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We investigated the post-weaning effect of providing additional objects (i.e. rope, ball, tyre) to commercial piglets given straw pre- and post-weaning on behaviour and average daily gain (ADG). Piglets from litters with objects (O,n = 20 in 5 litters) and controls (C,n = 20 in 5 litters) were observed from 24 h post-weaning and during six days. Piglets were weighed at birth, weaning and 11 days post-weaning. O-piglets performed less littermate manipulation (P < 0.05), exploring pen fixtures (P < 0.1) and lying (P < 0.1) but spent more time in the creep post-weaning than C-piglets (P < 0.1). Social or locomotor play was expressed similarly in both treatments. Day strongly influenced the behaviour of pigs post-weaning. O-piglets had significantly lower pre-weaning ADG than C-piglets (P < 0.05), but there was no significant difference in post-weaning ADG between the treatments. Providing objects to piglets around weaning may reduce some negative behaviours, but may affect weight gain negatively.

The study was conducted between March and September 2019 in six meat-type turkey flocks with similar management standard procedures using the transect walk method. The concept of the method is based on visual observation of the birds while slowly walking across the entire farm in predetermined transects. Each flock was evaluated at three different times during the fattening cycle: at 3 to 4, 12 to 13, and 19 to 20 weeks of age, and total number of males and females that were immobile or lame, had visible head, vent, or back wounds, were small, featherless, dirty, or sick, had pendulous crop, or showed aggression toward birds or humans were recorded. At each visit, NH3 and CO2 were measured within the facilities. In the first assessment, the most frequently observed welfare indicators were small size (0.87%) and immobility (0.08%). Males showed a significantly higher prevalence of small size (p &lt, 0.01), sickness (p &lt, 0.05), and dirtiness (p &lt, 0.1) compared to females. In the second assessment, the most common findings in both sexes were dirtiness (1.65%) and poor feather condition (1.06%), followed by immobility (0.28%). Males were significantly dirtier (p &lt, 0.001), had more immobile birds (p &lt, 0.01) and birds with vent wounds (p &lt, 0.1), but had fewer sick birds (p &lt, 0.05). In the last assessment, an increase in immobile, lame, sick, and dead birds was recorded, indicating an increase in health problems. Higher CO2 (3000 and 4433 ppm) and NH3 (40 and 27.6 ppm) values were noted only at the first assessment in two facilities. Further analyses showed that slightly elevated NH3 and CO2 levels did not influence the occurrence of welfare indicators. This study is the first description of the welfare of commercial turkey flocks in Slovenia.

A dog&rsquo, s aggressive behavior is influenced by external and internal factors, including its psychological profile. In this study, dogs&rsquo, and owners&rsquo, personalities and the owners&rsquo, attachment style to their dogs were identified and associated with owner-reported dog aggression towards humans and animals. Forty Slovenian owners participated with their dogs, of different breeds and aggression history, sorted into three groups (non-aggressive dogs, dogs aggressive towards humans, and dogs aggressive towards animals). The owners filled out three separate questionnaires that assessed dog aggression history towards different targets, owner&rsquo, s personality and degree of insecure attachment styles to dogs, namely anxious and avoidant attachment. Dog personality was characterized using a standardized dog mentality assessment test, during which the dog was exposed to nine tasks, performed outside, and dogs were scored based on behaviors they exhibited. The results indicated that dogs which were aggressive towards humans were less sociable than non-aggressive dogs and this was associated with the higher neuroticism scores of their owners. We also found that dogs which were aggressive towards strangers had owners with lower scores for anxious attachment and that dogs which were aggressive towards owners had owners with higher scores for avoidant attachment. These results imply that the psychological profiles of both a dog and its owner influence dog aggression towards humans.

This study focused on stress physiology by characterizing cortisol responses to stressors in tail biters (n = 10), victims (n = 10), and control pigs (n = 10) of two different breeds (Landrace × Yorkshire, LY; Landrace/Yorkshire × Landrace/Duroc; LYLD) and sexes (females and castrated males). We exposed pigs to 10 min of isolation with a human at the farm and transported them to a controlled environment. There, the behaviour was registered for 10 min during sessions when subjected to a novel object test and to a novel arena test. Sampling times of salivary cortisol were in all the fear tests before testing and 60 min thereafter, while for transportation as well 120 min after the beginning of transportation. We additionally measured cortisol at 7:00 and 16:00 h during three days following transportation. The basal stress axis activity followed a distinct diurnal rhythm between sexes and breeds, with castrated males having higher cortisol level than females and LY pigs higher than LYLD pigs. Following isolation at the farm and transportation, the concentration of salivary cortisol was higher in LY than in LYLD pigs. Pigs considered the exposure to a novel arena, but not to a novel object, stressful by showing a cortisol level after testing higher than before testing. The results suggest a genotypic effect on sensitivity to stress in pigs that have performed tail biting, have been victimized, or have not been involved in tail biting.

We used eight horses 1 to 12 years old to investigate the influence of irregular feeding times on the behavior. The animals were housed in individual boxes, fed with hay ad libitum and barley-oat mixture at three set times; 05:00 (earlier feeding) on Thursdays, 07:00 (delayed feeding) on Saturdays and 06:00 (regular feeding) on other weekdays. Direct observations took place in 10 continuous weeks; they started 1 h prior to feeding and lasted for 2 h. Long-term behaviors (i.e. hay and concentrate consumption, resting) were recorded every 5 min, short-term behaviors (i.e. kicking, pawing the ground, comfort behavior, taking a look toward the door) were recorded continuously. Compared to the regular feeding time, horses spent less time consuming hay, more time resting and less often took a look toward the door during the earlier feeding, whereas during the delayed feeding horses more often performed pawing the ground, kicking, comfort behavior, and took a look toward the door (p < .05). Our results indicate that deviations from the regular feeding schedule affected the behavior of horses and compromised their temporal predictability.

In the present study, we investigated behavioural responses and determined parameters of heart rate variability (HRV) to elucidate a relative activation of autonomic nervous system (ANS) during baseline (10 min) and in response to potentially stressful situations (10 min) in two pig breeds and sexes. Gilts (n = 21) and barrows (n = 9) of the Landrace × Yorkshire (LY; n = 15) and Landrace/Yorkshire × Landrace/Duroc (LYLD; n = 15) breeds were subjected to a novel object test (NOT) and a novel arena test (NAT). Basal ANS state differed in pigs across breeds but not sexes. Landrace × Yorkshire pigs had a significantly lower basal heart rate (HR) and low-frequency band (LF) with a higher root mean square of successive interbeat intervals (RMSSD) and high-frequency band (HF) than LYLD pigs. In the NOT, despite having similar cardiac responses, gilts had a longer duration of contact with a novel object, higher lying and standing duration, and a lower duration of walking compared with barrows. In the NAT, we found similar behaviour across sexes but a different degree of ANS state, with barrows having a significantly higher increase in LF/HF (power of the low frequency component divided by the power of the high-frequency band) compared with gilts. Landrace/Yorkshire × Landrace/Duroc pigs showed longer duration of contact with a novel object in the NOT accompanied by less lying and standing than LY pigs in both tests. No difference in ANS activation between breeds was found in the NOT. In the NAT, HR increased more from baseline to testing in LY pigs than in LYLD pigs. There is a complex and often contradictory nature of relationships between behaviour and cardiac responses to novelty in pigs of different breeds and sexes.

Early handling of animals including tactile stimulation (TS) has been shown to have beneficial effects on the physical and psychological development of species where considerable maternal interaction, e.g. in the form of licking, already occurs. But little is known about the magnitude of these effects, if any, in species without this natural mechanism. Piglets from 13 litters ( N = 127) were subjected to four treatments: AH – all piglets in a litter received TS; NH – none of the piglets in a litter received TS; 50/50H – half of a litter received TS and 50/50NH – half of a litter did not receive TS. The TS was performed by a human stroking the back of the piglet for 2 min from 5 to 35 days of age. At 4 weeks of age the piglets were tested twice in an open-field/human-approach test, with either a familiar (F) or an unfamiliar person (U). Body weight was measured at birth, 5, 9 and 12 weeks of age. In the tests, AH and 50/50H piglets allowed more physical contact, regardless of the familiarity of the person (AH: 22.5 ± 2.3 F; 24.1 ± 2.3 U, 50/50H: 18.1 ± 2.2 F; 25.3 ± 2.2 U, P = 0.05). Additionally, AH piglets vocalized least and were least often in the perimeter zone (PZ) of the arena (AH grunts: 6.0 ± 0.8, P = 0.002; 12.8 ± 0.8, P = 0.0005; 18.1 ± 0.8, P = 0.08; AH PZ: 3.9 ± 0.8, P = 0.009; 9.1 ± 0.8, P = 0.003; 12.6 ± 0.7, P = 0.004 in isolation, stationary and moving person phase, respectively). In contrast, NH piglets vocalized most and were most often in the PZ. 50/50NH had higher body weights at 12 weeks than 50/50H, whereas AH and NH piglets were intermediate (AH: 36.9 ± 0.9, NH: 35.6 ± 1.0, 50/50H: 34.2 ± 1.3, 50/50NH: 37.8 ± 1.3, P = 0.03). This study suggests that early handling changed the way piglets reacted to challenging situations in that handled piglets showed behaviour suggesting they were less fearful in a novel environment and less fearful of being handled by people in general. Interestingly though, the early handling did not result in the same beneficial effects on the physical development of the piglets, as handled piglets were not heavier. Instead, it was the 50/50NH piglets that were the heaviest, implying that the daily presence of a human moving around in the pen to handle the 50/50H piglets seemed to stimulate weight gain.

The Slovenian karst region and similar types of land are difficult to cultivate and often exposed to the process of being abandoned and overgrown with shrubs, trees, and brushwood. Co-grazing in a mixed group may be a way to optimize the management of sheep and goat flocks in such areas. To obtain more knowledge of the natural behavior of small ruminants, the experiment was designed on pasture in the mountain karst region. The experimental area was divided in 6 paddocks, of which 3 paddocks were covered with grass, herbs, and legumes (i.e., grassy paddock [GP]). In the other 3 paddocks the area was additionally overgrown with hazel, beech trees, and bushes (i.e., woody paddock [WP]). In a mixed flock of 40 Slovenian local sheep breed (Istrian Pramenka) and 10 crossbreed goats (Saanen × Alpine goat), 10 animals per species were focally observed during daylight (0500-2100 h). The natural behavior was scored on 12 d, 2 consecutive days in each of the paddocks. Animals were rotated between 6 paddocks according to the balanced schedule. They stayed at each paddock for 5 or 6 d. Observations started on the third day after moving the animals into a specific paddock, following 2 d of adaptation. The results indicated that botanically diverse paddock together with climate conditions affected the behavior. Goats were grazing more (P < 0.001) whereas sheep less in the WP (P = 0.05). In sheep, drinking and salt consumption were higher (both P < 0.001) in the WP, suggesting that salt triggered additional water consumption. The WP enabled conditions where more comfort behavior, that is, autogrooming (both species P < 0.001) and object grooming (both species P < 0.001), was performed. On the second day of observation, animals grazed more compared to the first day (both species P < 0.001), most likely due to lower forage availability. Goats drank (P < 0.001) and consumed more salt on the second day (P < 0.001). With higher temperature sheep and goats grazed less (P < 0.05 and P < 0.001, respectively) and goats drank more (P < 0.05). Sheep showed more while goats less autogrooming when temperature was lower (both P < 0.001). More autogrooming was shown on the second day of observation but only in goats (P < 0.001). The performed behavior of co-grazing sheep and goats suggests different foraging strategies and adaptation between the species, which may be taken into account for management decisions under specific environmental conditions in the mountain karst region.

Made available in DSpace on 2018-12-11T16:40:23Z (GMT). No. of bitstreams: 0 Previous issue date: 2016-07-28 It is known that tactile stimulation (TS) during ontogeny modifies brain plasticity and enhances the motor and cognitive skills. Our hypothesis was that early handling including TS would increase play and exploratory behaviour in commercial pigs under standardized test conditions. Piglets from 13 litters were subjected to three handling treatments from 5 to 35 days of age: all the piglets were handled (H), none of the piglets were handled (NH) or half of the piglets in the litter were handled (50/50). At 42 days of age, the pigs' behaviour was observed in pairs in a novel pen with a 'toy' (tug rope). The main results were that more locomotor play was performed by pigs from litters where all or half of them had been handled, whereas social exploratory behaviour was more pronounced in pigs from litters where half of them had been handled. Although behaviour was affected by the interaction of treatment with sex or with weight category, we propose that the handling procedure does seem to have acted to increase locomotor skills and that handling half of the piglets in the litter may have triggered a series of socio-emotional interactions that were beneficial for the whole group. Department of Animal Environment and Health Swedish University of Agricultural Sciences, Box 7068 Animal Science Department São Paulo State University - Unesp, Via de Acesso Prof. Paulo Donato Castellane s/n Animal Science Department São Paulo State University - Unesp, Via de Acesso Prof. Paulo Donato Castellane s/n

The aim of the study was to investigate the feeding behaviour differences between Krskopolje pig (KP) and a modern hybrid (MH) pig. The behaviour was recorded during concentrate and hay consumption after the body weight of pigs was 100 kg. Animals (N=24) were observed in a straw-bedded system in eight pens of three pigs. During concentrate consumption, feeding, drinking and displacement at the trough (when a pig changed a feeding place) were directly recorded. During hay consumption, hay chewing, resting, exploring, playing and synchronisation while hay chewing were scored. MH ate faster and drank less than KP; however, they changed their feeding places more often and showed a higher probability to chew hay (overall P

In connection with the development of a test method for the attractiveness and appropriateness of nests for laying hens, we carried out an investigation by using a preference test and motivation measuring with the help of push doors. Hens were offered two different nest sites either consisting of a tray filled with wood shavings (litter tray) or a wooden nest box plus wood shavings (nest box). Hens were individually housed in pens (2.0 m × 2.0 m) and had free access to the nest sites until they laid their 15th egg. From that day the hens had to overcome a push door to reach the nest sites. Resistance for entrance was also increased stepwise at the door leading to the hens’ nest. The experiment ended when a hen stopped to lay in her usual nest site for four consecutive days (postexperimental period). The behaviour during the last hour before oviposition was video taped at a level of resistance of 3.5, 6.0, 7.5 and 10.0 N. The hens were categorized into nest and litter layers depending on nest choice. All but one hen pushed maximum resistances between 11.5 and 18.0 N with no differences between nest and litter layers. Behaviour did not significantly change with increasing levels of resistance, but there were significant differences between nest and litter layers. Nest layers spent more time nesting than litter layers while the latter showed a strong tendency to more exploring behaviour. For litter layers, more entries through the push door leading to their nest site and more unsuccessful pushes were detected than for nest layers. According to our results, two types of layers could be distinguished whereas the two were equally motivated to access their nest site.

When alternative husbandry systems to battery cages are used, a problem may arise whereby eggs are not laid in the nests provided. This leads not only to economic loss, but may also promote the outbreak of cannibalism. However, the choosing of a nest involves complex behavioural patterns and may be influenced, among other things, by exposure to a specific colour at an early age [Huber-Eicher, B., 2004. The effect of early colour preference and of a colour exposing procedure on the choice of nest colours in laying hens. Appl. Anim. Behav. Sci. 86, 63–76]. Chicks exposed to the colours blue, green and red, preferred yellow nests when in lay, whereas exposure to yellow resulted in an indifference towards the colour of nest. In the study reported here, we further investigated this effect of exposure to yellow. During the first 12 days of life, chicks were exposed to either red or yellow and to either a high or low light intensity (four treatment regimes in total). A hen's choice of nest colour was tested at the start of lay (weeks 20–23). We randomly selected 20 hens from each of the four treatment regimes. The 80 chosen hens were then tested in groups of four, each of the four hens coming from a different treatment regime. When given a choice of nests painted in the colours yellow, red, blue or green, hens exposed to red and to a high light intensity at an early age showed a significant preference for yellow nests. Hens exposed as chicks to red and a low light intensity, or to yellow and either high or low light intensity were indifferent towards nest colour. In conclusion, we demonstrate that the effect of early exposure to yellow on reducing the preference for yellow nests later on, is due to the colour yellow per se, and not the higher light intensity that goes with it. However, high light intensity in combination with red increases the preference for yellow nests.