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Effects of genetic merit on energy intake, milk yield, fat and protein percentages, BW, BW change, plasma concentration of bST, and plasma concentration of urea N were determined for 139 heifers. Heifers, daughters of bulls of high genetic merit (average +408 kg of PTA for milk) or of average genetic merit (average -153 kg of PTA for milk), were allotted to either a high or low energy diet. Heifers of high genetic merit yielded 8.1% more milk and had 7.7% higher bST concentration than did heifers of average genetic merit, which were 3% heavier than heifers of high genetic merit. There was no significant effect of genetic merit group on energy intake, plasma concentration of urea N, or percentages of fat and protein. Heifers fed the high energy diet consumed 35.1% more energy, yielded 11.8% more milk with a lower fat percentage, and weighed 3% more than did heifers fed the low energy diet. The high energy diet depressed bST concentration by 13.3% and plasma concentration of urea N by 14.2% compared with concentrations for heifers fed the low energy diet. Correlations among bST, BW, and energy intake were negative and significant. Correlations of bST concentration with milk yield, fat percentage, and protein percentage were not significant. Body weight, BW change, milk yield, and energy intake were negatively correlated with plasma concentration of urea N.

Practical relationships were determined between milk production, health, and reproduction with the amount and use of body fat in high producing lactating Holstein dairy cattle. Approximately 350 cows and heifers > 15 mo of age in a high producing herd were assigned body condition scores at monthly intervals for 24 mo. Production of 305-d FCM averaged 9541 kg (range 8826 to 10,818 kg). Body condition score at each of four calvings at 30, 60, 90, 120, and 305 DIM in each parity and loss in score in each lactation were summarized. Multiple regression related scores to milk and milk fat production, reproduction, and disease variables within and among lactations. No difference in score occurred at calving or at dry-off among parities. The range of body condition scores was less than is commonly reported; however, loss of condition increased with increasing parity from .3 in first lactation to .9 body condition score units in lactations > or = 4. The body condition score varied quadratically with DIM but, at a given DIM, was not related to the daily milk production on that DIM. Parity had a stronger relationship with milk and milk fat production than did body condition score. However, within lactation, body condition score at calving and the loss of score were related quadratically to milk production. No significant relationships of body condition score to the incidences of pyometra, metritis, retained placenta, cystic ovarian disease, AI per conception, days to first AI, or dystocia existed in this herd.

Diurnal changes in percents of inorganic phosphorus in plasma were measured in three Holstein cows fitted with indwelling jugular catheters. Blood was sampled 34 times over 48 h. Changes of inorganic phosphorus of blood plasma appeared to be related to patterns of feed consumption. In a second experiment, effects of diet, season of calving, stage of lactation, lactation number, and milk yield on inorganic phosphorus of plasma and milk were measured with 40 Holstein cows. Dietary treatments were 1.0% calcium, .31% phosphorus; 1.0% and .54%; 1.8% and .30%; 1.7% and .54%. Blood and milk samples were taken at wk 6 postpartum and every 5th wk thereafter. Inorganic phosphorus in plasma and milk was higher for cows in first lactation than multiparous cows. Cows which calved in November to December had the highest inorganic phosphorus in plasma but the lowest in milk. Month of year affected inorganic phosphorus in milk but not in plasma. Inorganic phosphorus in plasma increased as milk yields decreased and as inorganic phosphorus in milk decreased. Dietary phosphorus affected inorganic phosphorus in plasma but not in milk. The correlation between inorganic phosphorus in milk and plasma was -.15. These data indicate the limitations of using inorganic phosphorus in plasma or milk as sole or primary means of determining nutritional phosphorus status of lactating cows.

Systems analysis was used to study costs and returns of breeding dairy cows artificially or naturally. In the analysis were costs of semen, detection of estrus for artificial insemination, days open, and maintaining bulls. Also included were genetic abilities of sires, discount rates on expected returns, and probability of a female offspring reaching lactation. Transmission of genetic superiority of sires used artificially over sires used naturally to both daughter and grand-daughter was considered. Daughters of sires used artificially were superior to daughters of natural service sires by 227, 454, or 682kg (mature equivalent) milk. Breeding was started 60 days into lactation and continued for 15 cycles (21 days each) or until 90% of the cows would be expected to have conceived. Conception rate from artificial service was decreased by 5% of its rate for each subsequent breeding. For comparing artificial insemination and natural service, an initial conception rate for artificial insemination of 50%, cost of keeping a bull of $15 per cow per yr, semen cost of $8 per unit, costs for detection of estrus of $10 per cow per yr, discount rate of 10%, a 40% chance of obtaining a lactating heifer replacement per conception, a cost of an additional day open of $1.50, and income over feed costs of $.099 per kg were applied. Calving interval was 365 days for breeding by natural service. Each increase in genetic superiority of bulls used artificially over natural service bulls of 227 kg meant that the calving interval from artificial insemination could be 10 days longer with the same expected returns. Other factors in the analysis had less effect on the comparison of artificial insemination with natural service than the genetic abilities of sires. Investment in herd health (reproductive) programs to increase breeding efficiency appeared economically sound.

The objective was to determine the relationship between thawing time and post-thaw temperature when the final temperature of the semen was brought to 40 C. One ejaculate from each of 12 dairy bulls was packaged in .5-ml French straws. Semen in five straws from each ejaculate was thawed in a 35 c water bath for 12 s or 1 min. The straws were then immediately placed in water baths at 0, 10 or 20 C for 1 min and then transferred to a water bath at 40 C for 1 min. Control treatments were thawed at (1) 35 C for 1 min, plunged into a 35 C water bath for 1 min and transferred to a final water bath at 40 C for 1 min, and (2) thawed at 40 C for 1 min, plunged into 40 C water bath for 1 min and transferred to a final water bath at 40 C for 1 min. Semen was incubated at 40 C and evaluated at 0, 4 and 8 h of incubation for percentage of motile spermatozoa (%MOT) and percentage of spermatozoa with intact acrosomes (PIA). Semen thawed at 35 C for 12 s and held at 0 C before being warmed to 40 C sustained immediate acrosomal damage (P less than .05) due to a delay in complete thawing of the semen. Semen thawed at 35 C for 1 min, held at 0 C and then warmed to 40 C also sustained immediate acrosomal damage which was due to cold shock. Cold shock damage was greater than damage due to a delay in complete thawing, therefore, precautions should be taken to prevent post-thawing cold shock. The 10 C post-thaw treatment resulted in spermatozoal damage with both thawing times, however, the effects were latent since damage was not observed until the 4- and 8-h evaluations. The 20 C post-thaw treatment did not decrease (P greater than .05) % MOT or PIA with either thawing time.

Relationships between calf birth weight and the dam's subsequent 200- and 305-day yields of milk, fat, and total solids were investigated in a total of 1471 calvings from two experimental stations and 28 yr. Independent variables included in least-squares analyses were year of calving, sex of calf, and linear and quadratic effects of age of cow and calf birth weight. Sex of calf had no significant effect on any yield variable. Effects of age of cow and year of calving were significant and positive on all measures of yield. The relationship between calf birth weight and each yield measure was linear and positive for calf birth weights between 23 and 50 kg. Expected differences in 200-day yields of milk, fat, and solids were 15.2, 11.0, and 18.8% higher for 50 kg calf birth weight as compared to 30 kg calf birth weight. Corresponding differences in 305-day milk, fat, and solids were 9.4, 4.5, and 11.8%. Thus, yields of milk, fat, and solids in Holsteins increase with increasing calf birth weight.

Blood and milk were sampled and quarter California Mastitis Test scores were taken during four 2-wk periods from 45 lactating cows. Vitamin A and beta-carotene in plasma and milk were analyzed within 48 h after collection. Total vitamin A equivalent (2 X amount of beta-carotene plus amount of vitamin A) also was calculated. Additionally, the total amount of vitamin A and beta-carotene excreted in milk was calculated from concentrations of vitamin A and beta-carotene in milk and milk weights recorded on sampling day. Independent effects of lactation number, period of collection, days in lactation, and California Mastitis Test Scores (the highest test score of four quarters used in the analysis) were examined by least-squares procedures using each blood and milk measure as the dependent variable. Results showed a highly significant independent effect of California Mastitis Test Scores for concentrations of plasma vitamin A, beta-carotene, and total vitamin A equivalent. Cows with lower plasma vitamin A, beta-carotene, and total vitamin A equivalent had higher test scores than cows with higher vitamin A and beta-carotene. Similar comparisons for amount of milk vitamin A, beta-carotene, and total vitamin A equivalent and total amount of each component excreted in milk showed no significant independent effects attributed to California Mastitis Test. Therefore, low concentrations of vitamin A and beta-carotene in plasma were associated with severity of mastitis in cows.

Various regression equations for estimating solids-not-fat in herd milk samples were calculated from 12,826 samples from 300 herds in one data source and 356 samples from 29 herds in another. Accuracy of estimating solids-not-fat is about the same when any two of the three independent variables (number of beads down, milk fat per cent, and per cent protein) are part of the regression equation. Little increase in accuracy was obtained when quadratic independent variables were included in the equations. Equations involving beads and protein per cent were slightly more accurate than equations involving beads and milk fat per cent. Fitting separate equations to different milk fat percentages appreciably improved the accuracy of estimating solids-not-fat only when the equation did not include the milk fat per cent as an independent variable. These results indicate the choice of the equation among those used in this study to estimate solids-not-fat in milk could be made by weighing the operational costs of the various equations rather than the predictive accuracy of the equation.

An on-campus intern program is used as an aid in teaching dairy cattle management. The program involves 12 students who operate and manage a herd of 30 cows. The program offers participating students the opportunity to acquire experience and skill in dairy husbandry and herd management, to accept and discharge responsibility, and to develop leadership and interpersonal relationship skills. The program has met with high success and is supported widely by students and industry.

Holstein calves were assigned to treatments of 1) pelleted prestarter (22% protein and 12% fat) and starter containing 10% alfalfa; 2) prestarter and starter containing 20% alfalfa; 3) no prestarter and starter containing 10% alfalfa; and 4) no prestarter and starter containing 20% alfalfa. Calves assigned to treatments 1 and 2 were fed 3.64 kg milk/d for 2 wk and calves assigned to treatments 3 and 4 were fed 3.64 kg milk/d for 3 wk and 1.82 kg milk/d for wk 4. Calves on treatments 2 and 3 were heavier at 10 wk but body weights and heights were similar by 6 mo. Rumen fluid and plasma measures were similar among treatments. Bull calves were assigned to treatments 1 and 3 and necropsied at 3 or 6 wk. Dry feed intakes to 3 wk and volatile fatty acid concentrations were greater for calves on treatment 1 than those on treatment 3. Wet weights of the empty reticulorumens were greater for calves on treatment 1 than those on treatment 3. Papillary development was not affected by weaning system. Calves weaned at 17 d and fed a prestarter have earlier rumen development than calves fed no prestarter and weaned later.

Effects of prepartum dietary crude protein on growth hormone, insulin in blood plasma, and subsequent reproduction and lactation were investigated in 30 Holsteins. Cows were fed either 80 or 100% of the crude protein recommended by National Research Council starting about 60 days precalving. Postcalving all animals were fed according to recommendations. Blood was sampled on days 220 (start of experiment), 250, 270, and 280 of gestation and on days 10 and 30 postpartum. Cows fed 80% crude protein prepartum showed decreased intake of dry matter and urea nitrogen in blood plasma prepartum, and yields of milk and total solids decreased by about 15%. Body weight changes through 30 days postpartum were not significantly different between treatments. There were no significant differences for growth hormone and insulin except growth hormone tended to be higher on day 270 of gestation for cows fed 80% crude protein. Insulin was negatively correlated with lactation number −.51 to −.56. Reproductive efficiency was similar between treatment groups. Lower crude protein intake during the dry period decreased dry matter intake and yields of milk and total solids.

The effect of four levels of progesterone on the occurrence and intensity of estrous behavior was determined in ovariectomized cows. Twelve Holsteins were bilaterally ovariectomized at least 2 mo before treatment. Progesterone (P4) was administered im two times/day for 5 d followed 72 h later with 2 mg estradiol (E2) im to induce estrous behavior. Treatments were control = oil, 100 mg P4/d, 300 mg P4/d and 500 mg P4/d. Each animal received all P4 treatments in a 4 X 4 Latin-square design. From d 2 through 6, with d 1 being the first day of P4 treatment, serum P4 concentrations averaged .3, 2.5, 6.1 and 12.3 ng/ml in treatments I to IV, respectively. Progesterone levels at the time of peak E2 (d9) were less than 1 ng/ml for treatments I, II and III and 1.8 ng/ml for treatment IV. Peak E2 levels for all groups averaged 23.9 pg/ml (12 h after E2 injection). Cows were observed continuously for 48 h after E2 injection to quantitate estrous behavior. Behavioral traits measured were: mounts initiated, mounts received, standing when mounted, chin rests initiated and chin rests received. Progesterone treatment caused a decrease in frequency of all five traits measured. As P4 levels increased, there was a linear (P less than .01) decrease in frequency of a behavioral trait. The percentage of the cows that showed each behavioral trait and mean time to first occurrence of each trait were not different after progesterone treatment, with the exception of standing behavior.(ABSTRACT TRUNCATED AT 250 WORDS)

Semen from each of 10 Holstein bulls was extended to 15 X 10(6) total spermatozoa per dose in egg yolk-Tris and in egg yolk-Tris containing 20% (vol/vol) heat-treated heifer serum. Semen was used during 12 mo by 11 herdsmen-inseminators in four commercial dairy herds to inseminate 2820 Holstein cows on the first postpartum service. Percentage conception of cows free of clinical disorders at breeding was determined by rectal palpation at 30 to 60 days after insemination. Addition of serum to the extender did not influence percentage conception. A significant interaction of bull x seminal treatment was found. Least squares means of percentage conception to first service ranged from 45.3 to 59.9% among the 10 bulls. When bulls were classified as either high (greater than 55%), average (50 to 55%), or low conception (less than 50%), approximately 200 first services were required to classify correctly 80 to 90% of the bulls. Least squares means of conception rates ranged from 40.1 to 62.7% among the 11 herdsmen-inseminators. 1) Addition of heat-treated homologous heifer serum to the extender does not influence conception in a predictable fashion; 2) fertility inherent to the bull significantly influences conception; and 3) inseminator skill affects conception greatly.

Effects of management and environmental factors on reproductive performance were evaluated as part of a fertility experiment in four large commercial dairy herds. The herds were selected because they utilized routine herd health programs, kept complete reproduction records on all cows, were enrolled in Dairy Herd Improvement, and would follow the protocol of the experiment. Only data from first services following parturition were utilized, and conception was determined by rectal palpation. Information on over 2800 experimental breedings was collected during 13 mo. Percentage conception to first service was not affected by milk production during current or previous lactations. However, cows with higher production during current or previous lactations. However, cows with higher production during current or previous lactation had longer intervals to first service. Previous days dry did not affect conception or interval to first service significantly. Conception was less for cows with less than 50 days to first service (32%) than for cows with over 50 days to first service (49 to 57%). No differences were found among 10-day intervals to first service of over 50 days. Cows in third and later lactation had lower reproductive performance than cows in first or second lactation. Month of breeding did not affect conception significantly but did affect interval to first service. However, no seasonal trends were apparent. Differences among herds were significant for both conception and interval to first service.

We assessed the damage to frozen semen packaged in French straws stored for 2 yr in 60 on-the-farm semen tanks on 56 Washington dairy farms. Percentage of motilic spematozoa and percentage of spermatoza with intact acrosomes were not affected significantly by position of the semen on the rack (top or bottom goblet). The semen in 3 of the 60 tanks was lost to tank failure.

Immunoglobulin G1 concentration (IgG1) was measured in presuckle colostrum and calf serum obtained at 36 h and at weaning from inbred and straightbred Angus, Brangus, Hereford, Red Angus and Simmental cattle. Sources of variation considered as dam traits examined for IgG1 in colostrum and 36-h calf serum included line of sire, sire within line, age, and linear regression of IgG1 on inbreeding of dam. Only line of sire and inbreeding of dam were significant in the analysis of 36-h calf serum. Sources of variation considered as calf traits examined for IgG1 in calf serum at 36 h and at weaning included line of sire, sire within line, sex of calf, age of dam, and regressions of calf serum IgG1 on inbreeding of the calf and on dam's colostral IgG1. Only sire within line and the regression on dam's colostral IgG1 were significant for calf serum IgG1 at 36 h. Large differences existed in 36-h calf serum IgG1 between sires both within lines and when lines were ignored. Calves with 36-h serum IgG1 of less than 10 mg/ml were two to four times more likely to die before weaning than calves with higher IgG1 levels. The heritability estimates of IgG1 by paternal half-sib analysis were .41 +/- .30 for colostrum measured as a trait of the dam and .56 +/- .25 for 36-h. calf serum and .05 +/- .17 for calf serum at weaning considered as a trait of the calf. These estimates indicate that IgG1 in colostrum and 36-h calf serum could be increased by selection.(ABSTRACT TRUNCATED AT 250 WORDS)

Presuckle colostral samples and lamb serum samples taken 36 h postpartum were assayed for immunoglobulin G1 (IgG1) concentration (mg/ml) using single radial immunodiffusion. Breeds sampled included Polypay (P), Rambouillet (R), Targhee (T), Columbia (C), Finnish Landrace (F) and Finn crosses (Fx). Sources of variation examined in IgG1 concentration in colostrum (dam trait) included dam's sire breed, dam's sire, age of ewe and number of lambs born. All sources of variation were statistically significant. Least-squares means of IgG1 levels for sire breed were 80, 64, 67, 64, 72 and 69 mg/ml for P, R, T, C, F and Fx breed groups, respectively. A fetal stimulus may exist to increase the mass of IgG1 in colostrum available for multiple births (61, 69 and 77 mg/ml for single, twin and triplet, respectively). Ewe age was a significant source of variation because of a high mean concentration of IgG1 in the yearling's colostrum (100 mg/ml), whereas only slight differences occurred between the other age groups (65 to 67 mg/ml), except for the 7-yr older group (53 mg/ml). Sources of variation examined in IgG1 concentration of lamb serum at 36 h postpartum (lamb trait) included lamb's sire breed, lamb's sire, age of dam, birth type and sex, with dam's colostral IgG1 concentration and day born as covariates. Sire within breed, birth type and the two covariates were significant. Least squares means for sire breed were 36, 32, 33, 32, 31 and 32 mg/ml of serum for P, R, T, C, F and Fx groups, respectively. Lamb serum IgG1 decreased as birth type increased. The heritability of IgG1, estimated by paternal half-sib analyses, was .19 +/- .12 for colostrum and .18 +/- .06 for lamb serum.

The objective of this study was to compare conception to artificial insemination (AI) services in dairy cattle when semen was deposited into the uterine body or into both uterine horns (cornual insemination). Nine herdsman inseminators (HI) in four commercial dairy herds in Washington constituted the experimental units. Herds ranged in size from 393 cows to 964 cows. The duration of the experiment was 12 mo in three herds and 18 mo in the fourth herd. At the beginning of the experiment all inseminators were trained to deposit semen in the body of the uterus. Inseminators were instructed to use this method for 6 mo. Following employment of body deposition, the same inseminators were retrained to deposit one-half of the semen into the right uterine horn and one-half into the left uterine horn. Cornual inseminations were performed for 6 mo. A total of 4,178 services constituted the data set. Milk samples were collected from cows on the day of insemination and later were assayed for progesterone (P4). There was variation (P less than .01) in conception associated with month of insemination and insemination method (P less than .001). The monthly variation was not associated with season of the year. Least squares means for conception when semen was deposited in the uterine body was 44.7%, compared with 64.6% when cornual insemination was employed. The insemination treatment X inseminator interaction was not significant. Results suggest that cornual insemination provides an alternative to deposition of semen in the uterine body.