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1. Smooth muscle cells-derived CXCL10 prevents endothelial healing through PI3Kγ-dependent T cells response

Author

Nicole Malet, Hafid Ait-Oufella, Stéphanie Gayral, Paul Ohayon, Icia Santos-Zas, Romain Solinhac, Damien Ramel, Muriel Laffargue, Didier Carrié, Lynda Zeboudj, Jean-François Arnal, Thibault Lhermusier, Mehdi Benamar, Adrien Lupieri, Abdel Saoudi, Natalia F. Smirnova, Emilio Hirsch, Institut des Maladies Métaboliques et Cardiovasculaires (I2MC), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre de Physiopathologie Toulouse Purpan ex IFR 30 et IFR 150 (CPTP), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS), Complejo Hospitalario Universitario de Santiago de Compostela, Paris-Centre de Recherche Cardiovasculaire (PARCC - UMR-S U970), Hôpital Européen Georges Pompidou [APHP] (HEGP), Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Department of Genetics, Biology and Biochemistry, Université de Turin, Service de cardiologie [Toulouse], Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-CHU Toulouse [Toulouse]-Hôpital de Rangueil, CHU Toulouse [Toulouse], Hormones, facteurs de croissance et physiopathologie vasculaire, Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-IFR 31 Louis Bugnard (IFR 31), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Toulouse [Toulouse]-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Toulouse [Toulouse]-Institut National de la Santé et de la Recherche Médicale (INSERM), Departement /u563 : Lipoproteines et Mediateurs Lipidiques, Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Toulouse III - Paul Sabatier (UT3), Centre de Physiopathologie Toulouse Purpan (CPTP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Complejo Hospitalario Universitario de Santiago de Compostela [Saint-Jacques-de-Compostelle, Espagne] (CHUS), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Hôpital de Rangueil, CHU Toulouse [Toulouse]-CHU Toulouse [Toulouse], IFR 31 Louis Bugnard (IFR 31), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Toulouse [Toulouse]-Université Toulouse III - Paul Sabatier (UT3), and Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3)

Subjects
0301 basic medicine, CD4-Positive T-Lymphocytes, Chemokine, Physiology, medicine.medical_treatment, [SDV]Life Sciences [q-bio], [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], 030204 cardiovascular system & hematology, Phosphoinositide 3-kinase, Muscle, Smooth, Vascular, 0302 clinical medicine, Re-Epithelialization, Class Ib Phosphatidylinositol 3-Kinase, ComputingMilieux_MISCELLANEOUS, Cells, Cultured, Mice, Knockout, education.field_of_study, biology, Chemistry, 3. Good health, Cell biology, medicine.anatomical_structure, Cytokine, Smooth muscle cells, Cardiology and Cardiovascular Medicine, Signal Transduction, Endothelium, Population, Myocytes, Smooth Muscle, Cardiovascular events, 03 medical and health sciences, Interferon-gamma, Immune system, [SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system, In vivo, Physiology (medical), Paracrine Communication, medicine, Human Umbilical Vein Endothelial Cells, CXCL10, Animals, Humans, Immune response, education, Cell Proliferation, Wound Healing, Endothelial Cells, Chemokine CXCL10, Mice, Inbred C57BL, Disease Models, Animal, 030104 developmental biology, biology.protein, Endothelial healing, Carotid Artery Injuries, Ex vivo
Abstract

International audience; AimsDefects in efficient endothelial healing have been associated with complication of atherosclerosis such as post-angioplasty neoatherosclerosis and plaque erosion leading to thrombus formation. However, current preventive strategies do not consider re-endothelialization in their design. Here, we investigate mechanisms linking immune processes and defect in re-endothelialization. We especially evaluate if targeting phosphoinositide 3-kinase γ immune processes could restore endothelial healing and identify immune mediators responsible for these defects.Methods and resultsUsing in vivo model of endovascular injury, we showed that both ubiquitous genetic inactivation of PI3Kγ and hematopoietic cell-specific PI3Kγ deletion improved re-endothelialization and that CD4+ T-cell population drives this effect. Accordingly, absence of PI3Kγ activity correlates with a decrease in local IFNγ secretion and its downstream interferon-inducible chemokine CXCL10. CXCL10 neutralization promoted re-endothelialization in vivo as the same level than those observed in absence of PI3Kγ suggesting a role of CXCL10 in re-endothelialization defect. Using a new established ex vivo model of carotid re-endothelialization, we showed that blocking CXCL10 restore the IFNγ-induced inhibition of endothelial healing and identify smooth muscle cells as the source of CXCL10 secretion in response to Th1 cytokine.ConclusionAltogether, these findings expose an unforeseen cellular cross-talk within the arterial wall whereby a PI3Kγ-dependent T-cell response leads to CXCL10 production by smooth muscle cells which in turn inhibits endothelial healing. Therefore, both PI3Kγ and the IFNγ/CXCL10 axis provide novel strategies to promote endothelial healing.

Published
2018
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2. EVER proteins, key elements of the natural anti-human papillomavirus barrier, are regulated upon T-cell activation

Author

Loïc Dupré, Béatrice Pignolet, Maciej Lazarczyk, Roland S. Liblau, Cécile Dalard, Slawomir Majewski, Myriam Hayder, Michel Favre, Françoise Vuillier, Centre de Physiopathologie Toulouse Purpan (CPTP), Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Medical University of Warsaw - Poland, Génétique, Papillomavirus et Cancer Humain, Institut Pasteur [Paris] (IP), This work was supported by Institut national de la santé et de la recherché médicale (INSERM), Agence de Recherche contre le Cancer (ARC), Agence Nationale de la Recherche (ANR-MIE 2009), and Région Midi-Pyrénées. The funders had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript., The authors wish to thank Daniel Gonzalez-Dunia and Abdel Saoudi for their critical comments on the manuscript, Valerie Duplan and Fatima Ezzahra L’Faqihi-Olive for help with the FACS analyses, and Maryline Calise, Patrick Aregui and Aline Tridon (Zootechnic Unit, Purpan) for mouse care., Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), and Institut Pasteur [Paris]

Subjects
Human papillomavirus infection, Skin Neoplasms, T-Lymphocytes, MESH: Base Sequence, Adaptive Immunity, Lymphocyte Activation, Polymerase Chain Reaction, Mice, 030207 dermatology & venereal diseases, 0302 clinical medicine, MESH: Papillomaviridae, Genetics of the Immune System, Homeostasis, MESH: Animals, Lymphocytes, Immune Response, Papillomaviridae, Mice, Inbred BALB C, 0303 health sciences, Multidisciplinary, T Cells, Lymphoblast, Cell biology, Host-Pathogen Interaction, Zinc, medicine.anatomical_structure, [SDV.IMM]Life Sciences [q-bio]/Immunology, Medicine, Female, MESH: Membrane Proteins, Research Article, MESH: DNA Primers, Skin Infections, Cell type, Immune Cells, Mechanisms of Resistance and Susceptibility, T cell, Science, Immunology, MESH: Mice, Inbred BALB C, Cytotoxic T cells, Malignant Skin Neoplasms, Dermatology, Biology, Microbiology, Cell Line, 03 medical and health sciences, Zinc transporters, Virology, medicine, Animals, Humans, MESH: Lymphocyte Activation, MESH: Mice, Immunity to Infections, DNA Primers, 030304 developmental biology, MESH: Humans, Base Sequence, T-cell receptor, Immunity, Membrane Proteins, MESH: Polymerase Chain Reaction, Epidermodysplasia verruciformis, medicine.disease, MESH: Cell Line, MESH: T-Lymphocytes, Membrane protein, Lesions, Clinical Immunology, Skin Carcinoma, MESH: Female, CD8
Abstract

International audience; Human papillomaviruses (HPV) cause a variety of mucosal and skin lesions ranging from benign proliferations to invasive carcinomas. The clinical manifestations of infection are determined by host-related factors that define the natural anti-HPV barrier. Key elements of this barrier are the EVER1 and EVER2 proteins, as deficiency in either one of the EVER proteins leads to Epidermodysplasia Verruciformis (EV), a genodermatosis associated with HPV-induced skin carcinoma. Although EVERs have been shown to regulate zinc homeostasis in keratinocytes, their expression and function in other cell types that may participate to the anti-HPV barrier remain to be investigated. In this work, we demonstrate that EVER genes are expressed in different tissues, and most notably in lymphocytes. Interestingly, in contrast to the skin, where EVER2 transcripts are hardly detectable, EVER genes are both abundantly expressed in murine and human T cells. Activation of CD4+ and CD8+ T cells via the TCR triggers a rapid and profound decrease in EVER expression, accompanied by an accumulation of free Zn(2+) ions. Thus, EVER proteins may be involved in the regulation of cellular zinc homeostasis in lymphocytes. Consistent with this hypothesis, we show that the concentration of Zn(2+) ions is elevated in lymphoblastoid cells or primary T cells from EVER2-deficient patients. Interestingly, we also show that Zn(2+) excess blocks T-cell activation and proliferation. Therefore, EVER proteins appear as key components of the activation-dependent regulation of Zn(2+) concentration in T cells. However, the impact of EVER-deficiency in T cells on EV pathogenesis remains to be elucidated.

Published
2012
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