1. Consequences of supplying methyl donors during pregnancy on the methylome of the offspring from lactating and non-lactating dairy cattle
- Author
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Anna Arís, Isabel Guasch, Alex Bach, Producció Animal, and Producció de Remugants
- Subjects
B Vitamins ,0301 basic medicine ,Physiology ,lcsh:Medicine ,Ice calving ,Biochemistry ,Endocrinology ,Pregnancy ,Reproductive Physiology ,Lactation ,Medicine and Health Sciences ,lcsh:Science ,Mammals ,DNA methylation ,Multidisciplinary ,Organic Compounds ,Eukaryota ,Vitamins ,Ruminants ,04 agricultural and veterinary sciences ,Chromatin ,Nucleic acids ,Dairying ,Chemistry ,medicine.anatomical_structure ,Physiological Parameters ,Physical Sciences ,Vertebrates ,Gestation ,Female ,Epigenetics ,DNA modification ,Chromatin modification ,Research Article ,Chromosome biology ,Cell biology ,Offspring ,Biology ,Placebo ,Cobalamins ,Promoter Regions ,03 medical and health sciences ,Folic Acid ,Bovines ,Genetics ,medicine ,Animals ,Gene Regulation ,Kinase activity ,Biology and life sciences ,Endocrine Physiology ,lcsh:R ,Organic Chemistry ,Body Weight ,Chemical Compounds ,Organisms ,0402 animal and dairy science ,DNA ,medicine.disease ,040201 dairy & animal science ,030104 developmental biology ,Amniotes ,lcsh:Q ,Cattle ,Gene expression - Abstract
The aim of this study was to evaluate the potential effects of methyl donor supplementation of pregnant animals in the presence or absence of a concomitant lactation on the methylome of the offspring. Twenty Holstein cows, 10 nulliparous (non-lactating while pregnant) and 10 multiparous (lactating while pregnant) were blocked by parity and randomly assigned to an i.m. weekly injections of a placebo (CTRL) or a solution containing methyl donors (MET). After calving, 5 calves randomly selected from each treatment (two born to non-lactating and three to lactating dams) were blood-sampled to determine their full methylome. There were more than 2,000 CpG differentially methylated between calves born to CTRL and those born to MET, and also between calves born to lactating and non-lactating dams. Most of the differences affected genes involved in immune function, cell growth regulation and differentiation, kinase activity, and ion channeling. We conclude that the coexistence of pregnancy and lactation affects the methylome of the offspring, and that supplementation of methyl donors early in gestation has also consequences on the methylome. info:eu-repo/semantics/publishedVersion
- Published
- 2017