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DP-3Ø5423-1 (305423) is a genetically modified soybean that was produced by biolistic insertion of the gm-fad2-1 gene fragment and gm-hra genes into the germline of soybean seeds. Expression of gm-fad2-1 results in greater concentrations of oleic acid (18:1) by suppressing expression of the endogenous FAD2-1 gene, which encodes an n-6 fatty acid desaturase enzyme that catalyzes desaturation of 18:1 to linoleic acid (18:2). The GM-HRA protein expressed by the gm-hra gene is a modified version of the soybean acetolactate synthase enzyme that is used as a selectable marker during transformation. A 42-d feeding trial was conducted with broiler chickens to compare the nutritional performance of 305423 soybeans with nontransgenic soybeans. Diets were prepared using processed fractions (meal, hulls, and oil) from 305423 soybean plants. For comparison, additional diets were produced with soybean fractions obtained from a nontransgenic near-isoline (control) and nontransgenic commercial Pioneer brand varieties (93B86, 93B15, and 93M40). Diets were fed to Ross x Cobb broilers (n = 120/group, 50% male and 50% female) in 3 phases. Starter, grower, and finisher diets contained 26.5, 23, and 21.5% soybean meal, respectively. Soybean hulls and oil were added at 1.0 and 0.5%, respectively, across all diets in each phase. No statistically significant differences were observed in growth performance (BW, mortality, feed efficiency), organ yield (liver and kidney), or carcass yield (breast, thigh, leg, wing, and abdominal fat) variables between broilers consuming diets prepared with isolated fractions from 305423 or near-isoline control soybean. Additionally, all performance and carcass variables from control and 305423 soybean treatment groups fell within tolerance intervals constructed for each response variable using data from broilers fed diets prepared with reference soybean fractions. Based on the results from this study, it was concluded that 305423 soybeans were nutritionally equivalent to non-transgenic control soybeans with a comparable genetic background.

Maize line 1507, containing event DAS-Ø15Ø7-1 (1507), is a genetically modified (GM) maize plant that expresses the cry1F gene from Bacillus thuringiensis (Bt) sbsp. aizawai and the phosphinothricin-N-acetyltransferase (pat) gene from Streptomyces viridochromogenes throughout the plant including in the grain expression of the Cry1F protein confers in planta resistance to the European corn borer (ECB; Ostrinia nubilalis Hübner: Crambidae) and other lepidopteran pests. Expression of the PAT protein confers tolerance to the herbicidal active ingredient glufosinate-ammonium. The current study evaluated the nutritional performance of rats fed diets containing 1507 maize grain in a subchronic rodent feeding study. The grains in this study, 1507, its near-isogenic control (33P66), and a non-GM commercial hybrid (33J56) contained similar amounts of proximates, amino acids, minerals, anti-nutrients, and secondary metabolites. The subchronic feeding study compared standard toxicology response variables in rats fed diets containing 1507 maize grain with those in rats fed diets containing non-GM maize grains. All diets were prepared according to the specifications of PMI Nutrition International, LLC Certified Rodent LabDiet 5002 (PMI) 5002). Diets were fed ad libitum to Sprague-Dawley rats for approximately 90 days. In-life response variables included indicators of dietary performance and weekly evaluations for clinical signs of toxicity. No toxicologically significant differences were observed in the nutritional performance variables, clinical and neurobehavioral signs, ophthalmology, clinical pathology (hematology, clinical chemistry, coagulation, and urinalysis), organ weights, and gross and microscopic pathology between any pair of treatment groups. These results demonstrate that 1507 maize grain is as safe and as nutritious as non-GM maize grain.

A genetically modified maize (Zea mays L.) line that contains the Optimum GAT trait (event DP-O9814O-6; 98140) was produced by integration of the gat4621 and zm-hra genes. The expressed GAT4621 and ZM-HRA proteins confer tolerance to the herbicidal active ingredient glyphosate and acetolactate synthase-inhibiting herbicides, respectively. The objective of this study was to compare the nutritional performance of 98140 maize grain to nontransgenic maize grain in a 42-d feeding trial in broiler chickens. Diets were prepared using grain from untreated 98140 plants and from plants treated with an in-field application of herbicides (98140 + Spray). For comparison, additional diets were produced with maize grain obtained from the nontransgenic near-isogenic control (control) and nontransgenic commercial reference Pioneer brand hybrids 33J56, 33P66, and 33R77. Diets were fed to Ross x Cobb broilers (n = 120/group, 50% male and 50% female) in 3 phases: starter, grower, and finisher containing 58.5, 64, and 71.5% maize grain, respectively. No statistically significant differences were observed in mortality, growth performance variables, or carcass and organ yields between broilers consuming diets produced with maize grains from unsprayed or sprayed 98140 and those consuming diets produced with near-isogenic control maize grain. Additionally, all performance and carcass variables from control, 98140, and 98140 + Spray test maize treatment groups were within tolerance intervals constructed using data from reference maize groups. Based on these results, it was concluded that 98140 maize grain (unsprayed or sprayed with a herbicide mixture) was nutritionally equivalent to nontransgenic control maize with comparable genetic background.