1. Characterization of a flow-sorted human chromosome 10 cosmid library by FISH
- Author
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L.L. Deaven, J. Mao, J.L. Longmire, Y. Benchekroun, N.S.-F. Ma, C. Zheng, and D.T. Moir
- Subjects
Molecular Sequence Data ,Biology ,Hybrid Cells ,Cell Line ,Gene mapping ,Cricetinae ,Genetics ,Animals ,Humans ,Molecular Biology ,Genetics (clinical) ,In Situ Hybridization, Fluorescence ,Gene Library ,Chromosomes, Human, Pair 10 ,Chromosome ,Chromosome Mapping ,Cosmids ,Flow Cytometry ,Molecular biology ,Chromosome 17 (human) ,Genetic marker ,Cosmid ,Molecular probe ,Low copy number ,DNA Probes ,Chromosome 22 - Abstract
Fluoresence in situ hybridization (FISH) was used to localize cosmids to regions of human chromosome 10. A total of 301 cosmids were selected randomly from a flow-sorted human chromosome 10 cosmid library constructed from human × hamster cell line 762–8A and arrayed in microtiter storage dishes. Over 70% (211/301) of the cosmids mapped to unique regions of chromosome 10. About 7% (22/301) produced multiple hybridization signals indicative of chimeric clones or sequences repeated at low copy number. Three cosmids (3/301, or 1 %) hybridized to the centromeric regions of chromosome 10 and one or more other human chromosomes. About 19% (59/301) consisted mostly or entirely of hamster DNA inserts, and about 2% (6/301) appeared to be nonrecombinants.
- Published
- 1996