1. CD4 T-cell depletion prevents Lassa fever associated hearing loss in the mouse model
- Author
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Junki Maruyama, Rachel A. Reyna, Megumi Kishimoto-Urata, Shinji Urata, John T. Manning, Nantian Harsell, Rebecca Cook, Cheng Huang, Janko Nikolich-Zugich, Tomoko Makishima, and Slobodan Paessler
- Subjects
CD4-Positive T-Lymphocytes ,Disease Models, Animal ,Mice ,Lassa Fever ,Virology ,Immunology ,Genetics ,Animals ,Parasitology ,Hearing Loss ,Lassa virus ,Molecular Biology ,Microbiology - Abstract
Lassa virus (LASV) is the causative agent of Lassa fever (LF), which presents as a lethal hemorrhagic disease in severe cases. LASV-induced hearing loss in survivors is a huge socioeconomic burden, however, the mechanism(s) leading to hearing loss is unknown. In this study, we evaluate in a mouse LF model the auditory function using auditory brainstem response (ABR) and distortion product otoacoustic emissions (DPOAE) to determine the mechanisms underlying LASV-induced hearing loss. In the process, we pioneered measures of ABR and DPOAE tests in rodents in biosafety level 4 (BSL-4) facilities. Our T cell depletion studies demonstrated that CD4 T-cells play an important role in LASV-induced hearing loss, while CD8 T-cells are critical for the pathogenicity in the acute phase of LASV infection. Results presented in this study may help to develop future countermeasures against acute disease and LASV-induced hearing loss.
- Published
- 2022
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