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The experiment investigated the effect of dietary orange pulp (OP) with or without enzymes supplementation on the performance parameters, chemical composition, serum biochemistry, and some liver gene expression in tilapia for twelve weeks. The studied fish (n = 300) were allotted into six groups according to OP level (0%, 10%, and 20%) with or without enzymes supplementation (+/-). The performance results indicated that the inclusion level of OP by 20% negatively affected FCR and decreased final body weight. The dietary enzymes did not alleviate the unfavorable effect of OP on the final body weight. Higher dietary replacement levels of OP reduced serum lipid profile, serum proteins, and carcass fat, but increased carcass protein and ash compared to the control. The dietary enzymes produced higher serum lipids and proteins as compared to non-supplemented groups. Fish-fed the control diet supplemented with enzymes (G2) expressed higher RNA levels of GHR and IGF1 genes compared to the other groups. Excess dietary OP lowered the RNA level of FAS and LPL genes but improved simultaneously when supplemented with enzymes. Overall, the higher dietary OP (20%) with or without enzymes supplementation, did not benefit the tilapia performance, metabolic, and genetic indices, and the chemical composition of the carcass. But, the lower level of OP (10%) supplemented with enzymes could be used in tilapia diets without adverse effects on performance, health, and carcass composition.

[EN] Utilization of local by-products in pig nutrition can reduce the environmental impact of feeds and contribute to the sustainable development of the livestock sector. Orange pulp (OP) is the most abundant citrus by-product worldwide, but its seasonal production and perishable nature requires storage and drying procedures that might affect its nutritive value. Conservation process by fuel drying is expensive and can impair feed sustainability. Instead, in the Mediterranean countries, OP is sun-dried in the open-air. This procedure often implies a previous silage (during storage) which occurs naturally, because OP has a high level of sugars available for fermentation. Orange pulp is also rich in soluble fiber, which is highly fermentable at the pig's caecum and may reduce gas emissions from slurry. In this study, the nutritive value of conventional fuel-dehydrated (DOP) or ensiled-sun dried (ESDOP) was determined for pig diets. Sugars fermentation during ensiling increases fiber level in ESDOP and decreases energy digestibility compared to DOP, but both OP have an appreciable digestible energy content for pigs, around 87 and 94% that of barley, respectively. In addition, they do not differ in the amount of slurry excreted and contribute to reduce potential derived ammonia and methane emissions. The inclusion of orange pulp (OP) in pig diets may promote the circular economy, but drying procedures might influence its nutritional value and environmental impact. The purpose of this study was to determine the energy value and nutrient digestibility of dehydrated (DOP) and ensiled sun dried (ESDOP) orange pulp. The potential ammonia (NH3) and methane (CH4) emissions derived from slurry were also measured. Digestible energies of 14.2 and 13.2 MJ/kg DM for DOP and ESDOP, respectively, were estimated by difference after a 500 g/kg substitution of a basal diet with OPs. A high fiber digestion efficiency was observed for both OPs. Pigs fed the basal diet showed a higher intake and a greater excretion of urine N than pigs fed with OP, but fecal N excretion did not differ among diets. A higher benzoic and hippuric acid content in urine was observed in OP than in basal diet. Altogether, these findings explained a lower pH in slurry of OP diets and a reduction of potential NH3 emissions. The biochemical CH4 potential also decreased, especially with ESDOP. Overall, both OP are relevant sources of energy for pig diets. Their inclusion in feeds generate favorable changes of slurry characteristics that reduce potential NH3 and CH4 emissions., This work was supported by the Spanish Ministry of Science, Innovation and Universities (AGL2014-56653 and RTI2018-095246).

We analysed how replacing cereal concentrates with dehydrated orange pulp (DOP) in the diet of mother goats affects the meat quality of suckling kids. Three experimental diets for mother goats were designed. The DOP-0 diet contained commercial concentrates and alfalfa hay. In the DOP-40 and DOP-80 diets, 40% and 80% (respectively) of the cereal in the concentrate was replaced with pellets of DOP (the alfalfa hay component was unchanged). We evaluated the chemical composition, texture, water holding capacity, colour, fatty acids (FAs) profile, volatile compounds, and sensorial appraisal of the meat from 30 male suckling kids (cold carcass weight 4.74 kg, 4.82 kg, and 4.65 kg for DOP-0, DOP-40, and DOP-80, respectively) of the Payoya breed (n = 10 for each diet). Meat from kids in the DOP-40 and DOP-80 groups exhibited characteristics favourable for human health, including the meat&rsquo, s thrombogenicity index, PUFA/SFA ratio (0.60 index), and n-6 PUFA/n-3 PUFA ratio (approximately 7.50). The meat also exhibited reduced MUFA content (around 460 mg /100g fresh meat). An increase in ethyl furan, dimethyl disulphide and heptane was observed in grilled meat from goats that were fed using DOP. The inclusion of DOP in goat feed improved consumers&rsquo, sensory appreciation of the kid&rsquo, s meat.

[EN] The aim of this study was to establish the relationships between faecal fat concentration and gaseous emissions from pig slurry. Five diets were designed to meet essential nutrient requirements: a control and four experimental feeds including two levels (35 or 70g/kg) of calcium soap fatty acids distillate (CSP) and 0 or 200g/kg of orange pulp (OP) combined in a 2×2 factorial structure. Thirty growing pigs (six per treatment) were used to measure dry matter (DM) and N balance, coefficients of total tract apparent digestibility (CTTAD) of nutrients, faecal and urine composition and potential emissions of ammonia (NH3) and methane (CH4). Increasing dietary CSP level decreased DM, ether extract (EE) and crude protein (CP) CTTAD (by 4.0, 11.1 and 3.5%, respectively, P, This project was funded by the Spanish Ministry of Science and Innovation (AGL2011-30023) and the Valencian Government (ACOMP/2013/118). We also thank CAPES Foundation, Ministry of Education of Brazil, Brasilia - DF 70040-020, Brazil for a research fellowship grant.

The differences between the physicochemical (water content, water activity, pH, NaCl, fat, color, and texture) and sensory (descriptive analysis and consumer test) characteristics of cheeses made from the milk of goats fed on a typical control diet and goats fed on a diet incorporating orange pulp were compared. The addition of orange pulp leads to obtain cheeses(i) with a lower pH and water activity, but with a higher fat content, (ii) that are lighter and with a more yellowish-green hue and (iii) higher in color purity, hardness and adhesiveness, although they are less elastic and cohesive. Thus, the incorporation of orange pulp into the goat's diet affected not only the presence of holes in the cheese, but also its hardness, goat taste and salty taste, which were associated with a higher score of consumer acceptance. (C) 2014 Elsevier B.V. All rights reserved., The authors thank the panel of assessors for making themselves available and the Universitat Politecnica de Valencia for the financial support given throughout the Project PAID-05-12, SP20120723.