1. Acute emotional stress and high fat/high fructose diet modulate brain oxidative damage through NrF2 and uric acid in rats
- Author
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Frédéric Canini, Karine Couturier, Nathalie Marissal-Arvy, Cécile Batandier, Anne-Marie Roussel, T. Poyot, Isabelle Hininger-Favier, Laboratory of Fundamental and Applied Bioenergetics = Laboratoire de bioénergétique fondamentale et appliquée (LBFA), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Grenoble Alpes (UGA), Institut de Recherche Biomédicale des Armées (IRBA), Nutrition et Neurobiologie intégrée (NutriNeuro), Université de Bordeaux (UB)-Institut Polytechnique de Bordeaux-Ecole nationale supérieure de chimie, biologie et physique-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), ANR-07-PNRA-0003,CERVIRMIT,Cerveau, résistance à l'insuline et mitochondrie :Rôle neuroprotecteur des polyphénols de la cannelle dans un modèle animal de syndrome métabolique(2007), Université Bordeaux Segalen - Bordeaux 2-Université Sciences et Technologies - Bordeaux 1-Institut Polytechnique de Bordeaux-Ecole nationale supérieure de chimie, biologie et physique-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), and ANR-07-PNRA-0030,TEMPANTIOX,Des procédés innovants pour proposer des produits transformés à base de fruits aux qualités organoleptiques et nutritionelles optimisées.(2007)
- Subjects
Male ,MESH: Oxidation-Reduction ,0301 basic medicine ,Redox signaling ,Antioxidant ,Dietary Sugars ,[SDV]Life Sciences [q-bio] ,Endocrinology, Diabetes and Metabolism ,medicine.medical_treatment ,Psychological Distress ,medicine.disease_cause ,Antioxidants ,MESH: Uric Acid ,chemistry.chemical_compound ,0302 clinical medicine ,Endocrinology ,MESH: Animals ,Acute stress ,chemistry.chemical_classification ,MESH: Oxidative Stress ,Nutrition and Dietetics ,MESH: Dietary Sugars ,MESH: NF-E2-Related Factor 2 ,Brain ,MESH: Reactive Oxygen Species ,MESH: Gene Expression Regulation ,Mitochondria ,Oxidation-Reduction ,medicine.medical_specialty ,MESH: Rats ,NF-E2-Related Factor 2 ,MESH: Mitochondria ,030209 endocrinology & metabolism ,Fructose ,Oxidative phosphorylation ,Diet, High-Fat ,MESH: Psychological Distress ,MESH: Brain ,03 medical and health sciences ,Insulin resistance ,Internal medicine ,High-fat/high-fructose diet ,medicine ,TBARS ,Animals ,Rats, Wistar ,Reactive oxygen species ,030109 nutrition & dietetics ,MESH: Antioxidants ,MESH: Rats, Wistar ,Glutathione ,medicine.disease ,MESH: Male ,Rats ,Uric Acid ,MESH: Diet, High-Fat ,Gene Expression Regulation ,chemistry ,Diet, Western ,Oxidative stress ,Uric acid ,Reactive Oxygen Species ,MESH: Fructose ,MESH: Diet, Western - Abstract
International audience; Studies focusing on the interaction of dietary and acute emotional stress on oxidative stress in cortex frontal and in brain mitochondria are scarce. Dietary-induced insulin resistance, as observed in Western diets, has been associated with increased oxidative stress causing mitochondrial dysfunction. We hypothesized that acute emotional stress could be an aggravating factor by impacting redox status in cortex and brain mitochondria. Thus, the aim of the present study was to evaluate the combination of an insulin resistance inducing high-fat/high-fructose (HF/HFr) diet and acute emotional stress on brain oxidative stress in rats. We measured several oxidative stress parameters (carbonyls, FRAP, TBARS assays, GSH, GSSG, oxidized DNA, mRNA expression of redox proteins (Nrf2), and uric acid). The HF/HFr diet resulted in increased oxidative stress both in the brain mitochondria and in the frontal cortex and decreased expression of the Nrf2 gene. The emotional stress induced an oxidative response in plasma and in brain mitochondria of the control group. In the HF/HFr group it triggered an increase expression of the redox transcription factor Nrf2 and its downstream antioxidant genes. This suggests an improvement of the redox stress tolerance in response to an enhanced production of reactive oxygen species. Accordingly, a blunted oxidative effect on several markers was observed in plasma and brain of HF/HFr-stressed group. This was confirmed in a parallel study using lipopolysaccharide as a stress model. Beside the Nrf2 increase, the stress induced a stronger UA release in HF/HFr which could take a part in the redox stress.
- Published
- 2020