1. Bothrops leucurus venom induces acute hypotension in rats by means of its phospholipase A
- Author
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Thiago N, Menezes, Gustavo B, Naumann, Pollyana, Peixoto, Wender N, Rouver, Helena L, Gomes, Fabiana V, Campos, Marcia H, Borges, Roger L, Dos Santos, Nazaré S, Bissoli, Eladio F, Sanchez, and Suely G, Figueiredo
- Subjects
Phospholipases A2 ,Crotalid Venoms ,Animals ,Bothrops ,Hypotension ,Rats ,Snake Venoms - Abstract
Cardiovascular effects induced by snake venoms, in spite of having a crucial role in the outcome of the envenomation, have been less studied than other toxic activities displayed by these venoms. In this study we evaluated acute cardiovascular responses to Bothrops leucurus venom - Bl-V - both in vivo, in anesthetized rats, and in vitro, in isolated rat mesenteric resistance arteries. Bl-V (10-100 μg protein/kg) caused dose-dependent hypotension, followed by gradual recovery (2-20 min) to basal levels, and induced dose-dependent (1-20 μg/mL) vasodilation in pre-contracted arteries, what was more pronounced when the endothelium remained intact. These effects were partially counteracted by pre-treatment with indomethacin (cyclooxygenase inhibitor). Prior incubation of Bl-V with commercial pentavalent Bothrops antivenom also attenuated the cardiovascular effects induced by the venom, in spite of it not being among the venoms used for the development of the bothropic antivenom. Through an approach based on two chromatographic steps and mass spectrometry (MALDI-ToF and MALDI-ISD), a component with acute cardiovascular effects was isolated and identified as the basic phospholipase blD-PLA
- Published
- 2019