1. Dynamic Activity of miR-125b and miR-93 during Murine Neural Stem Cell Differentiation In Vitro and in the Subventricular Zone Neurogenic Niche
- Author
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Luigi Naldini, Bernhard Gentner, Daniela Corno, Angela Gritti, Frank Speleman, Pieter Mestdagh, Tiziano Di Tomaso, and Annalisa Lattanzi
- Subjects
Male ,SITU HYBRIDIZATION ,Mouse ,Cellular differentiation ,lcsh:Medicine ,Bioinformatics ,ADULT NEUROGENESIS ,Mice ,Neural Stem Cells ,Lateral Ventricles ,Molecular Cell Biology ,BRAIN ,Stem Cell Niche ,lcsh:Science ,Neurons ,Multidisciplinary ,Stem Cells ,Neurogenesis ,Statistics ,PROLIFERATION ,Cell Differentiation ,Animal Models ,MIRNA EXPRESSION ,Neural stem cell ,Cell biology ,medicine.anatomical_structure ,Female ,Viral Vectors ,Cellular Types ,Research Article ,Cell type ,NEURONAL DIFFERENTIATION ,Subventricular zone ,Biology ,Biostatistics ,In Vitro Techniques ,Microbiology ,Vector Biology ,Model Organisms ,Developmental Neuroscience ,microRNA ,medicine ,Animals ,Cell Lineage ,Progenitor cell ,Gliogenesis ,LENTIVIRAL VECTORS ,MICRORNA EXPRESSION ,Gene Expression Profiling ,lcsh:R ,Biology and Life Sciences ,CLUSTER ,MicroRNAs ,nervous system ,RNA ,lcsh:Q ,Mathematics ,Developmental Biology ,Neuroscience - Abstract
Several microRNAs (miRNAs) that are either specifically enriched or highly expressed in neurons and glia have been described, but the identification of miRNAs modulating neural stem cell (NSC) biology remains elusive. In this study, we exploited high throughput miRNA expression profiling to identify candidate miRNAs enriched in NSC/early progenitors derived from the murine subventricular zone (SVZ). Then, we used lentiviral miRNA sensor vectors (LV.miRT) to monitor the activity of shortlisted miRNAs with cellular and temporal resolution during NSC differentiation, taking advantage of in vitro and in vivo models that recapitulate physiological neurogenesis and gliogenesis and using known neuronal- and glial-specific miRNAs as reference. The LV.miRT platform allowed us monitoring endogenous miRNA activity in low represented cell populations within a bulk culture or within the complexity of CNS tissue, with high sensitivity and specificity. In this way we validated and extended previous results on the neuronal-specific miR-124 and the astroglial-specific miR-23a. Importantly, we describe for the first time a cell type- and differentiation stage-specific modulation of miR-93 and miR-125b in SVZ-derived NSC cultures and in the SVZ neurogenic niche in vivo, suggesting key roles of these miRNAs in regulating NSC function.
- Published
- 2013