1. Regulation of GluA1 Phosphorylation by D-amphetamine and Methylphenidate in the Cerebellum
- Author
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Christian Lüscher, Alban de Kerchove d'Exaerde, Elsa Isingrini, Emma Puighermanal, Laura Cutando, Vincent Pascoli, Patricia Bonnavion, Laia Castell, Federica Bertaso, Micaela Galante, Pauline Tarot, Bruno Giros, Glenn Dallérac, Emmanuel Valjent, Institut de Génomique Fonctionnelle (IGF), Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS), Universitat Autònoma de Barcelona (UAB), ULB Neuroscience Institute [Brussels] (UNI), Université libre de Bruxelles (ULB), Centre Neurosciences intégratives et Cognition (INCC - UMR 8002), Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP), McGill University = Université McGill [Montréal, Canada], Institut des Neurosciences Paris-Saclay (NeuroPSI), Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), University of Geneva [Switzerland], University of Barcelona, Autonomous University of Barcelona, and Douglas Hospital Research Center, Department of Psychiatry, Faculty of Medicine
- Subjects
Male ,medicine.medical_specialty ,Génétique moléculaire ,Dextroamphetamine ,Monoaminergic system ,[SDV]Life Sciences [q-bio] ,Medicine (miscellaneous) ,AMPA receptor ,03 medical and health sciences ,Norepinephrine ,Mice ,Cerebellar Cortex ,0302 clinical medicine ,Dopamine receptor D1 ,Dopamine ,Internal medicine ,Cerebellum ,medicine ,Bergmann Glia Cells ,Animals ,Phosphorylation ,Prescription stimulants ,Amphetamine ,Pharmacology ,Neurophysiologie ,Methylphenidate ,Chemistry ,Receptors, Dopamine D1 ,Phosphotransferases ,Biologie moléculaire ,monoaminergic system ,3. Good health ,030227 psychiatry ,ddc:616.8 ,Psychiatry and Mental health ,Endocrinology ,nervous system ,prescription stimulants ,Cerebellar cortex ,Central Nervous System Stimulants ,[SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC] ,cerebellar cortex ,030217 neurology & neurosurgery ,Bergmann glial cells ,GluA1 ,medicine.drug - Abstract
Prescription stimulants, such as d-amphetamine or methylphenidate are used to treat suffering from attention-deficit hyperactivity disorder (ADHD). They potently release dopamine (DA) and norepinephrine (NE) and cause phosphorylation of the α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor subunit GluA1 in the striatum. Whether other brain regions are also affected remains elusive. Here, we demonstrate that d-amphetamine and methylphenidate increase phosphorylation at Ser845 (pS845-GluA1) in the membrane fraction of mouse cerebellum homogenate. We identify Bergmann glial cells as the source of pS845-GluA1 and demonstrate a requirement for intact NE release. Consequently, d-amphetamine-induced pS845-GluA1 was prevented by β1-adenoreceptor antagonist, whereas the blockade of DA D1 receptor had no effect. Together, these results indicate that NE regulates GluA1 phosphorylation in Bergmann glial cells in response to prescription stimulants., SCOPUS: ar.j, info:eu-repo/semantics/published
- Published
- 2021