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Physiological and biomedical experiments were performed. We estimated the parameters of tree main levels of physiological systems as a response of an organism to stress. Mechanisms of physiological defense of an organism against stress should be considered systemically. The response of physiological systems of animal organism was modeled by the activation of latent functional reserves by various exposures to stress (abdominal surgery, cranial electrotherapy stimulation, cranial electrotherapy stimulation with simultaneous administration of droperidol, aminazine, vetranquil, and carbacholine).

We have studied the effects of chlorpromazine, acepromazine, droperidol, and transcranial electroanesthesia upon evacuation function of the stomach in piglets and the effects of leu-enkephalin and glycyl-proline upon secretory activity of the stomach in dogs and rats during surgical stress to optimize anesthetic dosage. All pharmaceutical and nonpharmaceutical methods of anesthesia used in the experiments were implemented by actiovating stress-limiting systems in post-operational period. Leu-enkephalin, apart from stimulating the stress-limiting factors of neurohumoral systems of the organism, shifted the vector ratio of aggressive and protective factors of gastric mucosa toward strengthening of the latter. The opposite trends of changes in the secretory activity of the stomach of rats in response to surgical stress can be leveled using glycyl-proline as a component of anesthesia.

Experiments were performed on pathogenicity of LPV oncornavirus and Mason-Pfizer-like virus for monkeys Macaca mulatta. The mixture of culture fluid and cell homogenate ultrafiltrates of virus-producing T-9 cell line was used as a source of LPV virus. The same materials derived from HEp-2 continuous cell line served as a source of Mason-Pfizer-like virus. As a control monkeys were inoculated with ultrafiltrates from human diploid cells. The clinical and pathomorphological analysis of experimental results leads to the conclusion, that only LPV virus induced different forms of haemacytoblastosis in monkeys that confirms our previous observations.

Some properties of 4 diplod cell strains derived from the lung and skin-muscle tissue of human embryos were studied. Only one strain (L-63) was shown to have a stable karyotype throughout its life span and induced no tumor formation in the cheek pouch of hamsters. Species-specific and group antigens were revealed. In the cell strains of the lung and skin-muscle derivation differences in the distribution of lactate dehydrogenase isoenzymes were observed and found characteristic of the original tissue cells.

Two strains of diploid cells were prepared from the lung (BEL-2) and skin-muscle (SMBE-3) tissues of a bovine embryo. According to the results of morphological and karyological studies, these cultures consisted of fibroblast-like cells retaining diploid chromosome number (60) in the process of long-term cultivation. These cell populations have a limited life span (75-80 passages) and 3 growth phases. The species appurtenance of BEL-2 and SMBE-3 cells was confirmed. The cells were not contaminated with mycoplasma or extraneous viruses, had no tumorigenic activity. The BEL-2 cell culture is most susceptible to measles virus, SMBE-3 culture to vaccinia virus.

Glucose 6-phosphate dehydrogenase (E. C. 1.1.1.49) was found to be inactivated by 30% for 25 min the incubation at 37 degrees C in extracts of human diploid cells and mouse liver, and the stability of the enzyme was found to reduce in aging of human diploid cells and mouse. The stability of dehydrogenase of mice treated with "geroprotector", 2-ethyl-6-methyl-3-hydroxypyridine, exceeds the control level.

Different strains of diploid cells were studied experimentally in Syrian hamsters by heterotransplantation into cheek pouch tissue for detection of their tumorigenic activity. Cortisone and antilymphocyte serum were used as immunodepressors. The results showed that the use of antilymphocyte serum increased considerably the sensitivity of the method of determination of cell culture tumorigenic activity.