1. Culture of glochidia of the freshwater pearl mussel Hyriopsis myersiana (Lea, 1856) in artificial media
- Author
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Kovitvadhi Uthaiwan, Jorge Machado, and Napavarn Noparatnaraporn
- Subjects
biology ,Ecology ,media_common.quotation_subject ,Aquatic Science ,Hyriopsis myersiana ,biology.organism_classification ,Bivalvia ,Oreochromis ,Animal science ,Freshwater pearl mussel ,Artificial culture ,Juvenile ,Metamorphosis ,Mollusca ,media_common - Abstract
The freshwater pearl mussel, Hyriopsis myersiana (Limnoscapha) (Lea, 1856) was cultured in two artificial media at 23±2°C. Each artificial medium contained a mixture of M199, (Life Technologies, No. 71N0262) horse serum or fish ( Oreochromis niloticus ) artificial medium plasma as a protein source, and antibiotics/antimycotics at a ratio of 2:1:0.5. Glochidia were reared until they became juveniles, i.e. until the mantle and foot could be observed under a light microscope. The duration of glochidia development until the juvenile stage was 9–10 days in both media. After 1 month of controlled feeding with phytoplankton, the juveniles showed an elongate of shell with several growth lines. The more suitable artificial culture formula for the transformation from glochidia to juvenile stage was the medium containing protein from fish plasma. Survival from glochidia to juvenile stage was up to 85.3±3.9% in fish plasma, while it was equal to 46.2±12.7% in horse serum. The transformation from glochidia to juvenile stage was up to 84.3±2.3% in fish plasma, while it was equal to 44.3±8.9% in horse serum. Percentage survival and transformation from glochidia to juvenile stage were significantly higher in fish plasma than in horse serum ( P
- Published
- 2001