1. Glükózamin-szulfát hatékonyságának vizsgálata mononátrium-jodoacetáttal indukált arthrosis modellen patkányban.
- Author
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HRISTIFOR, GÁLITY, ZOLTÁN, AIGNER, PIROSKA, SZABÓ-RÉVÉSZ, GYÖNGYI, HORVÁTH, GELLÉRT, SOHÁR, and KÁLMÁN, TÓTH
- Subjects
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GLUCOSAMINE , *OSTEOARTHRITIS , *DIFFERENTIAL scanning calorimetry , *SULFATES , *ENTHALPY , *ANIMAL disease models , *DRUG therapy , *LABORATORY rats , *THERAPEUTICS - Abstract
Osteoarthritis is the most prevalent joint disease. Glucosamine sulphate has been widely used in humans to treat osteoarthritis for more than two decades, although there are not enough trials to confirm that glucosamine sulphate has therapeutic effect. Differential scanning calorimetry has been widely used for determining physicochemical transformations that occur during thermal degradation. The purpose of this study was to elucidate complex deviations that develop from the normal matrix composition during osteoarthritis and osteoarthritis with glucosamine sulphate treatment contributing to disease progression in monosodium iodoacetate osteoarthritis rat model. Osteoarthritis was induced by the intraarticular injection of monosodium iodoacetate. Glucosamine sulphate solution dissolved in water was administered to rats, two weeks after the monosodium iodoacetate injection. The control group received the same volume of distilled water. For sample preparation the rats were anesthetized and a 2 mm disc was removed from the unhealthy and healthy cartilage surfaces. The thermal properties of samples were determined by differential scanning calorimetry method. With the rise of temperature an endothermic reaction was observed in all cases. The enthalpy change of the process initiated by the temperature change showed marked difference (p<0.0001) between the normal and pathological groups. Interestingly the rats injected with monosodium iodoacetate and pretreated with oral glucosamine sulphate showed significantly higher increase in the value of the enthalpy change than the non-treated but osteoarthritis induced cartilage samples. Characterization of the altered metabolism in cartilage that promotes disease development should lead to future treatment options that can prevent structural damage. [ABSTRACT FROM AUTHOR]
- Published
- 2013