1. Phylogenetic Analysis of the Dengue Virus Strains Causing the 2019 Dengue Fever Outbreak in Hainan, China
- Author
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Feifei Yin, Xiuji Cui, Chuanning Tang, Liyuan Zhang, Lina Niu, Wenqi Wang, Gaoyu Wang, Gang Lu, Meifang Xiao, Haoxiang Su, Kunliang Wu, Xiaoyuan Hu, Yi Huang, Yongguo Du, Fan Yang, Ruoyan Peng, Yun Zhang, Jiang Du, and Qiang Wang
- Subjects
0301 basic medicine ,China ,Genotype ,030106 microbiology ,Immunology ,Dengue fever patients ,Dengue virus ,medicine.disease_cause ,Mosquito larvae ,Virus ,Disease Outbreaks ,Dengue fever ,Dengue ,03 medical and health sciences ,Aedes ,Virology ,medicine ,Animals ,Humans ,Pathogen ,Phylogeny ,biology ,Transmission (medicine) ,Aedes spp. mosquitos ,DENV-1 ,Outbreak ,Dengue Virus ,medicine.disease ,biology.organism_classification ,030104 developmental biology ,Hainan Province ,Molecular Medicine ,Research Article - Abstract
Dengue virus is an arthropod-borne pathogen that is transmitted to humans primarily by Aedes spp. mosquitos, causing the acute infectious disease, dengue fever (DF). Until 2019, no dengue outbreak had been reported in Hainan Province for over 20 years. However, in early September of 2019, an increasing number of infected cases appeared and the DF outbreak lasted for over one month in Haikou City, Hainan Province. In our study, we collected 97 plasma samples from DF patients at three hospitals, as well as 1585 mosquito larvae samples from puddles in different areas of Haikou. There were 49 (50.5%) plasma samples found to be strongly positive and 9 (9.3%) plasma samples were weakly positive against the NS1 antigen. We discovered DENV both in the patient’s plasma samples and mosquito larvae samples, and isolated the virus from C6/36 cells inoculated with the acute phase serum of patients. Phylogenetic analysis revealed that the new strains were the most closely related to the epidemic strain in the southern regions of China, belonging to lineage IV, genotype I, DENV-1. Compared to the seven closest strains from neighboring countries and provinces, a total of 18 amino acid mutations occurred in the coding sequences (CDS) of the new isolated strain, DENV1 HMU-HKU-2. Our data shows that dengue virus is re-emerged in Hainan, and pose new threats for public health. Thus regular molecular epidemiological surveillance is necessary for control and prevention of DENV transmission. Electronic supplementary material The online version of this article (10.1007/s12250-020-00335-x) contains supplementary material, which is available to authorized users.
- Published
- 2021