. Some ideas for these scoping activities are presented.

We have studied the intensity of free radical and antioxidant processes in organs (liver, spleen, thymus, lymph nodes of the small intestine, and adrenal glands) and cells (lymphocytes) in the long-term period after combined exposure to a sublethal dose of ?-radiation (6 Gy) and emotional stress. Combined exposure was followed by accumulation of LPO CD and MDA (conjugated dienes and malonic dialdehyde) products in homogenates of the studied organs. To achieve the goal set, experiments were conducted on 40 outbred white mature male rats randomized into groups. The results of the study indicate major changes in lipid peroxidation and antioxidant system under emotional and radiation stress. Ionizing radiation has a dominating role under combined emotional and radiation aftereffect. This resulted in inhibition of antioxidant protection enzymes and development of dual-oxidative stress in experimental animals. Impaired functional relationships of catalytic redox system of glutathione accompanied by inhibiting orientation of changes in activity of glutathione-dependent enzymes, and prolonged tension of the links of antioxidant system can eventually lead to a decrease in antioxidant status of the body, which indicates the need for the development of advanced methods for adaptive correction.

This study aims at performing a sample application of the educational use of Facebook as a social networking site in Animal Physiology classes, and to determine student's' views on the application. The research sample was composed of 29 third year undergraduate students attending the Biology Education Department of Hacettepe University. The Applied part of the research was undertaken during the Spring semester of the 2014-2015 academic year in Animal Physiology classes. A Facebook group called "BIO 314 Animal Physiology" was created for sharing purposes in relation to the course content. The lecturer, as well as the students, shared the group for fourteen weeks on several occasions, and made comments on instances of sharing. In addition, the lecturer assigned the students tasks for the activities and the students were expected to fulfill the tasks. Students' views were obtained through a survey of open-ended questions prepared as the tool of data collection, then the views were put to qualitative analysis. The results showed that the use of Facebook for educational purposes made factors such as sharing, helping each other, increasing communication, reaching the lecturer and visualising the content, easier for students, but there were also students who held negative views on the issue.

Learning and memory involves a large neural network of many brain regions, including the notable hippocampus along with the retrosplenial cortex (RSC) and lateral septum (LS). Previous studies have established that the dorsal hippocampus (dHPC) plays a critical role during the acquisition and retrieval/expression of episodic memories. However, the role of downstream circuitry from the dHPC, including the dHPC-to-RSC and dHPC-to-LS pathways, has come under scrutiny only recently. Here, we used an optogenetic approach with contextual fear conditioning in mice to determine whether the above two pathways are involved in acquisition and expression of contextual fear memory. We found that a selective inhibition of the dHPC neuronal terminals in either the RSC or LS during acquisition impaired subsequent memory performance, suggesting that both the dHPC-to-RSC and dHPC-to-LS pathways play a critical role in memory acquisition. We also selectively inhibited the two dHPC efferent pathways during memory retrieval and found a differential effect on memory performance. These results indicate the intricacies of memory processing and that hippocampal efferents to cortical and subcortical regions may be differentially involved in aspects of physiological and cognitive memory processes.

Despite identical learning experiences, individuals differ in the memory formed of those experiences. Molecular mechanisms that control the neurophysiological bases of long-term memory formation might control how precisely the memory formed reflects the actually perceived experience. Memory formed with sensory specificity determines its utility for selectively cueing subsequent behavior, even in novel situations. Here, a rodent model of auditory learning capitalized on individual differences in learning-induced auditory neuroplasticity to identify and characterize neural substrates for sound-specific (vs. general) memory of the training signal's acoustic frequency. Animals that behaviorally revealed a naturally induced signal-"specific" memory exhibited long-lasting signal-specific neurophysiological plasticity in auditory cortical and subcortical evoked responses. Animals with "general" memories did not exhibit learning-induced changes in these same measures. Manipulating a histone deacetylase during memory consolidation biased animals to have more signal-specific memory. Individual differences validated this brain-behavior relationship in both natural and manipulated memory formation, such that the degree of change in sensory cortical and subcortical neurophysiological responses could be used to predict the behavioral precision of memory.

Purpose: The larynx plays a role in swallowing, respiration, and voice production. All three functions change during ontogeny. We investigated ontogenetic shape changes using a mouse model to inform our understanding of how laryngeal form and function are integrated. We understand the characterization of developmental changes to larynx anatomy as a critical step toward using rodent models to study human vocal communication disorders. Method: Contrast-enhanced micro-computed tomography image stacks were used to generate three-dimensional reconstructions of the CD-1 mouse ("Mus musculus") laryngeal cartilaginous framework. Then, we quantified size and shape in four age groups: pups, weanlings, young, and old adults using a combination of landmark and linear morphometrics. We analyzed postnatal patterns of growth and shape in the laryngeal skeleton, as well as morphological integration among four laryngeal cartilages using geometric morphometric methods. Acoustic analysis of vocal patterns was employed to investigate morphological and functional integration. Results: Four cartilages scaled with negative allometry on body mass. Additionally, thyroid, arytenoid, and epiglottic cartilages, but not the cricoid cartilage, showed shape change associated with developmental age. A test for modularity between the four cartilages suggests greater independence of thyroid cartilage shape, hinting at the importance of embryological origin during postnatal development. Finally, mean fundamental frequency, but not fundamental frequency range, varied predictably with size. Conclusion: In a mouse model, the four main laryngeal cartilages do not develop uniformly throughout the first 12 months of life. High-dimensional shape analysis effectively quantified variation in shape across development and in relation to size, as well as clarifying patterns of covariation in shape among cartilages and possibly the ventral pouch.

This study compares four learning environments: face-to-face learning (F2F), fully e-learning (EL), blended learning (BL), and flipped classroom (FC) with respect to students' learning performance. Moreover, this present research studies changes in perceived flexibility, intrinsic motivation, self-efficacy beliefs of students, and the interaction effects in these student variables on learning performance. Two learning environment design elements: (1) lectures (2) group discussions building on guiding questions, were manipulated to create the four learning environments. Third-year undergraduate students (n = 106), enrolled in the "Animal and Human Physiology" course at CanTho University (Vietnam), were randomly assigned to one of the four learning environments. The results suggest a significant positive differential effect on learning performance when studying in a FC and BL setting. No significant interaction effects could be observed regarding changes in perceived flexibility, intrinsic motivation and self-efficacy. However, significant differences between learning conditions were observed in perceived flexibility. Analysis of focus group data corroborate the finding that students experience more flexibility in time and place when studying in FC, BL and EL environments. In addition, students in a FC environment reflect significantly larger positive changes in their self-efficacy. But, the qualitative data show how positive perceptions about flexibility, motivation and self-efficacy are often cancelled out by negative perceptions.

Translational research means different things to different people. In the biomedical research community, translational research is the process of applying knowledge from basic biology and clinical trials to techniques and tools that address critical medical needs such as new therapies. Translational research then is a "bench to bedside" bridge specifically designed to improve health outcomes (Wetmore & Garner, 2010). In this sense, animal models or cell culture systems may be used to learn about basic underlying genetic and physiologic systems that are exceedingly difficult to study in human subjects (Reeves et al., 2019). This has been a major theme in Down syndrome (DS) research since the mid-1980s when mouse models that approximate the condition of trisomy 21 (Ts21) first became available (Das & Reeves 2011). Translational research has recently taken on a more expansive meaning, as the process of turning observations from the laboratory, the clinic, and the community can all lead to new therapeutic approaches to improve population health outcomes (Rubio et al., 2010). This model has received increased attention in the last decade as it is clear that improving developmental outcomes for people with DS requires a community effort on the part of all stakeholders (Capone, 2010).

While results from many past studies have implicated the bed nucleus of the stria terminalis (BNST) in mediating the expression of sustained negative affect, recent studies have highlighted a more complex role for BNST that includes aspects of fear learning in addition to defensive responding. As BNST is thought to encode ambiguous or unpredictable threat, it seems plausible that it may be involved in encoding early cued fear learning, especially immediately following a first tone-shock pairing when the conditioned stimulus-unconditioned stimulus (CS-US) contingency is not fully apparent. To investigate this, we conducted in vivo electrophysiological recording studies to examine neural dynamics of BNST units during cued fear acquisition and recall. We identified two functionally distinct subpopulations of BNST neurons that encode the intertrial interval (ITI) and may contribute to within- and across-session fear learning. "Ramping" cell activity during cued fear acquisition parallels the increase in freezing expression as mice learn the CS-US contingency, while "Phasic" cells encode postshock (US[subscript post]) periods (30 sec following encounter with footshock) only during early trials. Importantly, the magnitude of Phasic unit responsivity to the first US[subscript post] period predicted not only freezing expression in response to the subsequent CS during acquisition, but also CS freezing evoked 24 h later during CS retrieval. These findings suggest for the first time that BNST activity may serve as an instructive signal during cued fear learning.

Growth hormone (GH) deficiency is associated with cognitive decline which occur both in normal aging and in endocrine disorders. Several brain areas express receptors for GH although their functional role is unclear. To determine how GH affects the capacity for learning and memory by specific actions in one of the key areas, the hippocampus, we injected recombinant adeno-associated viruses (rAAVs) in male rats to express green fluorescent protein (GFP) combined with either GH, antagonizing GH (aGH), or no hormone, in the dorsal CA1. We found that aGH disrupted memory in the Morris water maze task, and that aGH treated animals needed more training to relearn a novel goal location. In a one-trial spontaneous location recognition test, the GH treated rats had better memory performance for object locations than the two other groups. Histological examinations revealed that GH increased the dendritic spine density on apical dendrites of CA1, while aGH reduced the spine density. GH increased the relative amount of immature spines, while aGH decreased the same amount. Our results imply that GH is a neuromodulator with strong influence over hippocampal plasticity and relational memory by mechanisms involving modulation of dendritic spines. The findings are significant to the increasing aging population and GH deficiency patients.

Objective: Consuming less water (systemic dehydration) has long been thought to dehydrate the vocal folds. An "in vivo," repeated measures study tested the assumption that systemic dehydration causes vocal fold dehydration. Proton density (PD)-weighted magnetic resonance imaging (MRI) of rat vocal folds was employed to investigate (a) whether varying magnitudes of systemic dehydration would dehydrate the vocal folds and (b) whether systemic rehydration would rehydrate the vocal folds. Method: Male (n = 25) and female (n = 14) Sprague Dawley rats were imaged with 7T MRI, and normalized PD-weighted signal intensities were obtained at predehydration, following dehydration, and following rehydration. Animals were dehydrated to 1 of 3 levels by water withholding to induce body weight loss: mild (< 6% body weight loss), moderate (6%-10% body weight loss), and marked (> 10% body weight loss). Results: There was a significant decrease in vocal fold signal intensities after moderate and marked dehydration (p < 0.0167). Rehydration increased the normalized signal intensity to predehydration levels for only the moderate group (p < 0.0167). Normalized signal intensity did not significantly change after mild dehydration or when the mildly dehydrated animals were rehydrated. Additionally, there were no significant differences in PD-weighted MRI normalized signal intensity between male and female rats (p > 0.05). Conclusion: This study provides evidence supporting clinical voice recommendations for rehydration by increasing water intake after an acute, moderate systemic dehydration event. However, acute systemic dehydration of mild levels did not dehydrate the vocal folds as observed by PD-weighted MRI. Future programmatic research will focus on chronic, recurring systemic dehydration.

Over the years, much criticism against animal use for physiology teaching has been made. Hence, replacement by suitable alternatives has increased in several pedagogical approaches. This study examined students' perceptions of animal versus virtual (video/computer) laboratory classes in physiological sciences associated with the effectiveness of the problem-based learning (PBL) hybrid curriculum. Three cohorts of medical students from the University of Ribeirão Preto, who participated in animal or virtual physiology classes or both, were asked to fill out a 5-point Likert questionnaire about knowledge acquisition/motivation, importance to PBL learning goals, skills acquired, need for animal use, academic formation, learning impairment, and alternative methods. We also assessed their grades in the final exam. A total of 350 students were included, in which 108 participated only in virtual classes, 120 only in practical animal laboratory classes, and 122 in both approaches. The majority agreed that the two methods improved their knowledge acquisition/motivation and helped to reinforce tutorial goals and to acquire skills. However, the cohort who experienced both approaches favored animal laboratory. Students believe animal use is needed and did not impair their learning. Conversely, their opinion about academic formation without animal laboratory classes was divided, as was whether this approach inspired them to seek alternative methods. Despite the different perceptions, there was no difference among the groups' final grades (7.3 ± 1 vs. 7.2 ± 1 vs. 7.2 ± 2 for virtual or practical animal laboratory classes or both, respectively). Therefore, virtual activities are not as effective as animal use in the opinions of the students, but they are successful strategies in physiology learning that can be used in practical classes in a hybrid PBL curriculum.

Rats can be used as a model organism to teach physiological concepts in a laboratory setting. This article describes a two-part laboratory that introduces students to hypothesis testing, experimental design, the appropriate use of controls and surgical techniques. Students perform both a castration and sham-control surgery on male rats and test the effects of reduced testosterone due to castration on the weight and histology of seminal vesicles. After performing the surgeries and collecting the data, students learn histological techniques, as well as empirical data collection, analysis and interpretation. Demonstrating the effects of testosterone through castration surgery bridges concepts learned in a traditional physiology class setting with data gleaned from research in a laboratory. Overall, the male castration surgery provides students with hands-on skills and an understanding of the work done by scientific researchers and health care professionals.

A classroom exercise is described in which college students take part in creating and supporting an evolutionary hypothesis that explains effort grunting. The exercise holds their interest throughout and readies them to understand hypotheses of animal and plant evolution. It informs them about the dependence of cultural evolution upon biological evolution, and it connects widely to curricula.

Spatial memory, the aspect of memory involving encoding and retrieval of information regarding one's environment and spatial orientation, is a complex biological function incorporating multiple neuronal networks. Hippocampus-dependent spatial memory is not innate and emerges during development in both humans and rodents. In children, nonhippocampal dependent egocentric (self-to-object) memory develops before hippocampal-dependent allocentric (object-to-object) memory. The onset of allocentric spatial memory abilities in children around 22 mo of age occurs at an age-equivalent time in rodents when spatially tuned grid and place cells arise from patterned activity propagating through the entorhinal-hippocampal circuit. Neuronal activity, often driven by specific sensory signals, is critical for the normal maturation of brain circuits This patterned activity fine-tunes synaptic connectivity of the network and drives the emergence of specific firing necessary for spatial memory. Whereas normal activity patterns are required for circuit maturation, aberrant neuronal activity during development can have major adverse consequences, disrupting the development of spatial memory. Seizures during infancy, involving massive bursts of synchronized network activity, result in impaired spatial memory when animals are tested as adolescents or adults. This impaired spatial memory is accompanied by alterations in spatial and temporal coding of place cells. The molecular mechanisms by which early-life seizures lead to disruptions at the cellular and network levels are now becoming better understood, and provide a target for intervention, potentially leading to improved cognitive outcome in individuals experiencing early-life seizures.